Correction to: Scientific Reports https://doi.org/10.1038/s41598-023-42037-w, published online 16 September 2023

The original HTML version of this Article contained errors in Figures 1 to 5, where the dendrograms were not displayed correctly.

The original Figures 1, 2, 3, 4 and 5 and their accompanying legends appear below.

Figure 1
figure 1

Genome-wide DNA methylation analysis of CTC-MCC-41 with respect to HT29 primary tumor cells. (A) Schematic flowchart used to identify significant differentially methylated CpGs in CTC-MCC-41 compared to HT29. (B) Principal component analysis (PCA) of DNA methylation data obtained in CTC-MCC-41 and HT29 cells. (C) Scatter plot representing the mean normalized levels of DNA methylation (β-values) in CTC-MCC-41 and HT29 cells. Dots in red show significantly differentially methylated CpGs. (D) Hierarchical clustering heatmap of the 10,000 most differentially methylated CpGs (FDR adjusted p value < 0.05) between CTC-MCC-41 and HT29. Heatmap shows three different passages (P) of CTC-MCC-41 (P12, P13 and P14) and HT29 (P2, P3 and P4). (E, F) Description of the 188,185 differentially methylated CpGs (DMCpGs) found in CTC-MCC-41 with respect to HT29 according to (E) chromosome location and methylation status and (F) CpG context, gene location and transcription factor-binding sites (TFBS). QC quality control, FDR false discovery rate, CpGI CpG island, HypoM hypomethylated, HyperM hypermethylated.

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Figure 2
figure 2

DNA methylation profiles of CpGIs and shore regions of gene promoters in CTC-MCC-41 compared to HT29 primary tumor cells. (A) Hierarchical clustering heatmap of the 10,000 most differentially methylated CpGs (FDR adjusted p value < 0.05) in CTC-MCC-41 with respect to HT29 and located at CpGIs and shore regions of gene promoters. Heatmap shows three different passages (P) of CTC-MCC-41 (P12, P13 and P14) and HT29 (P2, P3 and P4). (B) Gene Ontology (GO) analysis representing some of the most cancer-relevant biological processes and Panther pathways based on the 10,000 most differentially methylated CpGs of CTC-MCC-41 compared to HT29 and located at CpGIs and shore regions of gene promoters.

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Figure 3
figure 3

Genome-wide DNA methylation analysis of CTC-MCC-41 with respect to COLO205 metastatic tumor cells. (A) Schematic flowchart used to identify significantly differentially methylated CpGs in CTC-MCC-41 compared to COLO205. (B) Principal component analysis (PCA) of DNA methylation data obtained for CTC-MCC-41 and COLO205 cells. (C) Scatter plot representing the mean normalized levels of DNA methylation (β-values) in CTC-MCC-41 and COLO205 cells. Dots in red show significantly differentially methylated CpGs. (D) Hierarchical clustering heatmap of the 10,000 most differentially methylated CpGs (FDR adjusted p value < 0.05) between CTC-MCC-41 and COLO205. Heatmap shows three different passages (P) of CTC-MCC-41 (P12, P13 and P14) and COLO205 (P2, P3 and P4). (E, F) Description of the 188,185 differentially methylated CpGs (DMCpGs) found in CTC-MCC-41 with respect to COLO205 according to (E) chromosome location and methylation status and (F) CpG context, gene location and transcription factor-binding sites (TFBS). QC quality control, FDR false discovery rate, CpGI CpG island, HypoM hypomethylated, HyperM hypermethylated.

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Figure 4
figure 4

DNA methylation patterns of CpGIs and shore regions of gene promoters in CTC-MCC-41 compared to COLO205 metastatic tumor cells. (A) Hierarchical clustering heatmap of the 10,000 most differentially methylated CpGs (FDR adjusted p value < 0.05) in CTC-MCC-41 with respect to COLO205 and located at CpGIs and shore regions of gene promoters. Heatmap shows three different passages (P) of CTC-MCC-41 (P12, P13 and P14) and COLO205 (P2, P3 and P4). (B) Gene Ontology (GO) analysis representing some of the most cancer-relevant biological processes and Panther pathways based on the 10,000 most differentially methylated CpGs of CTC-MCC-41 compared to COLO205 and located at CpGIs and shore regions of gene promoters.

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Figure 5
figure 5

DNA methylation signature of CTC-MCC-41 with respect to colorectal primary and metastatic tumor cells. (A) Hierarchical clustering heatmap with the 17,827 differentially methylated CpGs (FDR adjusted p value < 0.05) in CTC-MCC-41 cells compared to HT29 and COLO205 cells, representing primary and metastatic tumor cells, respectively. (B) Hierarchical clustering heatmap of the 9,949 differentially methylated CpGs (FDR adjusted p value < 0.05) in CTC-MCC-41 compared to several colorectal primary (HT29, Caco2, HCT116, RKO) and metastatic tumor cells (COLO205 and SW620). Heatmaps show three different passages (P) of CTC-MCC-41 (P12, P13 and P14), HT29 (P2, P3 and P4), and COLO205 (P2, P3 and P4).

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These errors have now been corrected in the HTML version of the Article; the PDF version was correct from the time of publication.