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Alternative splice variant of actinin-4 in small cell lung cancer

Abstract

Tumor-associated alternative RNA splicing has gained considerable attention. We identified a novel alternative splice variant RNA of actinin-4 in human small cell lung caner (SCLC). Expression of the splice variant was highly specific to SCLC cell lines (10/10), biopsies (3/3), and testis. The variant encoded a peptide with a three amino-acid change in exon 8, where the germline missense mutation takes place in familial focal segmental glomerulosclerosis (FSGS). The variant protein showed high affinity to filamentous actin polymers and was not localized with cortical actin. Alternatively spliced actinin-4 may be a new diagnostic marker of SCLC and a candidate target for selective therapy.

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References

  • Araki N, Hatae T, Yamada T and Hirohashi S . (2000). J. Cell Sci., 113, 3329–3340.

  • Arora VK, Singh N, Chaturvedi S and Bhatia A . (2003). Acta Cytol., 47, 216–220.

  • Behrens J, Frixen U, Schipper J, Weidner M and Birchmeier W . (1992). Semin. Cell Biol., 3, 169–178.

  • Carney DN, Gazdar AF, Nau M and Minna JD . (1985). Semin. Oncol., 3, 289–303.

  • Djinovic-Carugo K, Gautel M, Ylanne J and Young P . (2002). FEBS Lett., 513, 119–123.

  • dos Santos NR, Torensma R, de Vries TJ, Schreurs MW, de Bruijn DR, Kater-Baats E, Ruiter DJ, Adema GJ, van Muijen GN and van Kessel AG . (2000). Cancer Res., 60, 1654–1662.

  • Echchakir H, Mami-Chouaib F, Vergnon I, Baurain J, Karanikas V, Chouaib S and Coulie PG . (2001). Cancer Res., 61, 4078–4083.

  • Hirohashi S and Kanai Y . (2003). Cancer Sci., 94, 575–581.

  • Honda K, Yamada T, Endo R, Ino Y, Gotoh M, Tsuda H, Yamada Y, Chiba H and Hirohashi S . (1998). J. Cell Biol., 140, 1383–1393.

  • Jager E, Jager D and Knuth A . (1999). Cancer Metastasis Rev., 18, 143–150.

  • Kaplan JM, Kim SH, North KN, Rennke H, Correia LA, Tong HQ, Mathis BJ, Rorriguez-Perez JC, Allen PG, Beggs AH and Pollak MR . (2000). Nat. Genet., 24, 251–256.

  • Levi F, La Vecchia C, Lucchini F and Negri E . (1996). Eur. J. Cancer, 32A, 652–672.

  • Michaud JL, Lemieux LI, Dube M, Vanderhyden BC, Robertson SJ and Kennedy CR . (2003). J. Am. Soc. Nephrol., 5, 1200–1211.

  • Nikolopoulos SN, Spengler BA, Kisselbach K, Evans AE, Biedler JL and Ross RA . (2000). Oncogene, 19, 380–386.

  • Shimosato Y, Nakajima T, Hirohashi S, Morinaga S, Terasaki T, Yamaguchi K, Saijo N and Suemasu K . (1986). Cancer Lett., 33, 241–258.

  • Small JV, Rottner K and Kaverina I . (1999). Curr. Opin. Cell Biol., 11, 54–60.

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Acknowledgements

This study was supported by grants from the Ministry of Health, Labor and Welfare and by the Program for Promotion of Fundamental Studies in Health Sciences of the Organization for Pharmaceutical Safety and Research of Japan.

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Correspondence to Tesshi Yamada.

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Honda, K., Yamada, T., Seike, M. et al. Alternative splice variant of actinin-4 in small cell lung cancer. Oncogene 23, 5257–5262 (2004). https://doi.org/10.1038/sj.onc.1207652

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