Issue 10, 2014
From the journal:

Molecular BioSystems

A metabonomic study of adjuvant-induced arthritis in rats using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

Yao Qi,ab   Zifeng Pi,*a   Shu Liu,a   Fengrui Song,a   Na Linc  and  Zhiqiang Liu*a  

* Corresponding authors

a Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
E-mail: mslab21@ciac.ac.cn, liuzq@ciac.ac.cn
Fax: +86-431-85262236
Tel: +86-431-85262236

b University of the Chinese Academy of Sciences, Beijing 100039, China

c Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China

Abstract

Rheumatoid arthritis (RA) is a chronic systemic inflammatory and autoimmune disease accompanied by the destruction and deformities of joints. Adjuvant-induced arthritis (AIA) is one of the excellent animal models of RA used to understand disease pathogenesis and screen potential drugs. In this paper, a urinary metabonomics method based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) has been established to investigate the disease progression of AIA and find potential biomarkers of secondary inflammation in AIA rats. 24 potential biomarkers were identified, including xanthurenic acid, kynurenic acid, 4-pyridoxic acid, and phenylalanine, which revealed that tryptophan metabolism, phenylalanine metabolism, gut microbiota metabolism and energy metabolism were disturbed in AIA rats. These potential biomarkers and their corresponding pathways are helpful to further understand the mechanisms of AIA and pathogenesis of RA. This study demonstrates that metabonomics based on UPLC-Q-TOF-MS is a powerful methodology to analyze the underlying disease pathogenesis.

Graphical abstract: A metabonomic study of adjuvant-induced arthritis in rats using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

About
Cited by
Related
Download options Please wait...

Article information

DOI
https://doi.org/10.1039/C4MB00131A
Article type
Paper
Submitted
06 Mar 2014
Accepted
08 Jul 2014
First published
11 Jul 2014

Mol. BioSyst., 2014,10, 2617-2625

Permissions

Request permissions

A metabonomic study of adjuvant-induced arthritis in rats using ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

Y. Qi, Z. Pi, S. Liu, F. Song, N. Lin and Z. Liu, Mol. BioSyst., 2014, 10, 2617 DOI: 10.1039/C4MB00131A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements