Abstract
Objective: To explore the feasibility of low-fat spreads as vehicles for folic acid (FA) fortification by determining the acute absorption of FA from a fortified spread.
Design: Double blind, crossover study to test each of the following treatments administered at 1-weekly intervals: (A) 20 g low-fat (40%) spread fortified with 200 μg FA and a placebo tablet; (B) 20 g low-fat placebo spread and a 200 μg FA tablet; (C) 20 g low-fat placebo spread and a placebo tablet.
Subjects: A total of 13 male volunteers, aged 31.8±13.2 y.
Main outcome measures: Plasma total folate concentrations, measured before and up to 10 h after each treatment (n=10 samples per treatment).
Results: Plasma folate concentrations were significantly increased compared with baseline values 1 h after administration of the FA tablet, and 1.5 h after the FA spread, and remained significantly higher than the baseline values for up to 7 h after both treatments. The maximum plasma folate response (Rmax), corrected for baseline values and ‘placebo response’, was established between 1 and 3 h postprandially in response to both FA spread and FA tablet, and no significant difference in Rmax was found between the two treatments (13.4 vs 14.4 nmol/l, P=0.9). The acute absorption of FA from fortified spread relative to that from the tablet, calculated on the basis of area under the plasma folate response curve, was 67% (P=0.04).
Conclusion: The absorption of FA from fortified low-fat spread, although lower than from a tablet, is effective. These results suggest that low-fat spreads, typically associated with fat-soluble vitamin fortification, may also be considered feasible as vehicles for FA fortification.
Sponsorship: Unilever, The Netherlands.
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References
Altman DJ (1991): Serial measurements. In Practical Statistics for Medical Research, ed. DJ Altman, pp 426–433. London: Chapman & Hall.
Bates CJ, Pentieva KD, Matthews N & Macdonald A (1999): A simple, sensitive and reproducible assay for pyridoxal 5-phosphate and 4-pyridoxic acid in human plasma. Clin. Chim. Acta 280, 101–111.
Chanarin I (1979): Transport of folate. In The Megaloblastic Anaemias, 2nd Edition, ed. I Chanarin, pp 178–471. Oxford : Blackwell Scientific.
Choi SW & Mason JB (2000): Folate and carcinogenesis: an integrated scheme. J. Nutr. 130, 129–132.
Colman N, Larsen JV, Barker M, Barker AE, Green R & Metz J (1975): Prevention of folate deficiency by food fortification. III. Effect in pregnant subjects of varying amounts of added folic acid. Am. J. Clin. Nutr. 28, 465–470.
Cuskelly GJ, McNulty H & Scott JM (1996): Effect of increasing dietary folate on red-cell folate: implications for preventions of neural tube defects. Lancet 347, 657–659.
Czeizel AE & Dudas I (1992): Prevention of the first occurrence of neural-tube defect by periconceptional vitamin supplementation. N. Engl. J. Med. 327, 1832–1835.
De Bree A, van Dusseldorp M, Brouwer IA, van het Hof KH & Steegers-Theunissen RPM (1997): Folate intake in Europe: recommended, actual and desired intake. Eur. J. Clin. Nutr. 51, 643–660.
Department of Health (1991): Report on Health and Social Subjects 41. Dietary Reference Values for Food Energy and Nutrients for the United Kingdom. London: The Stationary Office.
Eikelboom JW, Lonn E, Genest Jr J, Hankey G & Yusuf S (1999): Homocyst(e)ine and cardiovascular disease: a critical review of the epidemiologic evidence. Ann. Intern. Med. 131, 363–375.
Eitenmiller RR & Landen Jr WO (1999): Folate. In Vitamin Analysis for the Health and Food Sciences, eds RR Eitenmiller & WO Landen Jr, pp 411–465. Boca Raton, FL: CRC Press.
Frosst P, Blom HJ, Milos R, Goyette P, Sheppard CA, Matthews RG, Boers GHJ, den Heijer M, Kluijtmans LAJ, van den Heuvel P & Rozen R (1995): A candidate genetic risk factor for vascular disease: a common mutation in methylenetetrahydrofolate reductase. Nat. Genet. 56, 111–113.
Gregory JF III (1997): Bioavailability of folate. Eur. J. Clin. Nutr. 51(Suppl 1), S54–S59.
Holland B, Welch AA, Unwin ID, Buss DH, Paul AA & Southgate DAT (1991): McCance and Widdowson's The Composition of Foods, 5th Edition. Cambridge: Royal Society of Chemistry & MAFF.
Honein MA, Paulozzi LJ, Mathews TJ, Erickson JD & Wong LYC (2001): Impact of folic acid fortification of the US food supply on the occurrence of neural tube defects. JAMA 285, 2981–2986.
Jacques PF, Selhub J, Bostom AG, Wilson PWF & Rosenberg IH (1999): The effect of folic acid fortification on plasma folate and total homocysteine concentrations. N. Engl. J. Med. 340, 1449–1454.
Kang SS, Wong PWK & Malinow MR (1992): Hyperhomocyst(e)inemia as a risk factor for occlusive vascular disease. Annu. Rev. Nutr. 12, 279–298.
Kelleher B & O'Broin S (1991): Microbiological assay for vitamin B12 performed in 96-well microtitre plates. J. Clin. Pathol. 44, 592–595.
Leino A (1999): Fully automated measurement of total homocysteine in plasma and serum on the Abott Imx analyser. Clin. Chem. 45, 569–571.
McNulty H (2001): Increasing evidence in favour of mandatory fortification with folic acid. Br. J. Nutr. 86, 425–426.
McNulty H, Cuskelly GJ & Ward M (2000): Response of red blood cell folate to intervention: implications for folate recommendations for the prevention of neural tube defects. Am J. Clin. Nutr. 71(Suppl. 5), 1308S–1311S.
Molloy AM & Scott JM (1997): Microbiological assay for serum, plasma, and red cell folate using cryopreserved, microtiter plate method. Methods Enzymol. 281, 43–53.
Mount JN, Heduan E, Herd C, Jupp R, Kearney E & Marsh A (1987): Adaptation of coenzyme stimulation assays for the nutritional assessment of vitamins B1, B2 and B6 using the Cobas Bio centrifugal analyser. Ann. Clin. Biochem. 24, 41–46.
MRC Vitamin Study Research Group (1991): Prevention of neural tube defects: results of MRC Vitamin Study. Lancet 338, 132–137.
Pfeiffer CM, Rogers LM, Bailey LB & Gregory III JF (1997): Absorption of folate from fortified cereal-grain products and of supplemental folate consumed with or without food determined by using a dual-label stable-isotope protocol. Am. J. Clin. Nutr. 66, 1388–1397.
Pietrzik K, Hages M & Remer T (1990): Methodological aspects in vitamin bioavailability testing. J. Micronutr. Anal. 7, 207–222.
Prinz-Langenohl R, Bronstrup A, Thorand B, Hages M & Pietrzik K (1999): Availability of food folate in humans. J. Nutr. 129, 913–916.
Tamura T, Mizuno Y, Johnston KE & Jacob RA (1997): Food folate assay with protease, α-amylase, and folate conjugase treatments. J. Agric. Food Chem. 45, 135–139.
Tucker KL, Selhub J, Wilson PWF & Rosenberg IH (1996): Dietary intake pattern relates to plasma folate and homocysteine concentrations in the Framingham heart study. J. Nutr. 126, 3025–3031.
Ward M, McNulty H, McPartlin J, Strain JJ, Weir D & Scott JM (1997): Plasma homocysteine, a risk factor for cardiovascular disease, can be effectively reduced by physiological amounts of folic acid. Q. J. Med. 90, 519–524.
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We wish to acknowledge the volunteers who kindly participated in the study.
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Guarantor: Professor H McNulty.
Contributors: KP participated in the study design and was responsible for statistical analysis, interpretation of the data and writing the paper. DMcK was responsible for study execution and laboratory analysis. ND participated in recruitment of the subjects, study execution, sample collection and laboratory analysis. EAMD was involved in hypothesis development, study design and provision of the product. RGJMJ was responsible for hypothesis development, study design and provision of the product. NMJP participated in hypothesis development, study design and provision of the product. HMcN was responsible for planning the study and study design and participated in the statistical analysis, interpretation of the data and writing the paper.
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Pentieva, K., McKillop, D., Duffy, N. et al. Acute absorption of folic acid from a fortified low-fat spread. Eur J Clin Nutr 57, 1235–1241 (2003). https://doi.org/10.1038/sj.ejcn.1601674
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DOI: https://doi.org/10.1038/sj.ejcn.1601674
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