Abstract
Ocean acidification can negatively impact the early life-stages of marine fish, due to energetic costs incurred by the maintenance of acid–base homeostasis, leaving less energy available for growth and development. The embryos of intertidally spawning fishes, such as Pacific herring, are often air exposed for hours. We hypothesized that air exposure would be beneficial to the developing embryo due to a higher oxygen availability (and thus reduced metabolic costs to secure adequate oxygen) and permitting excess CO2 associated with ocean acidification to be off-gassed during emersion. To investigate this, we reared Pacific herring (Clupea pallasii) embryos under three tidal regimes (subtidal: fully immersed, low intertidal: 2 × 2 h air exposure, and high intertidal: 5 + 9 h air exposure) fully crossed with three aquatic CO2 levels (400, 1500 and 3200 µatm) at a water temperature of 9.5 °C and naturally fluctuating air temperature during air exposure. We measured the effects on embryonic development and hatch, as well as carry-over effects on larval development and survival. Air exposure during embryonic development had significant positive effects on growth, condition and survival in larval Pacific herring, with some interactive effects with CO2. Interestingly, CO2 by itself in the fully immersed treatment had no effect, but had significant interactions with air exposure. Our research suggests that air exposure during low tide can be highly beneficial to intertidally spawning fishes and needs to be taken into account in climate change studies and modeling.
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Introduction
The eastern North Pacific is an ocean region particularly impacted by climate drivers, where high CO2 levels shoaling to the surface with upwelling are an important feature of the ocean dynamics1,2,3. Pacific herring (Clupea pallasii) are a keystone species in the North Pacific as an important forage fish in the marine food web4. Being one of the main prey items of Pacific salmon, which in turn are the main prey of resident orcas (Orcinus orca), they play a key role in the ecosystem, economy and culture in the Pacific Northwest4. Pacific herring stocks have shown substantial fluctuations in the last two decades, and some management units are at all time low levels, with direct and indirect effects of climate change likely contributing to their decline5,6,7. Year-class strength is determined by survival of the vulnerable larval stage, which in turn is strongly dependent on environmental factors affecting successful development8. One such environmental factor is ocean acidification (OA), to which the NE Pacific may be particularly vulnerable due to naturally high concentrations of aquatic CO2 from both natural and anthropogenic drivers including upwelling, low buffering capacity, riverine input and eutrophication9,10,11,12,13,14. Detrimental effects of future projections of ocean acidification levels have been documented in experiments with embryonic and larval Pacific and Atlantic herring (Clupea harengus), with lowered condition factor, developmental delays and malformations reported at CO2 levels as low as 1000 µatm15,16,17,18, levels which already occur episodically in coastal regions including the Salish Sea19,20,21,22. These effects are likely a result of increased energetic costs associated with maintaining acid–base homeostasis in elevated environmental CO223,24.
However, Pacific herring are intertidally spawning fishes and as a result, the embryos are periodically exposed to air during low-tide, in which gas exchange of oxygen and CO2 between the embryo and the atmosphere occurs. While the physiological effects of tidally fluctuating CO2 levels have been studied to some extent in larval fish25, the effects of air exposure have not been included. We propose that air exposure during embryogenesis may play an important role in counteracting negative effects of high marine CO2 levels in the wild.
Pacific herring spawn in winter in the intertidal and upper sub-tidal zones, synchronized with the tides, on marine vegetation in sheltered regions and estuaries. Spawning height is dependent on tidal height and beach slope26, and also female choice with larger females tending to deposit larger eggs higher in the intertidal as a form of parental care where the embryos are exposed to air for several hours per day27. Tide pools and estuaries can become hypoxic at low tide, particularly at night, and thus fishes that spawn in air exposed regions during low tide avoid hypoxic conditions for their embryos. The greater oxygen availability and diffusivity in air can be further advantageous to embryos, by accelerating development at reduced metabolic costs28. Air-reared embryos of the mangrove killifish, Kryptolebias marmoratus, consumed nearly half the amount of oxygen and had larger yolk reserves compared to water-reared embryos while maintaining comparable growth rates, indicating highly reduced energetic costs of development in air29. In addition to a higher oxygen availability, air exposure may allow the embryos to off-gas excess CO2, thus temporarily alleviating some of the negative effects of ocean acidification. While air exposure has been found to increase mortality in Pacific herring27 and capelin (Mallotus villosus)30, this has mainly been ascribed to desiccation and temperature stress. For example, in Pacific herring, air exposure had no effect on survival in embryos at 5 and 8 °C, with a mean 80% survival, while 5–8 h daily air exposure at 11 °C decreased survival to as low as 5%31. For Pacific herring spawning on the coast of British Columbia (BC) in winter, high temperatures and desiccation may not be a common problem given the seasonal cold temperatures (typically 0–10 °C) and high humidity (typically 40–100%) that they experience at that time of year32.
In this study, we hypothesized that air exposure during embryonic development of Pacific herring would reduce negative effects of ocean acidification on embryonic development and hatch success, with carry-over effects on larval growth, development and survival. To address this, fertilized herring eggs were collected from the wild and brought into the lab where they were exposed to one of three CO2 treatments (1) 400 µatm, (2) 1600 µatm and (3) 3000 µatm, designed to mimic control conditions, medium CO2 conditions present during winter mixing and high CO2 levels predicted with climate change, respectively. These three CO2 treatments were fully crossed with one of three air exposure treatments during embryonic development (1) subtidal: fully immersed, (2) low intertidal: 2 × 2 h day−1 and (3) high intertidal: 5 h + 9 h day−1. For ecological relevance, all air exposure treatments were conducted in naturally fluctuating air temperature and ambient humidity. We hypothesized that (H1) CO2 would negatively affect embryonic and larval growth, survival and development, cause cranial and spinal malformations and increase heart rate; (H2) Moderate air exposure in the low intertidal (2 × 2 h air exposure day−1) would be beneficial to the embryos improving growth and survival and counteract the negative effects of elevated CO2 described in H1; (H3) Prolonged air exposure in the high intertidal (5 h + 9 h air exposure day−1) would counteract negative effects of elevated CO2 on embryonic development and expected cranial and spinal malformations, but result in synergistic negative effects on larval growth and survival. A graphical abstract is shown in Fig. 1.
Material and methods
Egg collection and husbandry
Wild spawned herring eggs attached to algae were collected from Williams Beach, Vancouver Island, British Columbia on March 5, 2021 at 1 pm during the receding tide. Eggs were brought back to the Hakai Marine Station, Quadra Island, British Columbia in coolers where they were sorted for viability and staged to be between e and f, approximately 2.5 days post fertilization (dpf)33, with 90% epiboly but no optic vesicle or myomeres apparent. Live embryos were randomly distributed into 27 mesh hatch boxes (Pen Plax NB2 Net Breeder Deluxe; dimensions: 2.79 × 14.22 × 28.96 cm) at a density of approximately 300 eggs per hatch box. These were in turn floated in one of 9 tanks (3 hatch boxes per tank) randomly set to three different CO2 treatments in triplicate: (1) control CO2 at 400µatm, (2) medium CO2 at 1600 µatm and (3) high CO2 at 3000 µatm. The treatment tanks were 380 L glass aquaria with a 260 L holding tank in front and a 120 L back chamber which was used for chilling and CO2 equilibration. Tanks were set to partial flow-through with filtered seawater taken from 20 m depth in Hyacinth Bay, and partial recirculation with water from the back chamber. Target CO2 tensions were accomplished by equilibrating water with fine air diffusers with a pre-set CO2-air mixture using mass-flow controllers in the back chamber and circulated through the holding tank. Each tank had a individual pH and temperature meters recording values every 5 min. Water temperature was maintained at 9.5 °C with an industrial chiller coil in the back chamber, which matched the ocean temperature at which the embryos were collected. Oxygen, salinity, temperature and pH were monitored daily with handheld meters (YSI), and ammonia was monitored every other day and remained below 0.1 ppm.
Each of the 3 hatch boxes floating in each replicate tank was assigned to one of three air exposure regimes: (1) continuous immersion, (2) 2 h day and 2 h night air exposure (2 × 2 h day−1) and (3) 5 h day and 9 h night air exposure (5 h + 9 h day−1), corresponding to what herring embryos would experience in the (1) subtidal, (2) low intertidal and (3) high intertidal, resulting in a fully crossed 3 air-exposure × 3 CO2 treatments in triplicate (27 hatch boxes). During air-exposure, hatch boxes were placed into a communal shaded, dry sea table outside where they were exposed to natural temperature fluctuations throughout the day and night. Air temperature was monitored throughout. Each day, dead embryos were counted and boxes were checked for hatched larvae.
At first signs of hatch, embryos were transferred from the hatch boxes into 2 L glass jars covered with fine mesh and immersed back into the same 9 aquaria for CO2 equilibration (27 glass jars in total), in order to prevent escape, keeping each treatment and replicate separate. One day post peak hatch, algae were removed from the jars and all egg shells, dead and unhatched embryos were counted and removed. Each day, dead larvae were removed via siphon and counted. Every other day, pH, temperature, salinity, oxygen and ammonia were measured in all individual jars and 25% of the water in each jar was exchanged with the corresponding tank water. Discreet water samples were taken for carbonate chemistry measurements on day 1, 6 and 15 of the experiment.
At 5 days post hatch (dph), a mass mortality occurred in one jar of the control CO2, high intertidal (5 + 9 h air exposure) treatment, where 89% of the larvae died for reasons unknown. Oxygen, ammonia and pH levels were normal and water appeared clear. As the other two replicates were unaffected, larvae from this jar were removed from further analysis.
Sampling and analysis
Herring embryos were sampled at 6 dpf which corresponded to 4 days after transfer into treatment. Four embryos per hatch box were photographed live in a dish of water from their tanks under a dissecting microscope (Zeiss Stemi 508 with a mounted Sony A7R4) at 0.8× and 1.6× magnification for staging and morphometrics. Heart beats were video taped at 1.6× magnification for 10 s for calculation of heart rate as an indicator of metabolic rate. Water temperatures in the dishes were monitored and were consistently 12 °C. Embryos were then transferred to Eppendorf caps, frozen in liquid nitrogen and stored at − 80 °C for further analysis. Larvae were sampled at hatch (0 dph), 3 dph and 5 dph. Ten larvae per jar were photographed live under the microscope at 0.63× and 1.6× magnification for morphometrics and length measurements, transferred individually into caps, frozen in liquid nitrogen and stored at − 80 °C for further analysis. The experiment was terminated at yolk-sac depletion (6 dph) at which point all larvae were counted out and a subsample frozen at − 80 °C. Frozen herring larvae were weighed, freeze-dried overnight and weighed dry the next day on a high precision microbalance (Satoris). From the images, larval length, body depth, body area, yolk sac area, eye diameter, distance between eyes and the head length and width were measured as indicators for development (Fig. 2) using image J. Growth rates (of body area, length and weight) and yolk sac depletion rates (from yolk sac area measurements) were calculated as a measure of energy transfer of yolk into growth. Weight and length measurements were used to calculate Fulton’s condition factor K. Larval developmental stage was determined after Kawakami et al.33 for each larvae and larval images were assessed for cranial and spinal malformations using symmetry and head dimensions for cranial assessment and straightness for spinal assessment.
All analyses were done blind to the treatment from which they came and in random order.
Animal care
This study was carried out under strict accordance with the animal care protocol approved by the University of British Columbia Animal Care Committee (certificate # A19-0284). Wild embryos were collected under the Fisheries and Oceans permit number XR 57 2021.
Statistics
All statistics were done in R34 and plots were generated using ggplot. After testing for normality and homogeneity of variance, main effects and interactions of CO2 and tidal exposure were tested with linear mixed models, including hatch box or jar—henceforth known as “replicate”—as a random factor, using the lmer function from the package lme4, with the outputs of p-values and Chi square (X2). Contrasts in tidal regime across fixed CO2 levels were made using packages phia and multcomp. P-value adjustments were made using the holm method. Percent hatched and dead larvae were arcsin-transformed and percent daily embryonic mortality was ln-transformed for normal distribution and analyzed using a multifactorial ANOVA, giving p- and F-values. Effect size analyses were performed by calculating the natural logarithm of the response ratio (LnRR) of the air exposure treatments relative to the subtidal regime with 95% confidence intervals at each of the three CO2 levels35. Effects of treatment on correlation of morphometrics (body length, head width, head length, distance between eyes, eye diameter and cranial deformities) were tested with a principal component analysis using the packages devtools and factoextra.
Results
Abiotic parameters
The temperature regime experienced by the embryos was purposefully natural and therefore varied between the three air exposure treatments. The subtidal treatment, where embryos were continuously submerged in water, remained around 9.5 °C for the duration of the experiment, while the intertidal treatments experienced dips in temperature during outside air exposure down to 2.5 °C and 0.8 °C, for the low and high intertidal respectively (Fig. 3A). Accumulated thermal units (ATU; days × temperature post collection until hatch) for each air exposure treatment were 79.6, 75.4 and 65.3 for subtidal, low intertidal and high intertidal, respectively. Despite differences in thermal regime, peak hatch was on the same day (March 14, 2021) for all air exposure and CO2 treatments, estimated at 11 dpf.
The pH levels in the tanks were measured hourly and were stable over the course of the embryonic incubation period, with no overlap between treatments, although there was some overlap between individual jars. Control treatment was consistently around a pH of 8, the medium treatment had a pH of 7.4 and the high CO2 treatment had a mean pH of 7.1 (Table 1). After hatch, when the larvae were transferred to the jars, circulation and gas exchange between jars and tank were not as high and CO2 accumulated in the jars over time, leading to pH levels deviating from tank pH levels (Fig. 3B). Although oxygen levels remained high (7–9 mg/L), the pH dropped from a mean 8–7.6 in the control on two occasions, and was brought back up with a partial water exchange from the incubation tank water. The pH in the medium and high CO2 treatments were not as affected (Fig. 3B), however, final water chemistry measurements after completion of the experiment (2 days post water exchange) revealed much higher CO2 levels in all treatments (Table 1: day 15).
Effect of air exposure and CO2 treatment during embryonic development
Neither embryonic survival nor growth were significantly affected by treatment in our experiment. Percent daily embryonic mortality was low and not significantly affected by CO2 treatment or air exposure (CO2: p = 0.088, F2 = 2.45; Tide: p = 0.11, F2 = 2.19; CO2*Tide: p = 0.18, F2 = 1.59) . Egg diameter at 6 dpf was also not significantly affected by treatment (CO2: p = 0.38, X2 (2, N = 30) = 1.92; Tide: p = 0.83, X2 (2, N = 30) = 0.33; CO2*Tide: p = 0.08, X2 (2, N = 30) = 8.25). Metabolic rate, as indicated by embryonic heart rate, was significantly affected by air exposure at 6 dpf (p < 0.001, X2 (2, N = 12) = 17.38), but not CO2 (p = 0.09, X2 (2, N = 12) = 4.74 or CO2*Tide (p = 0.15, X2 (2, N = 12) = 6.66. Interestingly, in the medium CO2 treatment, embryonic heart rate was significantly lower in the high intertidal treatment compared to the low intertidal and subtidal treatment (p = 0.027, X2 (2, N = 12) = 8.6), while in the high CO2 treatment, heart rate was significantly increased by 10% in the low intertidal compared to the subtidal and high intertidal regime (p = 0.007, X2 (2, N = 12) = 12.0) (Fig. 4A, Tables 2 and 3).
Overall hatch success was very high in all treatments, and ANOVA did not show a significant affect of CO2 (p = 0.203; F2 = 1.75), air exposure (p = 0.668, F2 = 0.41) nor the interaction (p = 0.768, F4 = 0.45). Effect size analysis showed a significant positive effect of tidal exposure in the ambient CO2 treatment, with 14% higher hatch rate in the high intertidal treatment compared to the subtidal treatment (Table 3, Fig. 4B).
Figure 4C shows the effect of air exposure and CO2 on larval size at hatch. Both CO2 and tidal exposure were significant (CO2: p = 0.007, X2 (2, N = 30) = 9.8; Tide: X2 (2, N = 30) = 40.6), with length negatively correlated with air exposure. The greatest effect was seen in the medium CO2 treatment, where short air exposure lead to 2% smaller larvae in the low intertidal treatment compared to no air exposure, and long air exposure in the high intertidal treatment lead to 6% smaller larvae compared to no air exposure in the subtidal treatment (Table 3). Length at hatch was also significantly affected by CO2 treatment, with smaller larvae hatching in the medium and high CO2 treatment with prolonged air exposure compared to control CO2 conditions. For larvae reared in high CO2 conditions, only long air exposure in the high intertidal treatment had a significant effect on size at hatch with larvae 5% smaller than those with no air exposure in the subtidal treatment (for a summary of statistics, see Tables 2 and 3).
Carry-over effects on the larval phase
The greatest effect of air exposure was the reduction in cumulative larval mortality from hatch to yolk-sac depletion at 6 dph (Fig. 4D). Larval mortality was significantly affected by tidal exposure (p < 0.001, F2 = 11.21), but not CO2 (p = 0.198, F2 = 1.78) nor the interaction (p = 0.869, F4 = 0.31). Significant differences in mortality were found in all air exposed treatments relative to the subtidal, fully immersed treatment, with a 70%—80% reduction in larval mortality with prolonged air exposure in the high intertidal across CO2 treatments (Table 3).
Analyses of larval morphology, including length and height, wet and dry weight, yolk sac area and depletion rate, spinal and cranial morphology, and eye diameter revealed no significant effects of CO2 and air exposure, either alone or in combination with a pca. The daily growth rate computed for individual larvae from length at 5 dph relative to the mean length at hatch for the respective replicate, was significantly affected by air exposure (p = 0.002, X2 (2, N = 30) = 12.46), with growth rate increasing with increasing air exposure in the medium CO2 treatment (Tables 2 and 3). Interestingly, yolk sac depletion rate was not significantly affected by CO2 (p = 0.75, X2 (2, 30) = 0.57) or air exposure (p = 0.26, X2 (2, 30) = 2.71), and there was no significant interaction (p = 0.92, X2 (4, 30) = 0.96). Cranial abnormalities were found in very few the larvae and these were not correlated with treatment or other morphometric components in a pca.
Larval dry weight: wet weight was significantly affected by CO2 (p = 0.003, F2 = 1.66) and the interaction between CO2 and tide was marginally significant (p = 0.060, F4 = 2.26). In a linear mixed model relating wet weight to length, CO2 and tidal exposure, length and CO2 were significant (length: p < 0.001, X2 (1, N = 10) = 139.3; CO2: p = 0.001, X2 (2, N = 10) = 13.08) and the interaction of the three parameters was marginally significant (p = 0.08, X2 (4, N = 10) = 8.12). Figure 5 shows the relationship between wet weight and length for different CO2 and tidal treatments. The control treatment had a higher slope with moderate air exposure in the low intertidal treatment, indicating improved condition in this treatment compared to the subtidal treatment (Fig. 5). Prolonged air exposure in the high intertidal, on the other hand, significantly increased the slope and therefore improved condition in the medium and high CO2 treatments. Note that the slopes of weight-length relationships in the low intertidal control treatment and the high intertidal medium CO2 treatment are the same, indicating that prolonged air exposure improved larval condition in the medium treatment up to the levels of the control. In contrast, in the high intertidal treatment condition was improved with air exposure but not up to levels seen in the high CO2 treatment (Fig. 5).
Discussion
This study uniquely explores the role of different aquatic CO2 levels, in the presence and absence of air exposure, in the early life history of an important marine fish. We hypothesized that air exposure during embryonic development of Pacific herring would reduce negative effects of ocean acidification on embryonic development and hatch success, with positive carry-over effects on larval growth, development and survival. Contrary to our hypothesis and previously published studies on larval herring15,16,18, ocean acidification did not affect embryonic survival and development, nor cause cranial and spinal malformations in our herring larvae. Metabolic rate in embryos, implied from heart beat frequency, was elevated only in the high CO2 treatment with moderate air exposure, but not prolonged air exposure, showing no consistent rescue effect of air exposure on metabolic rate. Air exposure, however, did have strong beneficial effects on larval survival with the effects increasing with aquatic CO2 level and air exposure, implying that air exposure during embryonic development has strong carry-over effects for larval survival. Growth rates were faster in larvae where embryos had been exposed to air in the medium CO2 treatment, but not in the control or high CO2 treatment. On the other hand, larval condition, implied from weight-length ratio, decreased with high ocean acidification, but was partially restored by prolonged air exposure. Our results show that air exposure during embryonic development has strong carry-over effects on larvae and can potentially mitigate negative effects on larvae from ocean acidification.
Effect of CO2 in fully immersed embryos
None of our response parameters were affected by CO2 levels up to 3000 µatm in our fully immersed (subtidal) treatment. Survival and hatch success were very high in all treatments, between 70 and 90%. Only the medium CO2 subtidal treatment showed a large variation in hatch success, however, this was likely linked to substrate, as discussed in more detail below. CO2 exposure did not affect growth, development or yolk sac utilization, nor was it correlated with cranial or spinal abnormalities, as has been found in both Pacific and Atlantic herring larvae in response to elevated CO215,16. A recent study found that 1200 µatm CO2 in combination with higher temperature (16 °C) led to significantly increased embryonic mortality, heart rate and larger yolk sac15. However, at a comparable control temperature (10 °C), embryonic survival rates in that study were similar to ours, regardless of CO2 level. Heart rates in our study were higher than those measured by Villalobos et al.15 at the same developmental stage at 10 °C, and more closely matched those measured in their control CO2 treatment at 16 °C. It is possible that heart rates may have been elevated due to our higher water temperature under the dissecting scope (12 °C). Nevertheless, we did not see a strong signal of ocean acidification on heart beat frequency in the fully submerged treatments.
Combined effect of air exposure and CO2
Counter to expectations, embryonic survival and hatch success were significantly improved by air exposure in the control treatment, but not in the elevated CO2 treatments. In both the medium and the high CO2 treatment, the intertidal treatments had no significant effect on the embryonic mortality or hatch success. A prior study had documented 8 h air exposure 2 × day−1 to reduce embryonic survival by nearly 20%, noting desiccation and higher temperatures incurring higher metabolic rates as likely cause27. In our study, metabolism (inferred from heart rate) was significantly reduced at maximum air exposure in the medium CO2 treatment, but elevated at moderate air exposure in the high CO2 treatment. This suggests that prolonged air exposure allows embryos to reduce metabolic rates when reared in moderately elevated CO2 levels. In the control CO2 group, air exposure did not affect heart rate. It must be noted, that all heart rate measurements were performed in water and the larvae hatched before the planned air-exposure heart rate measurements could be undertaken. Interestingly, heart rates were not significantly different between CO2 treatments in the fully submerged group, suggesting CO2 on its own did not cause higher metabolic rates. Another study found heart rates increased with CO2 but only in combination with higher temperatures15. A future study should compare heart rate measurements in- and out of water for a better understanding of the effects of air exposure on heart rates.
Length at hatch was significantly reduced with prolonged air exposure, regardless of CO2 treatment. This is similar to the findings in Jones27, albeit their air exposure treatment was associated with higher temperature (12 °C) instead of lower as in our study. Therefore, we can conclude that the reduction in larval length due to air exposure is robust to the effects of air temperature.
The greatest effects of CO2 and air exposure was on larval survival. Cumulative larval survival from hatch to yolk-sac depletion increased by 70–80% with prolonged air exposure, with the effects increasing with aquatic CO2 levels. This indicates strong carry-over effects from air exposure during the embryonic phase to larval development. Previous work has shown gene expression of key metabolic genes linked to lipid metabolism to be altered in larvae reared in similarly high CO2 waters, likely due to insufficient oxygen supply to the tissues with consequences for late larval development36. We hypothesize that air exposure may have enhanced conditions for oxygen uptake by embryos, lowering metabolic costs of development and improving condition and survival. The mechanisms for the carry-over effect in the present study are yet unknown but will be an interesting topic for further investigation.
We computed the weight to length relationship as a measure of condition for our herring larvae. The slope of this relationship showed that moderate, but not prolonged air exposure in the low intertidal treatment, resulted in an increased weight for a given length, implying improved condition in the control CO2 treatment. On the other hand, prolonged air exposure significantly improved the weight to length relationship, implying improved condition in the elevated CO2 treatments, but not to the levels of the control treatment. A summary of finding are presented in Fig. 1.
Air exposure and temperature
In our study, we are unable to disentangle air exposure and temperature variation and as such can only speculate on the modulating role that the colder temperature might have played. Interestingly, developmental rate and timing of hatch were equal across all tidal and CO2 treatments, even though the tidal treatments experienced much lower temperature during air exposure. Jones27, found herring larvae to hatch a whole day earlier when exposed to 2 × 2 h air exposure at 4 °C warmer temperatures than water. In our study, larval ATUs differed by up to 14 between the subtidal (no air exposure) and high intertidal (5 + 9 h air exposure) treatments with an experienced temperature difference of up to 8 °C, without incurring differences in time to hatch. It therefore seems that the physiology of embryonic herring is rather plastic below temperatures of 10 °C, whereas temperatures above 10 °C can significantly reduce survival, likely due to increased metabolic activity and limited O2 supply37,38. While air exposure may be able to counteract O2 limitation and reduce metabolic costs, exposure to air above 11 °C has been found to increase mortality by 75%31. A future study is needed to understand the role of temperature during air exposure and aquatic acidification.
Effect of substrate
In our study, herring eggs were collected from the wild attached to algae. These algae, in combination with cold, humid condition outdoors, prevented the eggs from desiccating when exposed to air. These algae, however, may have also had some interaction with the embryos. While care was taken to place a random mix of algal substrates (Sargassum, Phyllospadix, Plocamium, Mastocarpus, Callophyllis, Rhodymenia, Chondracanthus) with attached herring eggs into each treatment, both the subtidal and high intertidal in the medium CO2 treatment ended up with the two pieces of Turkish towel (Chondracanthus exasperatus), which saw poor hatch success with high mortalities shortly before hatch. This may have been due to the densely packed eggs on these flat-bladed algae, as opposed to more dispersed egg deposition on the other types of algae, although densely packed eggs are normal for herring spawn. Other than the apparent effect the Turkish towel had on hatch success, there were no observed effects of algal substrate on the herring. A study quantifying the effects of different substrates on Pacific herring found no effect on embryonic survival and hatch success6. Algae, being an autotrophic organism producing O2 from CO2 may have also had some effect on the carbonate system in the micro-environment surrounding the embryos. While the system was set on partial flow-through and water was circulated around the tank, we do not know how the algae might have affected CO2 levels directly in contact with the embryos. Therefore, it is possible that the lack of CO2 effect seen in our study arose from photosynthetic activity, or other substrate chemicals on embryos. Given that this is the natural state in the ocean, this scenario is more relevant than herring embryos reared on artificial substrate. As soon as the herring hatched, algae were removed, thus larval development was not directly affected by algae.
In conclusion
Contrary to our hypothesis, CO2 did not increase mortality and malformations in embryonic and larval Pacific herring in our study. Furthermore, air exposure did not increase mortality or decrease developmental and metabolic rate. While we did not see strong negative effects of elevated CO2 levels on herring development and cranial deformities described by prior studies, air exposure during embryonic development had striking positive effects on larval growth, condition and survival in Pacific herring, with some interactive effects with CO2. This study underlines the importance of considering local conditions when conducting climate change experiments. Only when we place our organism of study into the context of its environment, can we begin to understand how the future climate may impact it, on an individual, population and ecosystem level.
Data availability
The datasets generated during and/or analysed during the current study are available from the corresponding author upon request.
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Acknowledgements
We would like to thank Brenna Collicutt and her crew for support in the wet lab and Alana Cross for providing imaging support. We would like to acknowledge that the experiment was carried out in the traditional territories of the Kwakwaka’wakw and the We Wai Kai Nation.
Funding
This work was funded by the Tula Foundation and the Mitacs Grant to Brian Hunt F19-00303 and a Natural Sciences and Engineering Research Council (NSERC) Discovery grant to Colin Brauner (2018-04172).
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A.Y.F., S.L.R.L., C.J.B. and B.P.V.H. designed the experiment, A.Y.F. and S.L.R.L. conducted the experiment, A.Y.F., S.L.R.L., C.J.B. and B.P.V.H. analyzed the data and wrote the manuscript.
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Frommel, A.Y., Lye, S.L.R., Brauner, C.J. et al. Air exposure moderates ocean acidification effects during embryonic development of intertidally spawning fish. Sci Rep 12, 12270 (2022). https://doi.org/10.1038/s41598-022-16399-6
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DOI: https://doi.org/10.1038/s41598-022-16399-6
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