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Molecular characterization of an adenoviral vector resulting from both homologous and nonhomologous recombination

Cancer Gene Therapy volume 6, pages 475–481 (1999)Cite this article

Abstract

Recombinant adenoviral vectors are being used extensively for gene transfer. During the construction of an E1-deleted virus expressing the human B7-1 gene, an aberrant recombinant (Ad.ihB7-1) arose with an unusual 5′ sequence. Characterization and sequencing of Ad.ihB7-1 showed that its structure was the result of both homologous and nonhomologous events. The most striking features of the construct were the incorporation of bacterial genomic DNA, an additional inverted terminal repeat, and portions of E1a. The appearance of this construct has implications for vector design and indicates the need for careful analysis and characterization of recombinant adenoviral vectors for clinical use.

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  1. Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Medical Center, Philadelphia, 19104, Pennsylvania

    Jason G Smith & Stephen L Eck

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  1. Jason G Smith
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  2. Stephen L Eck
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Correspondence to Stephen L Eck.

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Smith, J., Eck, S. Molecular characterization of an adenoviral vector resulting from both homologous and nonhomologous recombination. Cancer Gene Ther 6, 475–481 (1999). https://doi.org/10.1038/sj.cgt.7700062

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  • DOI: https://doi.org/10.1038/sj.cgt.7700062

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