Issue 47, 2013
From the journal:

Journal of Materials Chemistry B

Nanoporous membrane based impedance sensors to detect the enzymatic activity of botulinum neurotoxin A†

Weiwei Ye,a   Jiubiao Guo,b   Sheng Chen*b  and  Mo Yang*a  

* Corresponding authors

a Interdisciplinary Division of Biomedical Engineering, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, P. R. China
E-mail: Mo.Yang@polyu.edu.hk
Fax: +852 2334 2429
Tel: +852 2766 4946

b Department of Applied Biology and Chemistry, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, P. R. China
E-mail: sheng.chen@polyu.edu.hk
Fax: +852 2364 9932
Tel: +852 34008795

Abstract

Botulinum neurotoxins are among the most potent toxic bacterial proteins for humans and there is a great need to develop simple, rapid and sensitive methods for toxin detection and protease activity quantification in field deployment. In this paper, a nanoporous membrane based impedance sensor was developed to monitor the activity of the BoNT serotype A light chain protease (LcA). Synaptosomal-associated protein 25 (SNAP-25) was first immobilized inside nanopore walls via silane linkers. BoNT LcA was then injected over the nanoporous membrane substrate sensor and specifically cleaved SNAP-25. The cleavage activity could be monitored by measuring impedance signals across nanoporous membranes which represented the nanopore blockage degree. This initial device could achieve a 500 pM LcA detection limit within 25 minutes.

Graphical abstract: Nanoporous membrane based impedance sensors to detect the enzymatic activity of botulinum neurotoxin A

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Article information

DOI
https://doi.org/10.1039/C3TB21152E
Article type
Paper
Submitted
15 Aug 2013
Accepted
15 Oct 2013
First published
17 Oct 2013

J. Mater. Chem. B, 2013,1, 6544-6550

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Nanoporous membrane based impedance sensors to detect the enzymatic activity of botulinum neurotoxin A

W. Ye, J. Guo, S. Chen and M. Yang, J. Mater. Chem. B, 2013, 1, 6544 DOI: 10.1039/C3TB21152E

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