Abstract
Tumor-associated alternative RNA splicing has gained considerable attention. We identified a novel alternative splice variant RNA of actinin-4 in human small cell lung caner (SCLC). Expression of the splice variant was highly specific to SCLC cell lines (10/10), biopsies (3/3), and testis. The variant encoded a peptide with a three amino-acid change in exon 8, where the germline missense mutation takes place in familial focal segmental glomerulosclerosis (FSGS). The variant protein showed high affinity to filamentous actin polymers and was not localized with cortical actin. Alternatively spliced actinin-4 may be a new diagnostic marker of SCLC and a candidate target for selective therapy.
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Acknowledgements
This study was supported by grants from the Ministry of Health, Labor and Welfare and by the Program for Promotion of Fundamental Studies in Health Sciences of the Organization for Pharmaceutical Safety and Research of Japan.
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Honda, K., Yamada, T., Seike, M. et al. Alternative splice variant of actinin-4 in small cell lung cancer. Oncogene 23, 5257–5262 (2004). https://doi.org/10.1038/sj.onc.1207652
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DOI: https://doi.org/10.1038/sj.onc.1207652
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