Abstract
In the quest for alternative renewable energy sources, a new self-assembled hybrid configuration of cellulose-coated oil-in-water emulsion particles with yeast was formed. In this research, the addition of yeasts (S. cerevisiae) to the micro-particle emulsion revealed a novel self-assembly configuration in which the yeast cell is connected to surrounding cellulose-coated micro-particles. This hybrid configuration may enhance the simultaneous saccharification and fermentation process by substrate channeling. Glucose produced by hydrolysis of the cellulose shells coating the micro-particles, catalyzed by cellulytic enzymes attached to their coating, is directly fermented to ethanol by the yeasts to which the particles are connected. The results indicate ethanol yield of 62%, based on the cellulose content of the emulsion, achieved by the yeast/micro-particle hybrids. The functionality of this hybrid configuration is expected to serve as a micro-reactor for a cascade of biochemical reactions in a “one-pot” consolidated process transforming cellulose to valuable chemicals, such as biodiesel.
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Introduction
Ethanol (bioethanol) made from lignocellulosic biomass holds great potential for reductions of environmental pollution compared to fossil fuels. Current methods of producing bioethanol are mainly by fermentation of glucose obtained from enzymatic hydrolysis of cellulose. Over the years, there has been significant development to improve the enzymatic hydrolysis of cellulosic materials1,2. Enhanced enzymatic hydrolysis yield can be achieved by suitable pre-treatment, such as using aqueous alkali3 or low cost protic ionic liquids (PIL)4. Moreover, a previous study has shown that the enzymatic hydrolysis rate of cellulose-coated emulsion particles, fabricated by homogenization of hydrocarbons with a suspension of regenerated cellulose hydrogel particles, exhibits similar to that of the cellulose hydrogel suspension, both being significantly enhanced compared to hydrolysis of micro-crystalline cellulose. The rate of enzymatic hydrolysis of the cellulose shell encapsulating the emulsion particles was sensitive to the cellulose/oil ratio of the particles' composition5.
Simultaneous saccharification and fermentation (SSF) has been proposed as a productive approach to improve enzymatic hydrolysis and to enhance ethanol yield6. In separate hydrolysis and fermentation processes (SHF) each reaction can take place at optimum conditions for the enzyme and the yeast. However, the enzyme is inhibited when the products, glucose and cellobiose accumulate. On the other hand, in the SSF process there is demand to compromise the optimum conditions for hydrolysis and fermentation simultaneously6,7,8,9. Furthermore, the effect of both ethanol and yeast on cellulase activity and hydrolysis of crystalline cellulose was investigated in the last decade10. Ethanol in the concentration range between 1 and 7% inhibits the enzymatic hydrolysis of cellulosic materials. However, the yeast has no direct influence on the enzymatic activity11. Wang et al. have shown that the combination of novel pretreatment method with engineered yeast can significantly improve ethanol yields from cellulosic biomass feedstocks12,13.
Emulsified SSF process with cellulose-coated emulsion micro-particles, at various suitable conditions (a variety of yeasts and temperatures) has recently been investigated as a method for rapid and efficient ethanol production. This emulsified SSF process achieved high ethanol yields (close to the theoretical cellulosic ethanol yields) by increasing the saccharification rate at temperatures tolerable to the yeast (30 °C)14. There is currently a significant effort in metabolic engineering of yeasts to achieve multi-enzyme transformation of cellulose to bio-fuel15,16,17,18. Alternatively, hybridization of the yeasts with cellulosic substrate incorporated with cellulytic enzymes, may achieve this effect without metabolic engineering. Due to the significant importance of the various implementations of yeast fermentation, several groups studied methods to improve production of ethanol by yeast fermentation such as, immobilization of yeast cells19,20,21,22,23,24,25,26,27,28,29,30,31,32,33. A study on the effect of using immobilized S. cerevisiae on bacterial cellulose (BC) for ethanol production showed that BC may be a suitable substrate for yeast immobilization due to its unique properties: high adsorption capacity, porosity, and large surface area30,34. Liu et al. have engineered S. cerevisiae with four different heterologous expressions of cellulases for application in the saccharification and fermentation process for biofuel production. They achieved a direct strategy to produce ethanol from rice straw by increasing the interactions of the engineered yeast cells with cellulose28.
Herein, we establish and examine a unique process, hybridized simultaneous saccharification and fermentation (hSSF), in which the yeasts are hybridized by self-assembly with emulsion micro-particle encapsulated by a cellulose shell. In this manner, the yeast-micro-particle hybrid provides substrate channeling in a multi-enzyme consolidated bioprocess35. We foresee that integrating the micro-particles of this hybrid system with cellulase, and lipase, the yeast-micro-particle hybrid can further function as a micro-bioreactor for a "one-pot" process transforming cellulose to valuable chemicals, such as biodiesel.
Results
Self-assembly of yeast cells and cellulose-coated o/w emulsion particles
The unique self-assembly of yeasts (S. cerevisiae) with oil in water (o/w) micro-particles, emulsified by encapsulation with unmodified cellulose, was examined using castor oil as the hydrophobic phase and coated at cellulose:oil weight ratios of 1:1 and 1:6. Cryo-SEM imaging of the fractured surface of specimens was used to characterize the structure of the hybrid system of cellulose-coated castor oil emulsion particles integrated with 1 wt.% yeast dispersion. Cryo-SEM images, after some sublimation of the surrounding frozen water, are exhibited in Figs. 1 and 2. The images reveal emulsion particles with a circular shape with clear visual borders and yeast cells which are surrounded by the cellulose-coated emulsion particles. Imaging contrast using ESB (energy-selective back scattered electrons) detector, based on the elemental composition of the specimen, allows us to distinguish between the emulsion particles (darker areas) and yeast cell (brighter areas) (Fig. 1b). Direct interaction between the cellulose-coated emulsion particles and the yeast cell is observed in Figs. 1 and 2. This adhesion seems to occur by formation of fibrils connecting the yeast cell wall with the cellulose shell of the emulsion particles (Fig. 2d). The external shell of the cellulose-coated emulsion particles contains cellulose hydrogel while the yeast cell wall includes polysaccharides β-glucans and chitin among other components36,37,38,39. The adhesion between the emulsion particles and yeast cell, as shown in Figs. 1 and 2, may be explained by direct interaction between the cellulose hydrogel and the components of the yeast cell wall. Figure 2a exhibits an intact portion of an entrapped yeast cell with cellulose-coated emulsion particles. In other cases, such as shown in Fig. 2b, the yeast cell wall was removed during fracture of the frozen samples. The inner structure of the yeast cell is clearly observed in Fig. 2c and d. The particles’ diameter ranges from 0.1 to 1 µm, while the yeast cell dimensions range from 3 to 6 µm. The particle dimensions evaluated by cryo-SEM are in accord with those observed in light and fluorescence microscope images (Figures S1 and S2, respectively). Moreover, light scattering measurements performed on hybridized system of cellulose-coated o/w emulsion with castor oil in the core with 1 wt.% yeast dispersion as well as the yeast dispersion alone, (Figure S3) exhibit good correlation with the cryo-SEM images.
Hybridized simultaneous saccharification and fermentation
This innovative system of yeast cells hybridized with cellulose-coated o/w emulsion particles fulfills a micro-environment for an effective hSSF process. It combines enzymatic hydrolysis of the aqueous cellulose hydrogel shell followed by fermentation of the released glucose in close proximity with yeast cells on micron-scale dimensions. The hSSF process to produce ethanol was monitored throughout 96 h and analyzed using GC. Figure 3 demonstrates ethanol production of 1.6 g/L. Calculation of the ethanol yield after 96 h of hSSF process provide 62% of the theoretical yield based on the weight of cellulose in the emulsion particles. The ethanol yield achieved compares favorably with other reports16.
Discussion
Integration of cellulose-coated o/w emulsion with yeast cell expose a unique self-assembly configuration: hybrids of yeast cells connected by fibrils to surrounding micro-particles. Cryo-SEM imaging provides evidence of this configuration. It exhibits direct interaction of emulsion particles and yeast cell. In this research, we demonstrate, surprisingly, that the cellulose-coated emulsion-yeast cell adhesion occurs spontaneously in the emulsion-yeast system, whereas most previous works have reported on various treatments for immobilization of yeast in cellulose40,41,42. It was found that immobilization of yeast in a cellulose matrix improved the cellulose adsorption capacity for separation application43,44,45. Moreover, this system is stable and active for an effective SSF process. The adhesion between the components of this system may enhance the SSF process by minimizing ethanol dilution into the surrounding aqueous medium, while removing the hydrolysis product from the active enzymes. However, a direct effect of the emulsion particle and yeast structure on the enzyme activity was not observed, in accord with a previous work11. This study is a proof-of-concept of the hSSF process and its viability as an effective bioreactor. It is the first work (to our knowledge) that reports on the direct interaction between a cellulose shell of cellulose-coated emulsion particles and the yeast cell wall, by fibrils that connect between them. Further studies are also needed on the formation of the fibrillary connection between the yeasts’ cell wall and the micro-particles’ cellulose shell, its biological origin and structure, and optimization of its function in substrate channeling.
Implementation of an aqueous-organic two-phase system may be an enhanced solution for the impediments of cellulosic biofuel production. In particular, for significantly improved product separation. A scheme of the one-pot process of transformation of cellulose to fatty-acid ethanol ester (FAEE, biodiesel) using cellulose-coated o/w emulsion particles as bio-reactors is shown in Fig. 4. It presents an effective cascade of biochemical processes of cellulose hydrolysis, glucose fermentation and ethanol-triglyceride trans-esterification, by hybridization of micro-organisms with cellulose-coated micro-particles incorporated with enzymes (cellulytic enzymes and lipase). The unique fibril-mediated adhesion of micro-particles to the micro-organism cell wall may enhance the fermentation and transesterification processes: glucose production at the emulsion particle surface by enzymatic cellulose hydrolysis, fermented to ethanol by the hybridized yeast. The released ethanol should be captured by the surrounding emulsion particles for subsequent transesterification with oil at their core, catalyzed by lipase, assembled at the inner interface between the core oil and the regenerated cellulose shell, to form FAEE. Despite the hybridization, a possible limitation of the hSSF process is that the ethanol yield may be decreased by loss of glucose to the surrounding aqueous medium due to incomplete substrate channeling or un-hybridized micro-particles or yeasts. These issues will be optimized in future studies.
A possible extension of this work is envisioned in conjunction with our previous study on transesterification of castor oil in the core of these emulsion micro-particles with ethanol dissolved in the aqueous medium, catalyzed by lipase within the particles. In this case, the FAEE remained dissolved in the core of the emulsion particles46. The self-assembly of cellulose-coated emulsion particles around yeast cells offers close contact on micron-scale dimensions between all components, which is expected to facilitate mass transfer of products and reactants with negligible loss to the surrounding medium. This configuration offers a promising design of an enhanced cascade of environmentally friendly biochemical processes for transformation of cellulose to biodiesel.
Experimental
Materials
Micro-crystalline cellulose powder with a particle size in the range 20–160 µm (degree of polymerization ~295 as given by the supplier) was obtained from Sigma Aldrich (Rehovot, Israel). Sodium hydroxide, 1-butanol, yeast (Saccharomyces cerevisiae) and β-Glucosidase from Aspergillus niger were purchased from Sigma Aldrich (Rehovot, Israel). Castor oil was obtained from Chen Samuel chemicals (Haifa, Israel). Celluclast 1.5 L was Purchased from Novozymes A/S (Bagsvaerd, Denmark).
Methods
Emulsion preparation and hybridization with yeast
Molecularly dissolved cellulose solutions (4 wt.%) were obtained by mixing micro-crystalline cellulose in aqueous NaOH (7 wt.%) at room temperature and then in a cooling bath (− 16 °C) using a mechanical stirrer at 500 rpm, until no crystalline cellulose was observed visually. Hydrogel dispersion was achieved by regeneration from its precursor solution by adding deionized water, until electrical conductivity measurements indicated removal of alkali traces in the hydrogel dispersion (below 1 mS cm-1).
Emulsions of cellulose-coated castor oil (Castor oil served as a model oil due to its benefits, as it is non-toxic, miscible in alcohol, renewable, biodegradable and in particular being a non-edible oil46 micro-particles, containing 1 wt.% cellulose, were obtained by two stages. First, a pre-emulsion was prepared by mixing cellulose hydrogel dispersion (~ 2.5 wt.%), castor oil (at the specified cellulose: oil ratios) and water, using a mechanical homogenizer IKA® T-18 Ultra-Turrax® (IKA Works Inc., USA) at 20,000 rpm for 5 min. The coarse emulsion was subjected to high-pressure homogenization (HPH) using a microfluidizer Model LM-20 (Microfluidics, Westwood MA, USA) at homogenization pressure of 10,000 psi for 4 min. During HPH the temperature was kept around \(40^\circ{\rm C}\) by using ice. For ensuing hybridization with the emulsion micro-particles by self-assembly, yeasts S. cerevisiae) were added to 10 ml emulsion samples, to achieve a 1 wt.% concentration.
Imaging by cryogenic scanning electron microscopy (cryo-SEM)
The morphology of cellulose-coated o/w emulsion particles was investigated using a Zeiss Ultra Plus (Carl Zeiss, Jena, Germany) high-resolution cryogenic scanning electron microscopy (cryo-HRSEM) with a Schottky field-emission gun. The cryo-HRSEM is equipped with Bal-Tec VCT100 (Balzers, Liechtenstein) cold stage maintained at temperatures below − 145 °C. The cryo-specimen preparation included placing an emulsion droplet on a stub which was vitrified by plunging into supercooled liquid ethane and then into liquid nitrogen. Then the frozen sample was transferred to a BAF060 freeze fracture unit (Leica Microsystems, Wetzlar, Germany) via a pumped cryo-transfer shuttle, maintained at − 170 °C. A rapidly cooled knife was used to fracture the frozen sample. The fractured sample was transferred to the HRSEM for imaging. Temperature was raised to − 100 °C for 30 s to remove some of the ice by sublimation to expose structural features and improve contrast47. The samples were imaged at low electron acceleration voltage (1–1.4 kV) and working distance (3–4.5 mm) to minimize charging. The Everhart–Thornley detector (SE2) and in-the-column (InLens) for high-resolution surface information was used. In addition, elemental contrast was observed in the energy-selective backscattered (“EsB”) detector. The images were examined with imageJ software, scientific image analysis software (U. S. National Institutes of Health, Bethesda, Maryland, USA).
Hybridized simultaneous saccharification and fermentation (hSSF)
hSSF was performed on hybridized emulsions of 1:6 cellulose:oil wt. ratio Celluclast (30 FPU g-1 cellulose), β-glucosidase (30 CBU g-1 cellulose) and 5 ml of sodium acetate buffer solution at a pH of 4.8 (50 mmol L-1) were added to 15 ml falcon tubes containing 5 ml hybridized emulsion sample. The SSF was carried in a shaker incubator at 40 °C, 120 rpm for 96 h. Samples for gas chromatography (GC) analysis were collected after centrifugation of 5 min at 6000 rpm. All samples were tested in four duplicates.
Reaction analysis by gas chromatography (GC)
Ethanol concentrations in the reacted SSF samples were determined using GC analysis. It was based on the standard method for quantitative determination of ethanol, but the internal standard that was used in this study is just butanol48. The samples were centrifuged for 5 min at 6000 rpm, 6 ml from the clear solution and 100 µl of butanol were transferred into a calibration bottle. Finally, 600 µl of the sample were transferred into a glass vial and injected into a CP-3800 Gas Chromatograph (Varian Inc. Walnut Creek, CA, USA) equipped with a CP-8907 column and a flame ionization detector. Flow rate of 1.1 ml min-1 was used. Calculation of the theoretical ethanol yield was done using Equation 2 proposed by Hoffman et al.14.
Data availability
All data generated or analyzed during this study are included in this published article and its supplementary information file.
References
Hu, J. et al. The accessible cellulose surface influences cellulase synergism during the hydrolysis of lignocellulosic substrates. Chemsuschem 8, 901–907 (2015).
Schülein, M. Enzymatic properties of cellulases from Humicola insolens. J. Biotechnol. 57, 71–81 (1997).
Ling, Z., Chen, S., Zhang, X. & Xu, F. Bioresource Technology Exploring crystalline-structural variations of cellulose during alkaline pretreatment for enhanced enzymatic hydrolysis. Bioresour. Technol. 224, 611–617 (2017).
Brandt-Talbot, A. et al. An economically viable ionic liquid for the fractionation of lignocellulosic biomass. Green Chem. 19, 3078–3102 (2017).
Alfassi, G., Rein, D. M. & Cohen, Y. Cellulose emulsions and their hydrolysis. J. Chem. Technol. Biotechnol. 94, 178–184 (2019).
Öhgren, K., Bura, R., Lesnicki, G., Saddler, J. & Zacchi, G. A comparison between simultaneous saccharification and fermentation and separate hydrolysis and fermentation using steam-pretreated corn stover. Process Biochem. 42, 834–839 (2007).
Wingren, A., Galbe, M. & Zacchi, G. Techno-economic evaluation of producing ethanol from softwood: Comparison of SSF and SHF and identification of bottlenecks. Biotechnol. Prog. 19, 1109–1117 (2003).
Szambelan, K., Nowak, J., Szwengiel, A., Jeleń, H. & Łukaszewski, G. Separate hydrolysis and fermentation and simultaneous saccharification and fermentation methods in bioethanol production and formation of volatile by-products from selected corn cultivars. Ind. Crops Prod. 118, 355–361 (2018).
Hari Krishna, S., Janardhan Reddy, T. & Chowdary, G. V. Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast. Bioresour. Technol. 77, 193–196 (2001).
Biol, E., Douro, A. & Real, V. Enzymatic kinetic of cellulose hydrolysis. 126, (2005).
Chen, H. & Jin, S. Effect of ethanol and yeast on cellulase activity and hydrolysis of crystalline cellulose. Enzyme Microb. Technol. 39, 1430–1432 (2006).
Wang, Z., Dien, B. S., Rausch, K. D., Tumbleson, M. E. & Singh, V. Improving ethanol yields with deacetylated and two-stage pretreated corn stover and sugarcane bagasse by blending commercial xylose-fermenting and wild type Saccharomyces yeast. Bioresour. Technol. 282, 103–109 (2019).
Sun, J. et al. One-pot integrated biofuel production using low-cost biocompatible protic ionic liquids. Green Chem. 19, 3152–3163 (2017).
Hoffman, S. M., Alvarez, M., Alfassi, G., Rein, D. M. & Echauri, S. G. Biotechnology for Biofuels Cellulosic biofuel production using emulsified simultaneous saccharification and fermentation (eSSF) with conventional and thermotolerant yeasts. Biotechnol. Biofuels https://doi.org/10.1186/s13068-021-02008-7 (2021).
Madhavan, A. et al. Bioresource technology design and genome engineering of microbial cell factories for efficient conversion of lignocellulose to fuel. Bioresour. Technol. 370, 128555 (2023).
Bamba, T., Guirimand, G., Kondo, A. & Hasunuma, T. ScienceDirect enzyme display technology for lignocellulosic biomass valorization by yeast cell factories. Curr. Opin. Green Sustain. Chem. 33, 100584 (2022).
Zhang, C., Chen H., Zhu Y., Zhang Y., Li X. & Wang F. Saccharomyces cerevisiae cell surface display technology: Strategies for improvement and applications. 1–15 (2022) doi:https://doi.org/10.3389/fbioe.2022.1056804.
Vu, V. N. H. et al. Design and construction of artificial microbial consortia to enhance lignocellulosic biomass degradation. Biofuel Res. J. 39, 1890–1900 (2023).
Żywicka, A., Banach, A., Junka, A. F., Drozd, R. & Fijałkowski, K. Bacterial cellulose as a support for yeast immobilization–correlation between carrier properties and process efficiency. J. Biotechnol. 291, 1–6 (2019).
Winkelhausen, E., Velickova, E., Amartey, S. A. & Kuzmanova, S. Ethanol production using immobilized saccharomyces cerevisiae in lyophilized cellulose gel. Appl. Biochem. Biotechnol. 162, 2214–2220 (2010).
Eiadpum, A., Limtong, S. & Phisalaphong, M. High-temperature ethanol fermentation by immobilized coculture of Kluyveromyces marxianus and Saccharomyces cerevisiae. J. Biosci. Bioeng. 114, 325–329 (2012).
Nuanpeng, S., Thanonkeo, S., Klanrit, P. & Thanonkeo, P. Ethanol production from sweet sorghum by Saccharomyces cerevisiae DBKKUY-53 immobilized on alginate-loofah matrices. Brazilian J. Microbiol. 49, 140–150 (2018).
Wen, F., Sun, J. & Zhao, H. Yeast surface display of trifunctional minicellulosomes for simultaneous Saccharification and fermentation of cellulose to ethanol. Appl. Environ. Microbiol. 76, 1251–1260 (2010).
Erkan, S. B., Yatmaz, E., Germec, M. & Turhan, I. Effect of furfural concentration on ethanol production using Saccharomyces cerevisiae in an immobilized cells stirred-tank bioreactor with glucose-based medium and mathematical modeling. J. Food Process. Preserv. 45, 1–12 (2021).
Verbelen, P. J., De Schutter, D. P., Delvaux, F., Verstrepen, K. J. & Delvaux, F. R. Immobilized yeast cell systems for continuous fermentation applications. Biotechnol. Lett. 28, 1515–1525 (2006).
Najafpour, G., Younesi, H. & Ku Ismail, K. S. Ethanol fermentation in an immobilized cell reactor using Saccharomyces cerevisiae. Bioresour. Technol. 92, 251–260 (2004).
El-Dalatony, M. M. et al. Long-term production of bioethanol in repeated-batch fermentation of microalgal biomass using immobilized Saccharomyces cerevisiae. Bioresour. Technol. 219, 98–105 (2016).
Liu, Z. et al. Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production. Sci. Rep. 6, 1–7 (2016).
Orrego, D., Zapata-Zapata, A. D. & Kim, D. Ethanol production from coffee mucilage fermentation by S. cerevisiae immobilized in calcium-alginate beads. Bioresour. Technol. Reports 3, 200–204 (2018).
Khanpanuek, S., Lunprom, S., Reungsang, A. & Salakkam, A. Repeated-batch simultaneous saccharification and fermentation of cassava pulp for ethanol production using amylases and Saccharomyces cerevisiae immobilized on bacterial cellulose. Biochem. Eng. J. 177, 108258 (2022).
Karagoz, P., Bill, R. M. & Ozkan, M. Lignocellulosic ethanol production: Evaluation of new approaches, cell immobilization and reactor configurations. Renew. Energy 143, 741–752 (2019).
Singh, A., Sharma, P., Saran, A. K., Singh, N. & Bishnoi, N. R. Comparative study on ethanol production from pretreated sugarcane bagasse using immobilized Saccharomyces cerevisiae on various matrices. Renew. Energy 50, 488–493 (2013).
Yao, W. et al. Bacterial cellulose membrane–a new support carrier for yeast immobilization for ethanol fermentation. Process Biochem. 46, 2054–2058 (2011).
Chen, C. & Hu, L. Nanocellulose toward advanced energy storage devices: Structure and electrochemistry. Acc. Chem. Res. 51, 3154–3165 (2018).
Kummer, M. J. et al. Substrate channeling by a rationally designed fusion protein in a biocatalytic cascade. JACS Au https://doi.org/10.1021/jacsau.1c00180 (2021).
Osumi, M. The ultrastructure of yeast: Cell wall structure and formation. Micron 29, 207–233 (1998).
Anwar, M. I., Muhammad, F., Awais, M. M. & Akhtar, M. A review of ß-glucans as a growth promoter and antibiotic alternative against enteric pathogens in poultry. Worlds. Poult. Sci. J. 73, 651–661 (2017).
Aguilar-Uscanga, B. & François, J. M. A study of the yeast cell wall composition and structure in response to growth conditions and mode of cultivation. Lett. Appl. Microbiol. 37, 268–274 (2003).
Dallies, N., François, J. & Paquet, V. A new method for quantitative determination of polysaccharides in the yeast cell wall. Application to the cell wall defective mutants of Saccharomyces cerevisiae. Yeast 14, 1297–1306 (1998).
Żywicka, A., Peitler, D., Rakoczy, R., Junka, A. F. & Fijałkowski, K. Wet and dry forms of bacterial cellulose synthetized by different strains of gluconacetobacter xylinus as carriers for yeast immobilization. Appl. Biochem. Biotechnol. 180, 805–816 (2016).
Ivanova, V., Petrova, P. & Hristov, J. Application in the ethanol fermentation of immobilized yeast cells in matrix of alginate/magnetic nanoparticles, on chitosan-magnetite microparticles and cellulose-coated magnetic nanoparticles. 3, 289–299 (2011).
Żywicka, A. et al. Immobilization pattern of morphologically different microorganisms on bacterial cellulose membranes. World J. Microbiol. Biotechnol. 35, 1–11 (2019).
Arunraj, B., Sathvika, T., Rajesh, V. & Rajesh, N. Cellulose and saccharomyces cerevisiae embark to recover europium from phosphor powder. ACS Omega 4, 940–952 (2019).
Sathvika, T., Rajesh, V. & Rajesh, N. Microwave assisted immobilization of yeast in cellulose biopolymer as a green adsorbent for the sequestration of chromium. Chem. Eng. J. 279, 38–46 (2015).
Vasconcelos, N. F. et al. Oxidized bacterial cellulose membrane as support for enzyme immobilization: properties and morphological features. Cellulose 27, 3055–3083 (2020).
Hamal, E. K., Alfassi, G., Rein, D. M. & Cohen, Y. Towards one-pot consolidated bioprocessing of cellulose to biodiesel: Lipase-catalyzed transesteri fi cation at cellulose-coated oil-in-water emulsions as micro-reactors. J. Chem. Technol. Biotechnol. https://doi.org/10.1002/jctb.7132 (2022).
Koifman, N. & Talmon, Y. Cryogenic electron microscopy methodologies as analytical tools for the study of self-assembled pharmaceutics. Pharmaceutics 13, 1015 (2021).
Hunter, R. A. Analysis of ethanol in beer using gas chromatography: A side-by-side comparison of calibration methods. J. Chem. Educ. 98, 1404–1409 (2021).
Acknowledgements
This project was funded by the Israel Science Foundation Grant no. 449/23, and by the Russel Berrie Nanotechnology Institute for equipment use. SEM specimen preparation and imaging were performed at the Technion Center for Electron Microscopy of Soft Matter. The authors thank N. Koifman for her assistance in cryo-electron microscopy imaging. Fluorescence microscopy was performed at the Life sciences and Engineering Infrastructure center at the Technion. The authors thank Y. Lupu-Haber for her assistance in fluorescence microscopy imaging.
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E.K.H, Y.C, G.A and D.M.R developed the concept and experimental design. E.K.H conducted the experiments, analyzed the results and prepared the manuscript. G.A performed the GC measurements. M.A contributed to the experimental design and preliminary experiments. G.A, D.M.R and Y.C supervised the research. All authors have discussed, reviewed and approved the manuscript.
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Hamal, E.K., Alfassi, G., Antonenko, M. et al. Cellulose-coated emulsion micro-particles self-assemble with yeasts for cellulose bio-conversion. Sci Rep 14, 5499 (2024). https://doi.org/10.1038/s41598-024-56204-0
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DOI: https://doi.org/10.1038/s41598-024-56204-0
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