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British Journal of Cancer (2006) 94, 1007-1010.
doi:10.1038/sj.bjc.6603050 Published online 28 March 2006
Lack of evidence of disease contamination in ovarian tissue harvested for cryopreservation from patients with Hodgkin lymphoma and analysis of factors predictive of oocyte yield
T Seshadri1, D Gook2, S Lade3, A Spencer4, A Grigg5, K Tiedemann6, J McKendrick7, P Mitchell8, C Stern2 and J F Seymour3
1Monash Medical Centre, Clayton, Victoria 3168, Australia
2Royal Women's Hospital, and Melbourne IVF, East Melbourne, Victoria 3002, Australia
3The Peter MacCallum Cancer Centre, Department of Haematology and Medical Oncology Level 5, Locked Bag 1, A'Beckett st, East Melbourne, Victoria 8006, Australia
4Alfred Hospital, Prahran, Victoria 3181, Australia
5Royal Melbourne Hospital, Parkville, Victoria 3052, Australia
6Royal Children's Hospital, Parkville, Victoria 3052, Australia
7Box Hill Hospital, Box Hill, Victoria 3128, Australia
8Austin Hospital, Heidelberg, Melbourne, Victoria, 3084, Australia
Correspondence to: Dr JF Seymour, E-mail: John.Seymour@petermac.org
Received 30 November 2005; revised 16 February 2006; accepted 16 February 2006; published online 28 March 2006
Ovarian cryopreservation is a promising technique to preserve fertility in women with Hodgkin lymphoma (HL) treated with chemotherapy. Thus, the aim of this study was to examine harvested ovarian tissue for subclinical involvement by HL by morphology/immunohistochemistry, and to define patient and treatment factors predictive of oocyte yield. This was a retrospective analysis of 26 ovarian tissue samples harvested for cryopreservation from women with HL. Histology, immunohistochemistry and follicle density (number mm-3) was examined. Disease status and preharvest chemotherapy details were obtained on 24 patients. The median age was 22 years (range 13-29). Seven of 24 patients had infradiaphragmatic disease at time of harvest. Nine of 20 patients had received chemotherapy preharvest (ABVD (AdriamycinÒ, Bleomycin, Vinblastine and Dacarbazine)=7, other regimens=2). The seven receiving ABVD showed no difference in follicle density compared to patients not receiving treatment (n=14); (median=1555 vs 1620 mm3 P=0.97). Follicle density measurement showed no correlation with patient age (R2=0.0001, P=0.99). There was no evidence of HL involvement in the 26 samples examined (95% CI=0-11%). In conclusion, subclinical involvement of HL has not been identified in ovarian tissue, even when patients have infradiaphragmatic disease. Furthermore, the quality of tissue harvested does not appear to be adversely affected by patient's age or prior ABVD chemotherapy.
Keywords: fertility; Hodgkin lymphoma; ovarian cryopreservation
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| Print ISSN: 0007-0920 | Online ISSN: 1532-1827 | © 2006 Cancer Research UK | ||
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