Fatty acid oxidation fuels glioblastoma radioresistance with CD47-mediated immune evasion

Jiang, Nian; Xie, Bowen; Xiao, Wenwu; Fan, Ming; Xu, Shanxiu; Duan, Yixin; Hamsafar, Yamah; Evans, Angela C.; Huang, Jie; Zhou, Weibing; Lin, Xuelei; Ye, Ningrong; Wanggou, Siyi; Chen, Wen; Jing, Di; Fragoso, Ruben C.; Dugger, Brittany N.; Wilson, Paul F.; Coleman, Matthew A.; Xia, Shuli; Li, Xuejun; Sun, Lun-Quan; Monjazeb, Arta M.; Wang, Aijun; Murphy, William J.; Kung, Hsing-Jien; Lam, Kit S.; Chen, Hong-Wu; Li, Jian Jian

doi:10.1038/s41467-022-29137-3

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Published: 21 March 2022

Fatty acid oxidation fuels glioblastoma radioresistance with CD47-mediated immune evasion

Nian Jiang^1,2,
Bowen Xie^1,3,
Wenwu Xiao⁴,
Ming Fan¹,
Shanxiu Xu⁵,
Yixin Duan¹,
Yamah Hamsafar⁶,
Angela C. Evans¹,
Jie Huang¹,
Weibing Zhou^1,7,
Xuelei Lin²,
Ningrong Ye²,
Siyi Wanggou²,
Wen Chen^1,7,
Di Jing^4,7,
Ruben C. Fragoso^1,8,
Brittany N. Dugger⁶,
Paul F. Wilson^1,8,
Matthew A. Coleman^1,8,
Shuli Xia⁹,
Xuejun Li^2,10,
Lun-Quan Sun ORCID: orcid.org/0000-0002-0558-5250¹¹,
Arta M. Monjazeb^1,8,
Aijun Wang ORCID: orcid.org/0000-0002-2985-3627⁵,
William J. Murphy ORCID: orcid.org/0000-0002-2793-401X^8,12,
Hsing-Jien Kung^4,13,
Kit S. Lam ORCID: orcid.org/0000-0002-3076-6969^4,8,
Hong-Wu Chen ORCID: orcid.org/0000-0003-0177-6989^4,8,14 &
…
Jian Jian Li ORCID: orcid.org/0000-0003-3694-9675^1,8

Nature Communications volume 13, Article number: 1511 (2022) Cite this article

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Abstract

Glioblastoma multiforme (GBM) remains the top challenge to radiotherapy with only 25% one-year survival after diagnosis. Here, we reveal that co-enhancement of mitochondrial fatty acid oxidation (FAO) enzymes (CPT1A, CPT2 and ACAD9) and immune checkpoint CD47 is dominant in recurrent GBM patients with poor prognosis. A glycolysis-to-FAO metabolic rewiring is associated with CD47 anti-phagocytosis in radioresistant GBM cells and regrown GBM after radiation in syngeneic mice. Inhibition of FAO by CPT1 inhibitor etomoxir or CRISPR-generated CPT1A^−/−, CPT2^−/−, ACAD9^−/− cells demonstrate that FAO-derived acetyl-CoA upregulates CD47 transcription via NF-κB/RelA acetylation. Blocking FAO impairs tumor growth and reduces CD47 anti-phagocytosis. Etomoxir combined with anti-CD47 antibody synergizes radiation control of regrown tumors with boosted macrophage phagocytosis. These results demonstrate that enhanced fat acid metabolism promotes aggressive growth of GBM with CD47-mediated immune evasion. The FAO-CD47 axis may be targeted to improve GBM control by eliminating the radioresistant phagocytosis-proofing tumor cells in GBM radioimmunotherapy.

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Introduction

Glioblastoma multiforme (GBM) is the most aggressive brain cancer in adults with the worst prognosis. Radiotherapy (RT) and/or chemotherapy with surgery are the major therapeutic modalities in control of GBM growth. However, the overall survival of GBM patients remains very low as 14.6 months with only about 5.6% 5-year survival rate^1,2,3. Encouragingly, clinical benefits of combined radiation/immunotherapy (radioimmunotherapy) are evidenced in an array of solid tumors including GBM^4,5,6. Promisingly, the tumor immunogenicity potentially enhanced by RT (the Abscopal Effect) and/or radiation-induced molecules with latent immunomodulatory functions are increasingly identified in radiation-treated tumors^7,8, both of which are believed to contribute to the synergetic cancer control by radioimmunotherapy. However, in addition to such radiation-associated tumor response to immunotherapy, tumor microenvironment may also acquire resistance to immunotherapy under radiation⁹, compromising the effectiveness of immune cell facilitated attacks on tumor cells^10,11. Thus, for further validating and improving the efficacy of the combined modality, in addition to continuing on the elucidation of the radiation-induced immune attackable molecules, potential radiation-associate immune-cold status, especially in the recurrent and resistant tumors, is to be identified.

Metabolic reprogramming is one of the fundamental hallmarks in carcinogenesis and tumor progression featured by increased glycolysis in solid tumors including GBM¹². However, flexible metabolic dynamics is demonstrated in mammalian cells under genotoxic stresses^13,14. The oxidative respiration in mitochondria can be instantaneously adjusted in mammalian cells to meet the energy consumption for fueling cell cycle progression and DNA repair^15,16. Recently we found that burning the saturated fat (i.e., palmitates in diet) by mitochondrial fatty acid oxidation (FAO) improves mitochondrial homeostasis¹⁷. Accumulating new evidence also indicates that in addition to the rudimentary glycolytic pathway, tumor cells are capable of reactivating oxidative phosphorylation (OXPHOS) to meet the increasing cellular fuel demand for repairing and surviving the genotoxic anticancer treatments^18,19. Such bioenergetic flexibility appears to fit well into the increased capacity of tumors for metastasis^20,21,22, in which the fatty acid (FA) is an alternative critical energy resource to meet the high-fuel consumption in aggressively growing cancer cells^23,24,25. FAO is shown to play a critical step for mitochondrial lipid digestion that enhances GBM metabolism^26,27, and targeting purine metabolism or NADPH biosynthesis increases the efficacy of GBM control^28,29. Recently, two groups further reveal that FA metabolism accelerates the incidence of breast cancer brain metastasis³⁰ and that glycolysis is a less-essential uptake for GBM metabolism³¹. However, it is unknown whether the enhanced FA metabolism in tumor cells including the recurrent and radioresistant GBM can generate or boost an immunosuppressive status leading to the aggressive behavior with immune evasion.

CD47 is a well-defined immune checkpoint receptor protecting cells from the phagocytotic elimination by immune cells including macrophages via engagement of SIRPα on cell surface^32,33. Therapeutic benefits by targeting CD47 are demonstrated in a series of pre-clinical and clinical studies in an array of human cancers^34,35,36,37 including GBM treated with radiation and anti-CD47 antibody³⁸, and CD47 is overexpressed in radioresistant breast cancer cells³⁷. Using radioresistant GBM cells, regrown syngeneic mouse GBM, and recurrent tumors of GBM patients, this study reveals an immune evasion function of radioresistant GBM cells through FAO metabolism boosted CD47 anti-phagocytosis. CD47 transcription is activated via FAO-derived acetyl-CoA that acetylates RelA K310 to upregulate CD47 transcription. Blocking FAO not only inhibits aggressive growth of radioresistant GBM cells but diminishes the orthotopically regrown mouse tumors by RT combined with anti-CD47 antibody. Blocking the acetyl-CoA-CD47 pathway is a potential therapeutic approach to sensitize radioresistant tumors to CD47 immunotherapy.

Results

Co-enhancement of FAO and CD47 in recurrent GBM with poor prognosis

Glycolysis is documented to be the major metabolic pathway in cancer cells, increasing evidence suggests that metabolic rewiring with enhanced mitochondrial respiration including FAO is involved in tumor adaptive resistance and metastasis^{39,40,41,42,43}. In this study, we aim to detect that FAO driving cell growth is conjugated with immunosuppressive function via FAO metabolite regulated CD47 expression. We first studied the history of 46 high-grade gliomas (HGG) with primary tumor paired recurrent disease following treatment of radio- and/or chemotherapy after initial surgery (Supplementary Table 1). IHC analysis revealed that the three essential FAO enzymes CPT1A, CPT2, and ACAD9 were increased 86.96, 84.78, and 76.09% respectively in the recurrent gliomas, companied with 82.6% CD47 enhancement (Fig. 1a). The expression of CD47 was positively correlated with CPT1A, CPT2, and ACAD9, respectively (Fig. 1b–d). In agreement, GBM database analysis (http://www.cgga.org.cn/index.jsp) of 504 HGG containing 220 recurrent and 284 primary GBM (not matched) also revealed the elevated expression of CPT1A, CPT2, ACAD9, and CD47 in the recurrent tumors (Fig. 1e). Furthermore, hierarchical clustering of CGGA RNA-seq heat map data revealed that the majority of FAO-related genes were positively correlated with CD47 expression (Fig. 1f). Enrichment analysis further revealed that 157 FAO-related genes were enriched in tumors expressing a high level of CD47 (NES = 1.338, P = 0.002, Fig. 1g). Additionally, Pearson correlation analysis confirmed the positive correlations of CPT1A, CPT2, ACAD9, and CD47, respectively (Fig. 1h–j). Elevated expression of CD47, CPT1A, and CPT2 (except ACAD9) were negatively contributed to the overall survival (OS) (Fig. 1k–n). In agreement with the results above, the association of CD47 with CPT1A or CPT2 were shown as a significant clinical and pathological feature in the HGG patients (Supplementary Table 2). Intriguingly, although both CD47 and PD-L1 play key roles in immune checkpoint regulation, PD-L1, CPT1A isoforms (CPT1B and CPT1C) and ACAD9 isoforms (ACADVL and ACADS) did not show a detectable difference in expression between the primary and recurrent GBM tumors (Supplementary Fig. 1a), whereas CPT1B, ACADL, and ACADM (except CPT1C and ACADVL) were associated with lowered OS (Supplementary Fig. 1b–f). In consistence, Cox regression analysis of HGG patient data further identified a poor survival linked with elevated levels of ACADM, CPT2, CPT1A, CD47, and other factors including 1p19q codeletion, IDH mutation, and age (Supplementary Table 3). In addition, multivariate Cox proportional hazards model suggest that CPT2 (HR = 1.43, P = 0.029), ACADM (HR = 0.707, P = 0.049), 1p19q codeletion (HR = 0.38, P < 0.0001), and IDH mutation (HR = 0.43, P < 0.0001) were applied as independently predicters when considering age, expression of CD47 and CPT1A (P < 0.05) by univariate Cox regression. Based on these clinical data analysis and the well-established rate-limiting FAO cascade of CPT1, CPT2, and ACAD9 in mitochondrial FA transportation and digestion (Supplementary Fig. 1g)^44,45,46, we hypothesized that CPT1A, CPT2, and ACAD9 are the key FAO enzymes for rewiring metabolism by enhancing FA digestion that not only can meet the enhanced cellular fuel demand for tumor cell proliferation but also upregulate CD47 expression to protect tumor cells from macrophage-mediated immune surveillance, leading to the aggressive growth with immunosuppression in the radioresistant GBM.

**Fig. 1: Co-enhancement of FAO enzymes and CD47 links to the recurrent GBM patients and poor prognosis.**

Mitochondrial FAO activity is dominant in radioresistant GBM cells

Based on the above assumption, we tested that the basal glycolysis-dominant metabolism in the untreated tumor could be rewired to the FAO-centered metabolism in the radioresistant GBM cells. An array of radioresistant (RR) GBM cells were thus generated from human (U251, U87, and A172) and mouse (GL261) GBM cells following the established in vitro clinic-mimic radiation schedule (Supplementary Fig. 2a). The RR GBM cells were enriched with glioblastoma stem cells (GSCs) confirmed by specific marks of CD133, OCT4, SOX2, NANOG, and HER2 by Western blot (Supplementary Fig. 2b) with elevated CD133/HER2 population identified by flow cytometry analysis (Supplementary Fig. 2c, d). The enhanced aggressive behavior was measured by clonogenicity, neurosphere formation, migration, and invasiveness (Supplementary Fig. 2e–h). The FA-enhanced feature was recapitulated by PA-accelerated syngeneic RR GL261 tumor growth compared to that with control solvent treatment (Fig. 2a). Compared to wildtype (WT; untreated parental cancer cells) cells, mouse RR GL261 cells demonstrated an increased ATP generation peaked in the range of 12.5–25 µM for 48 h in FA-enriched medium with palmitate (PA) (Fig. 2b). By detecting isotope tracers ([9,10-³H(N)]-palmitic acid [0.5 µCi (~9.3 pmol)]) and ¹⁴C- palmitoyl-carnitine⁴⁷ tracer CPT2 enzymatic activity, RR GBM cell lines rewired the metabolism by increasing FAO rate (Fig. 2c). The boosted mitochondrial FAO activity was further identified by the elevated enzymatic activity of CPT2 measured by the rate of incorporation of labeled ¹⁴C-palmitoyl-carnitine into a pool of palmitoyl (Supplementary Fig. 3a). Furthermore, FAO production acetyl-CoA was also found to be elevated in RR GBM cells (Fig. 2d), whereas the glycolytic activity was lowered with decreased glucose uptake (Fig. 2e) and lactate generation (Fig. 2f) compared to all of their WT untreated counterparts. In addition, along with the increased mitochondrial numbers (Fig. 2g), RR GBM cells demonstrated an enhanced mitochondrial spare OCR which represents the potential adaptive metabolic capacity in comparison to the WT counterparts (Fig. 2h). The accelerated mitochondrial bioenergetics in the RR cells was also demonstrated by maximizing respiration with the uncoupler FCCP (Fig. 2h and Supplementary Fig. 3b–e). Etomoxir (ET) that inhibits CPT1 that couples with CPT2 for mitochondrial transportation of long-chain FA^48,49, was applied to inhibit FAO in RR GBM cells. Aggreged with lowered glycolysis, a remarkably low level of cytoplasmic lipid was detected in RR GBM cells which could be increased by CPT1 inhibition, whereas lipid accumulation in the WT cells was not affected by CPT1 inhibition (Fig. 2i and Supplementary Fig. 3f). Consistently, a substantial reduction of OCR and ATP generation was generated by ET in the RR but not WT cells (Fig. 2j, k and Supplementary Fig. 3g–j). Together, these metabolic features in RR GBM cells contrasted with WT untreated tumor cells indicate a capacity of rewiring the basal glycolytic pathway to an FA-dominant metabolism in RR GBM cells surviving radiation and sustaining an aggressive growth pattern.

**Fig. 2: Enhanced FAO drives the aggressive growth of radioresistant GBM cells.**

FAO-associated CD47 expression causes anti-phagocytosis in regrown GBM

With the observed co-enhancement of FAO and CD47 in GBM prognosis and FAO-associated CD47 expression and anti-phagocytosis in RR GBM cells, we then assumed that in addition to fueling the energy demand for tumor cell survival and proliferation, the boosted FAO metabolism may offer the RR GBM cells with an immune evasion ability by protecting macrophage-mediated phagocytosis via FAO-mediated CD47 expression. Indeed, CD47 protein level was enhanced by radiation (IR 5 Gy) in WT tumor cells and remarkedly elevated in RR GBM cells (Fig. 3a). In addition, the percentage of CD47-expressing cells was increased 1.48- and 2.48-fold in radiation-treated WT and RR U251 cells and 3.2- and 5.97-fold in radiation-treated WT and RR U87 cells, respectively (Fig. 3b and Supplementary Fig. 4a). Furthermore, increased FAO enzymes and CD47 were detected in three human RR GBM cell lines compared to the corresponding parental WT GBM cells (Fig. 3c) and immunofluorescence revealed that co-expression of CPT1A, CPT2, or ACAD9 with CD47 was observed in RR cells (Supplementary Fig. 4b–d). To access the immune-escaping ability, macrophage-mediated phagocytosis was tested on WT and RR GBM cells which demonstrated a lowered phagocytosis rate in all three RR GBM cells compared to the WT counterparts (Fig. 3d and Supplementary Fig. 4e).

**Fig. 3: Activation of FAO and CD47 with anti-phagocytosis in RR GBM cells and regrown tumors.**

Co-enhancement of FAO enzymes with CD47 was then accessed by in vivo U251 tumor model. When tumors reached about 200 mm³, local treatment with fractionated radiation was delivered (2 Gy/day for 5 days) which led to the regrown tumors collected at day 28 (Fig. 3e). The quantitation of IHC staining and western blots demonstrated a substantial number of proteins of CD47 and CPT1A, CPT2, ACAD9 in regrown tumors compared to the control tumors without radiation (Fig. 3e, f and Supplementary Fig. 4f). Then, using GL261 syngeneic orthotopic GBM model with double-labeled Luc/GFP, the regrown tumor can be detected by magnetic resonance imaging (MRI) (Fig. 3g) by observing a significant shrinkage with undetectable tumor images at Day 17 and regrown tumor image at Day 28 (Fig. 3g). The elevated expression of CD47, CPT1A, CPT2, and ACAD9 in the regrown tumors was verified by IHC staining (Fig. 3h and Supplementary Fig. 4g), and macrophage phagocytosis was analyzed and scored by calculating the cells with GFP interacted or engulfed by macrophages, indicating a significantly lowered macrophage phagocytosis in the regrown tumors (Fig. 3i).

FAO upregulates CD47 transcription via acetyl-CoA mediated RelA acetylation

The fact that citrates and acetyl-CoA in the cytoplasm are major FAO metabolites and CD47 can be regulated by NF-κB^50,51 promoted us to test that FAO-derived citrates/acetyl-CoA functions to bridge the FAO metabolism and the CD47-mediated anti-phagocytosis. In consistence with FAO-mediated NF-κB activation, NF-κB luciferase activity was enhanced by PA (25 µM, 48 h) or mitochondrial FAO activator L-carnitine (gradient concentrations for 48 h) (Supplementary Fig. 5a, b) and CD47 mRNA level can be reduced by ET to 60 and 50% respectively in RR U251 and RR U87 cells (Fig. 4a). The NF-κB luciferase activity was reduced in the confirmed CRISPR KO CPT1A^−/−, CPT2^−/−, and ACAD9^−/− cells (Fig. 4b and Supplementary Fig. 5c) that agreed with the reduced activity of CD47 promoter containing NF-κB motif (Fig. 4c) in the CPT1A^−/−, CPT2^−/−, or ACAD9^−/− cells or the NF-κB motif was deleted (Fig. 4c) with CD47 mRNA level remarkedly suppressed (Fig. 4d). NF-κB in coupling FAO and CD47 anti-phagocytosis was further supported by CGGA database analysis that identified a cluster of NF-κB subunits was tightly correlated with the mRNA levels of CD47 in GBM patients, though the correlation was detected in both primary and recurrent GBM (Supplementary Fig. 6a, b), suggesting that FAO-mediated CD47 transcription is associated with FAO metabolites.

**Fig. 4: FAO regulates CD47 transcription via citrate/acetyl-CoA mediated RelA acetylation.**

The citrates released from the FAO-enhanced TCA cycle is converted by ATP citrate lyase (ACLY) to acetyl-CoA that provides the acetyl group for acetylation in posttranslational modification. The citrate and acetyl levels were remarkedly elevated in RR cells (Figs. 2d, 4e) and reduced by ET treatment or in CPT1A^−/−, CPT2^−/−, and ACAD9^−/− cells (Fig. 4f and Supplementary Fig. 7a). CD47 promoter activity was enhanced by citrate treatment whereas no obvious enhancement was observed with the promoter with deleted NF-κB binding motif (Fig. 4g). In addition, SB204990, a specific inhibitor of ACLY, reversed the citrate-enhanced NF-κB activation and CD47 mRNA level (Fig. 4h, i) and also reversed citrate-enhanced RelA acetylation and CD47 expression (Fig. 4j). However, in WT GBM cells, citrate cannot encourage CD47 promoter activity (Supplementary Fig. 7b) although mildly upregulated CD47 transcription which was also reversed by SB204990 (Supplementary Fig. 7c). Directly inhibition with A-485, an inhibitor for p300 regulated RelA acetylation⁵² suppressed NF-κB luciferase reporter activity (Supplementary Fig. 7d) and CD47 promoter activity as well as gene expression which was recuperated in cells expressing RelA or mutant RelA K310R (Fig. 4k, l, and Supplementary Fig. 7e). Interestingly, KAT-specific acetylation sites in human CD47 proteins were predicted (Supplementary Fig. 8a) and also the acetylation of CD47 protein was enhanced by p300 and blocked by A-485 (Supplementary Fig. 8b). Thus, the FAO-mediated CD47 protein acetylation may help to stabilize protein which was indeed reversed by A-485 treatment (Supplementary Fig. 8c). Taking together, these results demonstrate that FAO-derived cytoplasmic acetyl-CoA is able to upregulate CD47 expression and protein stability via acetyl-CoA mediated acetylation on gene transcription and posttranslational modification.

FAO inhibition suppresses radioresistant GBM cells aggressive growth with enhanced phagocytosis

FAO-mediated CD47 anti-phagocytosis was further evidenced by reduction of macrophage phagocytosis in WT U251 and GL261 cells by boosting FAO activity with l-carnitine, PA, or radiation (Fig. 5a and Supplementary Fig. 9a). In addition, ET treatment on RR GBM cells can dose-dependently enhance macrophage-mediated phagocytosis (Fig. 5b, c and Supplementary Fig. 9b). However, a high dose of ET treatment on macrophages cannot increase phagocytosis (Supplementary Fig. 9c). Similar to ET-induced CD47 downregulation, CPT1A^−/−, CPT2^−/−, and ACAD9^−/− cells showed a strikingly reduced protein level of CD47 and CD47 positive cell populations (Fig. 5d and Supplementary Fig. 10a, b) and enhanced phagocytosis (Fig. 5e and Supplementary Fig. 10c, d) that can be enhanced by blocking ACLY or by inhibition of p300 with A-485 (Fig. 5f and Supplementary Fig. 10e). We assumed that such a pro-phagocytotic function by FAO suppression should also be accompanied by weakened aggressive growth of RR GBM cells due to reduced cellular fuel supply. Indeed, ET treatment or in CPT1A^−/−, CPT2^−/−, and ACAD9^−/− cells, radiation-mediated elimination of the clonogenic tumor cells by 25–50% (Fig. 5g and Supplementary Fig. 11a–c). Similarly, apoptotic cell death was enhanced in CPT1A^−/−, CPT2^−/−, and ACAD9^−/− cells or ET-treated cells (Fig. 5h and Supplementary Fig. 11d). Strikingly, the neurosphere formation and sphere sizes which account for the critical aggressive behavior of GBM^53,54 enhanced in RR U251 cells were remarkedly reduced by ET treatment or in CPT1A^−/−, CPT2^−/−, and ACAD9^−/− cells (Fig. 5i, j and Supplementary Fig. 11e).

**Fig. 5: FAO deficiency boosts macrophage phagocytosis with diminished aggressive growth.**

Suppression of orthotopic regrown tumor by blocking of FAO and CD47

In vitro analysis with RR U251 cells revealed substantial macrophage-mediated phagocytosis following treatment with ET (400 µM, 12 h) comparable to the level of anti-CD47 antibody (10 µg/ml, 2 h). However, dual treatment of ET and anti-CD47 antibody further enhanced the macrophagic attack (Fig. 6a and Supplementary Fig. 12). To evaluate the potential synergetic effects of inhibiting both FAO and CD47, the regrown model of mouse orthotopic GL261 tumors expressing with GFP/Luciferase was generated allowing in vivo monitoring tumor growth after radiation (Fig. 6b). Animals were then grouped and fractionated radiation (3 Gy × 3) was delivered to the tumor region of all groups on Day 10 after inoculation when the tumor was visible. The shrinkages of tumor sizes were detected around Day 17 and observed regrowth by Day 24. Tumor volume in each mouse was monitored with the in vivo bioluminescence imaging (Fig. 6c and Supplementary Fig. 13). In the treatment groups, rat anti-mouse CD47 antibody generated from MIAP301 hybridoma cells (16 mg/kg), ET (30 mg/kg), or combination, was administrated via i.p. every other day for 14 days (total seven treatments) till Day 38 and tumor imaging and animal survival were monitored. We found that although tumor sizes were variable within each group, a noteworthy reduction of the regrown tumor volume was detected in mice treated by dual blockage with ET and CD47 antibody compared to ET or anti-CD47 alone although a significant inhibitory effect on the tumor regrowth was observed by each treatment (Fig. 6c, d). In consistence with the in vivo tumor imaging, the animal survival rate was remarkedly enhanced in the group of dual blockades of FAO and CD47 compared to single treatment with one mouse actually surviving by Day 54 (Fig. 7a). These results encouraged us to detect if the macrophage-mediated phagocytotic attack contributes to a key role in such synergetic inhibition on the regrown tumors. Firstly, the potential capacity of anti-CD47 antibody crossing the blood–brain barrier (BBB) was identified by fluorescence analysis. Using the Fluorescent 14-bit images from Zeiss Axioscan.Z1 slide scanning system, the penetrated rat anti-CD47 antibody was detected by interacting to rabbit anti-rat IgG and rhodamine red conjugated goat anti-rabbit antibody on the surface of GFP-labeled tumor cells (Fig. 7b, c and Supplementary Fig. 14). Indeed, tumors treated by anti-CD47 antibody showed an increased antibody binding to tumor cells, whereas tumor binding antibody was less intensified in tumors treated with ET, indicating again that blocking FAO reduces CD47 expression. With similar reasoning, the tumor binding antibody in tumors with dual inhibition could not be enhanced to the level of anti-CD47 treatment, suggesting an increased tumor cell death synergistically induced by blocking FAO and CD47. Consistent with inhibited tumor regrowth and antibody penetration in the combined treatments, increased macrophage-mediated phagocytosis on tumor cells was evidenced in combined treatment with ET and anti-CD47 antibody (Fig. 8a, b and Supplementary Fig. 15), although radiation with ET or anti-CD47 treatment also inspired a certain level of macrophage infiltration and phagocytosis on the tumor cells.

**Fig. 6: Radiation with blocking FAO and CD47 diminishes tumor regrowth.**

**Fig. 7: Animal survival and anti-CD47 antibody tumor penetration in combined treatment.**

**Fig. 8: Enhanced macrophage phagocytosis of tumor cells in combined treatment.**

Discussion

Fundamental mechanisms driving aggressive growth in the resistant and recurrent malignancy with life-threatening conditions remain as the major challenge in GBM treatment, although many milestones have been covered in improving the efficacy of treatment for other cancers. Using radioresistant cells and orthotopic regrown GBM, the present study uncovers an anti-phagocytotic feature in recurrent GBM due to FAO-regulated CD47 expression. The glioblastoma stem cell (GSC)-enriched RR GBM cells are able to rewire the basal glycolysis-dominant metabolism to FAO-centered pathway which functions not only to fuel the cellular energy consumption demand for aggressive tumor growth but also, coordinatively, to defend the resistant cancer cells from macrophage-mediated phagocytosis. Such an alliance of FA-driven aggressive proliferation protected by CD47 anti-phagocytosis illustrates a unique survival advantage in the recurrent GBM cells. Thus, blocking the FAO-CD47 axis may eliminate the resistant cancer cells equipped with a boosted cellular energy fuel supply and CD47-mediated protection against macrophagic attack.

The glycolysis-dominant metabolism is a well-defined hallmark in malignant cells and glycolysis is shown to inactivate the immune cell activity causing an immune “cold” status^55,56. However, it has been unclear if such a glycolysis-dominant pathway could be rewired or replaced by a high energy output metabolism in tumor cells that are capable of surviving genotoxic anticancer modalities including radiation. Indeed, a dynamic feature of tumor metabolism is recently identified in GBM radiosensitivity²⁹. Such a programmatic metabolic ability is also detected in the present study using radioresistant GBM cells and a regrown mouse GBM model with enhanced lipid digestion for fueling the aggressive behavior of radioresistant GBM cells. The rewired metabolism is evidenced by reduced lactate generation with elevated FA metabolic capacity, accelerated cytoplasmic lipid turnover rates, increased mitochondrial OCR and ATP output. The FA-overriding is also illustrated by the results that inhibition of FAO remarkedly affected the radioresistant but not the parental untreated wild-type GBM cells, indicating that the rewired FA metabolism contributes to radiation-associated acquired tumor resistance. Shifting from glycolytic ascendency, the RR GBM cells show an increased spared respiratory capacity (SRC) that is related to the adaptive mitochondrial bioenergetics in cancer cell survival in radio- or chemotherapy^57,58. In the light of FAO being able to promote SRC⁵⁹, we found that the elevated SRC is indeed involved in RR GBM cells following the glycolysis-FAO rewiring. Together, these findings support an adaptive FA-dominant metabolism in radioresistant GBM cells offering the survival advantage and aggressive behavior.

The FAO-mediated CD47 expression may contribute to the overall tumor adaptive (acquired) resistance with the feature of aggressive growth and anti-phagocytosis. A recent study demonstrates that CD47 knockout aggravates the lipid accumulation in normal liver cells⁶⁰ supporting tight coordination between FAO and CD47 expression. Among the potential key elements responsible for FAO-regulated CD47 expression, FAO-enhanced citrate level is shown to activate the CD47 promoter for gene transcription, thus functioning as the bridging factor between reprogrammed FA metabolism and CD47-mediated immune-escaping ability (Fig. 8c). The argument could be that the acetyl-CoA can also be generated by glycolysis in tumor cells. However, with the significantly lowered glycolysis in the RR GBM cells, it is likely that most cytoplasmic acetyl-CoA is resulted from FAO-derived citrates to form acetyl-CoA catalyzed by ACLY. In addition, acetyl-CoA is well defined to regulate fundamental cellular functions via protein lysine acetylation^61,62,63 including the prosurvival lipogenesis and DNA synthesis^64,65,66. Indeed, NF-κB RelA K310 acetylation and CD47 promoter activity enhanced by acetyl-CoA were blocked by inhibition of ACYL with SB204990. Interestingly, predicted KAT-specific acetylation sites were also identified in the CD47 protein sequence and indeed, acetyl-CoA mediated CD47 acetylation demonstrated a reduced protein degradation (Supplementary Fig. 8). Therefore, the overall status of FAO-generated metabolic rewiring can activate multiple layers of signaling pathways for prosurvival and immune escaping in the GBM cells surviving radiotherapy. Further definition of the FAO metabolites that regulate other immune checkpoint and/or immune regulating factors will be a significant advance in revealing the dynamic immunosuppressive status in tumor microenvironment under radiotherapy. This study also suggests that tumors resided in a lipid-enriched microenvironment such as GBM and breast cancer brain metastasis, CD47-mediated immunosuppressive status could be readily adopted by the tumor cells via rewiring FA combustion with aggressive growth and immune evasion. However, in addition to citrate converted acetyl-CoA, other FAO-derived metabolic intermediates could also be involved in the checkpoint gene regulation including NADPH and reactive oxygen species (ROS), both have been identified in FAO-dominant cells and required for de-novo lipogenesis in promoting cancer growth^67,68.

The lipids released from the dead tumor cells in a tumor microenvironment treated by radiation and immunotherapy can also offer the survival advantage for cancer stem-like cells (CSCs) which are enriched in radioresistant tumor^{57,59,69,70,71} via FAO-mediated self-renewal and metastasis^22,70. Our present work further reveals that FAO-CD47-mediated anti-phagocytosis coordinatively contributes to the aggressive behavior of resistant tumor cells enriched with GSCs in the regrown tumors with the heterogeneity that is well-defined⁷². Thus, a metabolic reprogramming-associated immune-cold status in the GSC-enriched radioresistant/recurrent GBM holds a latent value in predicting tumor response to immunotherapy and in inventing new metabolic targets in the combined immunotherapy with radiation or chemotherapy. Furthermore, referred to as an Achilles’ Heel of GSCs, the feature of DNA replication stress in radiation DNA damaging response^73,74 may also be tightly connected to the FAO dynamics since FAO serves the major cellular fuel for GSCs surviving radiation. Further deciphering the coordinative mechanism between a dynamic fatty acid metabolic rewiring and the anticancer immune enforcement in CSCs and GSCs will be of importance for discovering more effective CSC/GSC-targeting agents.

Ideally, an immune hot status favors the anti-tumor immunotherapy since immune cells can be activated by the cytotoxicity induced by anticancer therapies in the tumor microenvironment^75,76. Like other critical immune checkpoints such as CTLA-4, PD-L1, and PD-1, CD47 has been extensively studied with anticipated innate and adaptive immune system responses. An adaptive tumor immune resistance to CD47 immunotherapy is reported via suppressing radiation-associated tumor immunogenicity by hijacking caspase 9 signaling pathway⁹. The FAO-inducible CD47 promoter activity further supports a dynamic CD47 level in an irradiated tumor microenvironment. Therefore, such increased intensity of CD47 receptors on an RR GBM cell or a GSC will allow it to effectually escape the immunosurveillance of phagocytotic attack by immune cells. In addition, increased receptors on the RR GBM cells may efficiently consume anti-CD47 antibodies before it reaches the threshold concentration sufficient for tumor cell elimination, especially in the case of GBM with the challenge of antibody crossing the blood–brain barrier (BBB) or blood–tumor barrier (BTB). Our present study illustrates a variable tumor immune evasion ability due to metabolic rewiring status in the surviving cancer cells.

Checkpoint immunotherapy requires an efficient and consistent antibody concentration in the targeted tumor tissue which poses a challenge for GBM immunotherapy due to the limitation of BBB and/or BTB. It is reported that GBM breaks the integrity of BBB, making it possible to deliver a fraction of circulating drugs or IgG into the tumor parenchyma^{77,78,79,80,81}. In addition to macrophages recruited from the periphery, microglia are found to be effector cells of brain tumor cell phagocytosis in response to anti-CD47 blockade³⁵. Using the same mouse GL261 tumor, Gholamin et al.⁸² demonstrate that the anti-CD47 antibody is able to penetrate the BBB, and blood concentration around 200 µg/ml can achieve 20 μg/ml in cerebrospinal fluid in the medulloblastoma bearing mouse. In consistent, the present study reveals the anti-CD47 antibody is indeed able to penetrate BBB and bind to tumor cells in irradiated orthotopic GL261 tumors treated with anti-CD47 antibody or combined with FAO inhibitor. These findings, in the line with radiation-enhanced BTB permeability of checkpoint immunotherapy⁸³, suggest a therapeutic advantage of radiation-mediated BTB permeability in antibody infiltration.

It is worthy of noticing that radiation can enhance GBM response to anti-CD47 antibody³⁸. It is thus highly possible that in addition to damage-associated molecular patterns (DAMPs), the radiation-induced FAO-CD47 axis may at least in part lead to the synergistic efficacy enhancing the CD47-expressing cells for being targeted by the therapeutic antibodies. The effective tumor control by CD47 immunotherapy is being increasingly demonstrated. Untangling the FA-mediated tumor immune evasion function may help to elucidate the mechanistic insights between irradiated tumor cells and the infiltrated immune cells. However, the FAO-boosted CD47 overexpression although beneficial for enhancing tumor response to targeted antibody, may cause adverse effects including raising the threshold of antibody concentration for eliminating tumor cells and exhaustion of the infiltrated immune cells. We thus assume that a cluster of immunosuppressive genes including CD47 is involved in the adaptive immunotolerance responsive to genotoxic anti-tumor modalities, which is related to the immune evasion and metastasis due to adaptive response in treated tumors^84,85. Radiation-induced metabolic rewiring may also be affected by radiation quality including the tumor-delivered dose and time⁸⁶. Compared to high doses of radiation, lower doses of radiation are shown to induce high tumor immunogenicity and tumor response⁸⁷ or to increase the molecules of DAMPs required for enhancing immune attack to tumor cells⁸⁸. Therefore, elucidating the multiple communications between treated tumor cells and host immune surveillance will provide valuable information for inventing effective targets in the combined modality of radiation with immunotherapy.

In conclusion, this study reveals that glycolysis to FAO rewiring in radioresistant GBM cells can boost aggressive growth with CD47-mediated immune evasion via FAO-enhanced acetyl-CoA. Such FAO-driven intrinsic growth potential synchronized with the evasion to extrinsic immune cell attacks is identified in radioresistant GBM cells and recaptured in mouse regrown tumors and clinically treated tumors. This harmonic prosurvival coordination between lipid metabolic dynamics and the anti-phagocytotic function represents a highly flexible adaptive capacity of tumor cells under genotoxic conditions, also highlighting a vital role of metabolic rewiring in the immune-escaping ability of tumor cells. The FAO-CD47 axis is a potential target to eliminate the radioresistant and anti-phagocytotic tumor cells in GBM radioimmunotherapy.

Methods

Cell lines and other reagents

Human monocytic THP1 cells and GBM U87 cells were purchased from American Type Culture Collection. U251 and GL261 cell lines were provided By Dr. Kit Lam’s lab at the University of California Davis. A172 cells were kindly provided by Dr. Rajesh Khanna at the College of Medicine, University of Arizona. Three human cell lines U251, U87, and A172 were confirmed by Short Tandem Repeat (STR) analysis. The MIAP301 hybridomas were obtained from Dr. William Frazier (Washington University School of Medicine). Both human and mouse radioresistant GBM cell lines were generated by in vitro radiation using a Cabinet X-ray System Faxitron Series (dose rate: 0.028 Gy/min; Hewlett Packard, McMinnville, OR, USA) with fractionated doses (2 Gy × 20; total accumulated dose = 40 Gy). The surviving clones were selected based on western blot detection using CD133 antibody and maintained in the same medium formula as the parental cells. Experiments were conducted within ten passages after the termination of FIR treatment. U251 cells were cultured in MEM medium (CORNING Cellgro, Catalog #10-010-5 CV) supplemented with 10% FBS (CORNING, Catalog #35-010-CV), 0.1 mM NEAA (CORNING Cellgro, Catalog #25-025-CI), 1 mM sodium pyruvate (CORNING Cellgro, Catalog #25-000-CI), 10 mM Hepes (VWR, Catalog #97064-360) and 100 U/ml penicillin (Roche), and 100 μg/ml streptomycin (Roche). U87, A172, and GL261 cells were maintained in DMEM medium (CORNING Cellgro, Catalog #10-013-CV) containing 10% FBS, 100 U/ml penicillin, and 100 μg/ml streptomycin. THP1 cells were cultured in ATCC-formulated RPMI-1640 medium supplemented with 10% FBS, 15 mM Hepes, 4.5 g/L glucose, 2-mercaptoethanol to a final concentration of 0.05 mM, 100 U/ml penicillin, and 100 μg/ml streptomycin. Cells were cultured at 37 °C and 5% CO₂. The MIAP301 hybridomas were cultured in IMDM medium (GLBCO, Catalog #1240-053) supplemented with 20% FBS and 100 U/ml penicillin and 100 μg/ml streptomycin. Oil Red O (Catalog #O-0625), palmitic acid (Catalog #P5585)), A-485 (Catalog #SML2192), and etomoxir (Catalog #E1905) was obtained from Sigma-Aldrich (St. Louis, MO, USA). SB204990 was purchased from AdipoGen (Catalog #AG-CR1-3697). The anti-human CD47 antibody Hu5F9-G4 was kindly gifted from Dr. Irving Weissman.

Human GBM studies

Pathological slides of 46 GBM patients with primary biopsy matched with recurrent tumors with a history of radio- and/or chemotherapy after surgery were included in this study (Supplementary Tables 1–3). The patient’s information on diagnosis and treatment was obtained with the approval of the Human Ethics Committee of Xiangya Hospital, Central South University, and informed consent from all patients. All of the 46 patients underwent intracranial surgery during a period from March 2010 to January 2019, and 34 of them received radiotherapy after the first surgery. All pathological slides of primary matched with recurrent tumors were evaluated according to H&E staining.

Animals

Animal use and care protocol of in vivo radiation treatment were approved by the Institutional Animal Use and Care Committee of the University of California Davis (IACUC 15315). Animals were housed and cared for according to standard guidelines with free access to food and water. All experiments were performed on 6 weeks old female C57BL/6 mice (Taconic) or 6 weeks old female nude mice (The Jackson Laboratory, Stock No:002019). Animals including littermates of the same sex were randomly assigned to control or treatment conditions.

Immunohistochemistry (IHC)

Tumor tissues were fixed with 10% formalin followed by an embedding in paraffin wax and sectioned at 5 μm thickness. After deparaffinization and rehydration, antigen retrieval was conducted with citrate buffer (pH 6.1) at 95 °C for 40 min and endogenous peroxidase activity was blocked with 10% horse serum before incubation with primary and secondary antibody using Vectastain ABC Kit (Catalog #PK-6100, Vector Laboratories). The slides were then reacted with a DAB peroxidase substrate kit (Catalog #SK4100, Vector Laboratories) and counterstained with hematoxylin (Catalog #H-3401-500, Vector Laboratories). The target protein expression levels were estimated by grading the staining intensity and calculating the positive cells as the following: 0: negative staining; 1: weak; weak staining intensity in more than 25% of tumor cells; 2: moderate; moderate staining intensity in more than 25% of tumor cells; 3: strong; strong staining intensity in more than 25% of tumor cells. For multispectral imaging and analysis, the InForm 2.4 software (PerkinElmer) was used to batch analysis of multispectral images from the experiment. To build an algorithm for tissues and cells segmentation, a few representative multispectral images from the experiment were loaded into InForm software. Areas with non-tumor tissues were excluded before cells segmentation, the segmented tissues and cells were trimmed according to Hematoxylin or DAB signals. For scoring, three to six representative regions of interest from each case were chosen by the pathologist for high-powered (200×) imaging. After the detected tissues compartments were selected and quantified for each stained protein in slides, thresholds for “positive” staining and the accuracy of phenotypic algorithms were optimized and confirmed by the pathologist. 0–3 + (4-bin) scoring system was used to quantify proteins levels. The objective images were then loaded into InForm and the established algorithm was used for IHC scoring. The score system can be used to calculate H-score with cell stains. It included four levels (0/1+, 1+/2+, 2+/3+, 3+), score results were shown by the percentage positivity of cells with each bin. H-score was calculated using the percentages in each bin and ranges from 0 to 300. Batch process all remaining multispectral images from the experiment.

CGGA cohort

CD47 expression was correlated with expression of FAO enzymes CPT1A CPT2, and ACAD9 in primary versus recurrent tumors from the CGGA database (504 HGG containing 220 recurrent and 284 primary GBM; the recurrent tumors were not individually matched with primary tumors). Additionally, gene set enrichment analysis was conducted with a cluster of 157 FAO-related genes in CD47 high or low expressing tumors to generate a coordinative enrichment score and normalized enrichment score (NES) generated with GSEA software. Kaplan–Meier overall survival (OS) analysis was derived from the CGGA database categorized by high (n = 252) or low (n = 252) expression of FAO enzymes and CD47 expression.

Bioinformatics analysis

The information on Glioma samples was obtained from the Chinese Glioma Genome Atlas organization (http://www.cgga.org.cn). The RNA-seq data were normalized for analyzing relative expression, and the overall survival rate of GBM patients was evaluated using the Kaplan–Meier method. The statistical differences in survival length were determined using the log-rank test. Heatmaps, clustering analyses, and correlation analyses were performed using the ggplots, fgesa, and limma R language packages, respectively.

ATP generation

Cellular ATP generation was measured by luciferase ATP assay. Cells were seeded in 96-well plates and cultured for different time intervals according to the experiments. Cells were washed with cold phosphate-buffered saline (PBS) twice followed by extracting cellular ATP using 80 μl of cold 0.5% (w/v) trichloroacetic acid (TCA) and shaking on ice for 20 min. The extraction was then neutralized by adding140 μl of 250 mM Tris-acetate buffer (pH 7.75) and 10 μl of neutralized ATP extracts was mixed with 40 μl of fresh luciferin-luciferase reagent (20× reaction buffer, 0.1 M DTT, 10 mM Luciferin, 0.25 μl luciferase; Invitrogen), and the ATP concentrations were detected by Turner Biosystems 20/20 Luminometer (Promega) and the results were normalized to the protein concentration using the BCA method (Pierce, Rockford, IL).

Radioactive FAO rate measurement

The cellular FAO was determined following the published literature⁸⁹ Briefly, 3 × 10⁵ cells were seeded into each well of a 12-well plate. After 12 h incubation, [9,10-³H(N)]-palmitic acid [0.5 µCi (~9.3 pmol)] (PerkinElmer, Catalog# NET043001MC) in 15 µl carrier solution (1 mM sodium palmitate, 0.17 mM BSA, 150 mM NaCl) was added to each well and incubated for 6 h. The [9,10-³H(N)]-palmitic acid was converted to ³H₂O via mitochondria FAO. To control the background, each experiment contained cell-free control. After labeling, 0.5 ml out of 1 ml culture medium was collected into a 15 ml tube with 100 µl of 1.2 N HCl, and an uncapped 0.5 ml tube containing 0.25 ml of sterile distilled water was carefully inserted into the tube and further incubated for 3 days, and the radioactivity present in the water and medium were determined by scintillation counting. The FAO rate of each sample was calculated and normalized to protein concentration.

Acetyl-CoA assay

Commercially available fluorometry-based assay (MAK039, Sigma-Aldrich, Ontario, Canada) was used. Cells were collected from 100 mm dish plate after reaching 80% confluency and lysed with lysis buffer (20 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1%Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na₃VO₄, 1 µg/ml leupeptin, and 1 mM PMSF). Cell lysates were deproteinized by 1 M perchloric acid and neutralized by 3 M potassium bicarbonate solution to make the final pH in the range of 6–8. About 50 μl of the diluted sample (~50 µg total protein) was mixed with an equal amount of the reaction mix (41.8 µl Acetyl-CoA Assay buffer, 2 µl Acetyl-CoA substrate mix, 1 µl conversion enzyme, 5 µl Acetyl-CoA enzyme mix, 0.2 µl fluorescent probe). Each experiment contained a blank control to omit the conversion enzyme in the reaction mix. Samples were prepared in triplicate in a 96-well plate at 37 °C for 10 min in dark. Fluorescence intensity was measured by using the fluorescence Microplate spectrophotometer (Molecular Devices) at λ_ex = 535/λ_em = 587 nm. The results were then normalized to input protein content.

Glucose detection

The levels of glucose in the cell culture medium of four paired wildtype and RR GBM U251, U87, A172, and GL261 cell lines were measured by glucose assay kit (Catalog #A-114, Biomedical Research Service Center, Buffalo, NY, USA). According to the manufacturer’s protocol, 2 × 10⁴ cells were seeded per well in 96-well plates, incubated for 24 h at 37 °C with 5% CO₂. About 25 μl cell culture medium was deproteinized by precipitation with an equal amount of PEG Solution, vortex and incubated on ice for 30 min and spin down at 13, 15,800×g for 5 min at 4 °C. Supernatants were harvested and diluted 20- to 40-fold with distilled H₂O. The reaction was initiated by adding 50 μl of Glucose Assay Solution and incubated at 37 °C for 30 min. Absorbance was measured using the fluorescence microplate spectrophotometer (Molecular Devices) at 492 nm. Glucose concentrations were calculated by derived equation from standards performed at the same time times dilution factor and normalized based on cell number.

l-Lactate assay

To determine the energy shift in RR GBM cells from the status of glycolysis to oxidative phosphorylation, l-Lactate concentration in four paired wildtype and RR GBM U251, U87, A172, and GL261 cell lines were tested using the l-Lactate Assay kit (Catalog #A-108L, Biomedical Research Service Center, Buffalo, NY, USA). According to the manufacturer’s protocol, 3 × 10⁴ cells were seeded per well in 96-well plates, incubated for 24 h at 37 °C with 5% CO₂. About 50 μl cell culture medium was mixed with an equal amount of PEG Solution, vortexed and incubated on ice for 30 min followed by centrifuging at 15,800×g for 5 min at 4 °C, and diluted 20 μl supernatant by fivefold ice-cold dH₂O (80 μl) prior to assay. The reaction was initiated by adding 50 μl of lactate assay solution and stopped by the addition of 50 μl of 3% acetic acid. The l-Lactate level was measured using the fluorescence microplate spectrophotometer (Molecular Devices) at 492 nm. Plot lactate standards vs. samples respective OD (492 nm). Due to the ten times dilution of samples by PEG and dH₂O, the result needs to be multiplied by the dilution factor.

Mitochondria mass

pERFP-N1-MTS plasmids were constructed in frame insertion of an 87 base MTS (mitochondria targeting sequence) fragment⁹⁰. Cells were cultured in pre-coated 15 mm round cover Glass with 0.01% poly-l-lysine. TurboFact transfection reagent (Thermo Scientific. Catalog #R0534) was used according to manufacture instructions. After 48 h transfection, cells were washed two times with 1X PBS and followed by fixation in 40% paraformaldehyde about 20 min and two times washes with 1X PBS. High-resolution fluorescence images were acquired using a 63× oil lens of Zeiss Observer.Z1 microscope. Fluorescence intensity was quantified using Axiovision software (Zeiss, Germany). Mitochondria mass was quantified by Image J software (NIH Image).

OCR

The extracellular oxygen consumption was measured using the Extracellular Oxygen Consumption Assay kit (ab197242, Abcam). Cells were plated in 96-well plates (3 × 10⁴ cells/well) and cultured overnight at 37 °C, then replaced with 90 µl of fresh culture media and added 10 μl of extracellular O₂ consumption reagent into each well. The wells without O₂ consumption reagent were served as blank controls. All the wells were then sealed with 100 μl of prewarmed high-sensitivity mineral oil. The real-time kinetic analysis of OCR was read at 1.5 min intervals for 180 min at 37 °C using the fluorescence microplate reader (Molecular Devices, Sunnyvale, CA, USA) with the ratio EX/EM = 380/650 nm. The OCR was analyzed by calculating the rate of signal for each assay profile.

FAO assay

FA oxidation, the key step in mitochondrial fatty acid metabolism, was detected using the Assay kit (ab217602) in combination with the Extracellular Oxygen Consumption Assay kit. Cells were cultured overnight and were washed with 100 μl of prewarmed FA-free Medium three times followed by adding 90 μl of FA medium containing FAO conjugate (150 μM oleate-BSA). The wells without cells were included as the basal signal controls. The wells with FA medium only were used as blank control. For the FA-Free control wells, 85 μl of FA-free medium was added plus 5 μl of BSA to keep the consistent BSA concentration in samples containing FAO conjugate. To inhibit FAO, CPT1 inhibitor etomoxir was added into the testing wells 0.5 h before detection. To obtain the maximal mitochondrial OCR, a mitochondrial uncoupler carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP; 0.625 μM) was applied immediately prior to the assay and 10 μl of extracellular O₂ consumption reagent was supplemented to all samples. For the blank control wells, 10 µl of FA measurement media was added to replace the extracellular O₂ consumption reagent. The spare OCR was calculated by retraction of basal OCR from maximal OCR. FAO-driven OCR was calculated by the disparities of signal change rates between the ET-treated group and shamed control group.

Western blotting

The total protein was extracted from cells using RIPA lysis buffer, separated in SDS-PAGE, and transferred onto PVDF membranes. After blocking with 5% milk for 1 h, the membranes were probed with primary antibodies shaking at 4 °C overnight, followed by secondary antibody conjugation at room temperature for 1 h. The membranes were visualized using the ECL Western Blotting Detection system (GE Healthcare) or chemiluminescence western blot detection system (BioRad, Hercules, CA, USA). The following primary antibodies were used: anti-HER2 (C-18, SC-284) and anti-CPT2 (G5, SC-377294) antibodies were bought from Santa Cruze Biotechnology (Santa Cruze, CA, USA). anti-CPT1A (D3B3, Catalog# 12252), anti-OCT4 (C30A3, Catalog# 2840), anti-SOX2 (D6D9, Catalog# 3579) anti-NANOG (D73G4, Catalog# 4903), anti-Histone H3(D1H2, Catalog#4499), and anti-acetylated-Lysine (Catalog#9441) antibodies were from Cell Signaling Technology (Beverly, MA, USA). Anti-ACAD9 antibody was kindly gifted from Dr. Vockley Lab. Anti-CD47 (Catalog# PA2223) antibody were from Boster (Pleasanton, CA, USA). Anti-β-actin (Catalog# A2066) was from Sigma-Aldrich, anti-NF-κB p65 (acetyl K310) (Catalog# ab19870), and anti-Histone H3 (acetyl pan, Catalog# ab47916) antibodies were bought from Abcam (Cambridge, MA, USA).

Flow cytometry analysis of CD47 or CD133/HER2 positive cells

Cells that reached 80% confluency were exposed to a single dose of 5 Gy irradiation and were collected after further culture for 15–16 h and the pellets were rinsed with washing buffer (PBS containing 0.5% BSA). Cell pellets were resuspended in pre-diluted FITC mouse anti-human CD47 antibody (BD Pharmingen Catalog #556045) and incubated in room temperature for 1 h in dark, followed by three times washing with washing buffer. For detection of the co-expression of stem cell markers on RR U87 and RR U251, the double staining of PE-CD133 (Miltenyi Biotec, Catalog #130-080-801), and APC-HER2 (R&D Catalog #FAB1129A) were performed. All antibodies were titrated to optimized conditions. The FACS Canto II cytometer (BD) and FlowJo (Three Star, Ashland, OR, USA) were used for analysis. The gating strategies were based on FSC and SSC. The appropriate channels of cell populations, such as unstained and positive control, were gated as standard.

Clonogenic survival

The radiosensitivity of RR GBM cells with or without CRISPR knockout of FAO genes or treated with CPT1A inhibitor ET were estimated using the clonogenicity assay. Cells were constantly passaged every two days until reaching 80% confluency. Cells were exposed to irradiation (5 Gy) and 500 cells were following seeded into six-well plates. Both irradiated and control cells were cultured for 10 to 14 days. Colonies were fixed and stained with Coomassie blue. The colony with more than 50 cells were counted. The colony images were obtained by Nikon microscope (Eclipse, E1000M, Japan). The clonogenicity were calculated by the percentages of colonies formed from seeded cells in each group.

Neurosphere formation

Parental wild-type and RR GBM cells with or without CRISPR knockouts of FAO genes were subjected to neurosphere formation assessment. For the RR GBM cells without FAO knockout, cells were treated with CPT1A inhibitor ET (200 μM, 24 h). The ET-treated cells and FAO KO cells were then irradiated with 5 Gy and non-radiated control and irradiated cells were collected and filtered through a 40-μm cell strainer, and 1000 cells suspension were added into 35 mm Petri dish in triplicates. The DMEM/F12 neurosphere medium is supplemented with 1×B27 (Life Technology, Carlsbad, CA, USA), 20 ng/ml EGF (Biovision, Mountain View, CA, USA), 10 ng/ml FGF (Biovison, Milpitas, CA. USA), 5 μg/ml insulin, 1% FBS. Cells were maintained in sphere formation medium for 7–10 days and fresh medium were supplemented every 2 days. The spheres with size exceeding 40 µm were counted and calculated in triplicates.

Gap filling assay

Gap filling assay was used for revealing cell migration capacity. In total, 1 × 10⁶ cells were seeded into six-well plates and when reached 100% confluence, the medium was replaced with a 1% FBS starvation medium and cells were further cultured for 24 h before the gap was created by scraping the dish diagonally with a sterile tip. The cell migration capacity was monitored at 0, 12, 24, and 48 h post scraping. Images were obtained by phase-contrast microscopy, and the gap filling ability of cells was estimated by measuring the gap distance and quantitation using Image J (NIH Image).

Transwell invasion assay

Matrigel (Cat #356231, BD Biosciences) was diluted with the coating buffer: 0.01 M Tris (pH 8.0), 0.7% NaCl at a final concentration of 200–300 µg/ml (1:40–45 dilution from stock), and 100 μl of the diluted matrigel were added into the upper chamber of 24-well transwell (Costar catalog #3422, Corning, NY) and incubated at 37 °C for 2 h for gelling. The coating buffer was then removed from the permeable support membrane and the cells to be tested were resuspended in a medium containing 1% FBS at a density of 2.5 × 10⁴ cells/300 μl before adding onto the upper chamber. The lower chamber was then filled with 800 μl of fresh cell culture medium containing 5 μg/ml fibronectin (Catalog #SC-29011, Santa Cruz). Cells were incubated for 48 h and the cell-penetrating capacity was measured with the membrane stained with Diff-Quick Stain kit (K7128, IMEB INC).

CPT2 enzymatic activity

CPT2 activity was measured with parental wildtype and RR U251 cells collected in homogenization buffer (150 mM KCL, 5 mM Tris, pH 7.2) followed by 3 s sonication on ice. Then, 200 μg proteins were prepared in 200 μl final volume of homogenization buffer and 20 μl of 20% n-octyl-β-d-glucopyranoside was added into each sample and incubated on ice for 30 min with vortex every 5 min. To initiate reaction, 100 ml of homogenate was transferred into a mixture containing 100 μl of substrate mixture (0.2 mM l-carnitine, 0.013 μCi [¹⁴C]-carnitine, 0.05 mM palmitoyl-CoA), 700 μl assay cocktail (1.3% BSA, 2 mM KCN, 4.73 mM ATP, 4.2 mM MgCl2, 244 μM glutathione, 100 mM rotenone, and 0.15 M Tris buffer, pH 7.4) and 100 μM malonyl coenzyme (CPT1 fraction bio-inhibitor). The blank was prepared with 200 μl of 0.15 M KCL, 700 μl of assay cocktail, and 1 μl of 1.2 M HCl. The reaction mixtures were incubated at 30 °C for 30 min and followed with vortex for 15 sec and terminated by 1 M HCl. 1 mL of 1-butanol was then added, and the sample was centrifuged (1000 × g, 5 min at room temperature) to extract palmitoyl-carnitine (the top layer). About 500 μl of the top layer solution was transferred into a new tube along with 100 μl of H₂O followed by centrifugation (1000 × g, 5 min, room temperature), 200 μl of the top layer solution was transferred into scintillation vials with 4 ml of scintillation cocktail and counted in a Multi-Purpose Scintillation Counter (Beckman). Each sample was measured for 5 min, and the results were normalized by the corresponding protein concentrations.

Oil red O staining and quantitation

To evaluate the level of lipid accumulation in RR GBM cells, the oil red staining method was applied to WT versus RR U87 and U251 cells. In total, 3 × 10⁴ cells were seeded in each well of 96-well plates. After incubation for 12 h, cells were treated with 250 μM free FA oleate (oleic acid: palmitate acid = 2:1) for 24 h. Cells were rinsed with PBS twice and were fixed with 4% paraformaldehyde for 30 min at room temperature followed by washing with dH₂O twice. About 50 μl of Oil red working solution (oil red o stock solution: dH₂O at ratio 6:4) was added in dark for 15 min incubation at room temperature. Then the Oil Red O solution was removed and 50 μl of 60% isopropanol was added immediately in each well for 20 s at room temperature. Cells were washed 2–5 times with dH2O until excess stains were moved completely. The oil red dye was eluted with 100 μl DMSO and incubated for 10 min with gently shaking. The lipid accumulation results were detected using the fluorescence microplate spectrophotometer (Molecular Devices) at 510 nm. Images were obtained under a Nikon microscope (Eclipse, E1000M, Japan).

THP1 phagocytosis assay

In vitro phagocytosis was conducted using the human monocyte THP1 cells that were differentiated into macrophages by incubation with 40 nM Phorbol 12-myristate 13-acetate (PMA) for 48 h. The human GBM cell lines were stably transfected with GFP. GBM-GFP cells (1–1.5 × 10⁶⁾ were cocultured with macrophage (1 × 10⁶) per well in six wells plate for 2–4 h at 37 °C. Cocultured cells were collected and washed with 0.5% BSA-PBS. Cell pellets were incubated with fluorescence APC/Cyanine 7 labeled CD11b primary antibody (Catalog #101225, BioLegend) in dark for 30 min, followed by three times washing with 0.5% BSA-PBS. The phagocytic activity was analyzed by flow cytometry (Becton Dickinson canto II, BD, NJ, USA) and FlowJo (Three Star, Inc., Ashland, OR, USA).

Mouse bone marrow-derived macrophages

Bone marrow cells were collected from the femur of mice and passed through a 70 µm cells strainer. The cell suspension was then centrifuged at 1500 rpm for 5 min. The supernatant was discarded, and cells were then seeded into a six-well plate cultured for 7 days in a macrophage complete medium (DMEM/F12 growth medium, 10%FBS, 10mM l-glutamine, and 100 U/ml M-CSF). On day 3, cells were fed with another 1 ml macrophage complete medium. On day 7, cells were washed with PBS and stimulated with a macrophage complete medium containing lipopolysaccharide (LPS, 20 ng/ml) for 48 h.

Mouse bone marrow-derived macrophage phagocytosis

In vitro phagocytosis of mouse GBM cells was conducted using primary mouse bone marrow-derived macrophages that were differentiated into macrophages by incubation with 20 ng/ml LPS for 48 h. GFP-labeled tumor cells (GL261 cells, 1–1.5 × 10⁶) were cocultured with macrophage (1 × 10⁶) for 2–4 h at 37 °C. Cocultured cells were collected and washed with 0.5% BSA-PBS. Cell pellets were incubated with anti-CD11b (APC-Cy7) antibody in dark for 30 min, followed by three times washing with 0.5% BSA-PBS. The phagocytic activity was analyzed by flow cytometry (Becton Dickinson canto II, BD, NJ, USA) and FlowJo (Three Star, Inc., Ashland, OR, USA).

Immunocytochemistry (ICC)

To identify the co-expression of CD47 and CPT1A, CPT2, or ACAD9 in RR U251 cells, cells grown on round coverslips with 60–80% confluence were washed one time with PBS and fixed in 4% paraformaldehyde (pH 7. 2) for 20 min. After three times washing with PBS, cells were permeabilized with 0.2% Triton X-100 in PBS incubation at room temperature for 5 min. Samples were then blocked for 30 min with 1% BSA and co-incubated with primary antibodies derived from different species (anti-mouse CD47 or anti-rabbit CPT1A, CPT2, or ACAD9) at 1:50–250 dilutions overnight at 4 °C. Followed by three times washing with PBS, secondary antibodies (anti-mouse conjugated with Alex 488 or anti-Rabbit conjugated with APC) were added for incubation. Appropriated dilution (1:500 in 5% BSA) was selected and cells were incubated for 1 h at room temperature. Images were obtained, and the fluorescence intensity was quantified using fluorescent microscopy using Axiovision software (Zeiss, Germany). Positive cells were quantified by Image-Pro Plus 6.0 (Media Cybernetics, MD, USA).

RT-qPCR

Total RNA was extracted from cells using the Trizo method (Invitrogen). cDNA was synthesized by Superscript III first-strand cDNA synthesis kit (Invitrogen) according to the manufacturer’s instruction. RT-qPCR was performed with the SYBR Green PCR master mix (Thermo Fisher Scientific) on a 7500 Fast Real-Time PCR machine (Applied Biosystems/Thermo Fisher Scientific). The following primers were used:

hCD47 forward: 5′AGAAGGTGAAACGATCATCGAGC3′

hCD47 reverse: 5′CTCATCCATACCACCGGATCT3′

GAPDH forward: 5′ GGACTCATGACCACAGTCCAT 3′

GAPDH reverse: 5′ GTTCAGCTCAGGGATGACCTT 3′

β-actin forward: 5′CATGTACGTTGCTATCCAGGC3′

β-actin reverse: 5′CTCCTTAATGTCACGCACGAT3′.

Each sample was repeated in triplicate settings independent three times. Relative gene expression was calculated after correction for GAPDH and β-actin expression using the 2^−ΔΔCt method.

Luciferase reporter assay

Luciferase reporter (pGL2-basic-CD47) driven by human CD47 promotor and the control reporter with NF-κB binding sequence (769-757: GTGGAAGCTCCCT) deleted (pGL2-basic-CD47-ΔNF-κB) were constructed. The sequence deletion was conducted with Pfu Turbo DNA polymerase (Stratagene, La Jolla, CA) with the following PCT primers: CD47 NF-κB Delta F: 5′GTGGTCGGGTACCTGCCCGCTCGCCCCTCGCGGGCTCTGCG3′, CD47 NF-κB Delta R: 5′CGCAGAGCCCGCGAGGGGCGAGCGGGCAGGTACCCGACCAC3′. For transactivation analysis, GBM cells with or without FAO gene knockout (WT, CPT1A^−/− CPT2^−/− ACAD9^−/−) were seeded in triplicate 96-well plate (8000 cells/well) and co-transfected PolIII-Renilla control reporter with control NF-κB luciferase reporter, pGL2-basic-CD47 or pGL2-basic-CD47-ΔNF-κB for 48 h using transfection reagent TurboFect (Thermo Scientific, USA, catalog #R0533). Different treatments (citrate or SB204990) were conducted with the reporter-transfected WT GBM cells for varied time periods and cell lysates were prepared by adding 100 µl of lysis buffer (DTT 1 mM, MgCl₂ 8 mM, EGTA 4 mM, PMSF 100 nM) and incubated for 15 min at room temperature followed by adding 10 μl of lysates with 50 µl of luciferase buffer (5 mM DTT, 100 nM ATP, 150 µg/ml CoA) and 500 nM luciferin. The luciferase activity was measured using the Turner TD20/20 with Renilla luciferase for normalization transfection efficiency.

CRISPR/Cas9-mediated FAO gene edition

The Lenti-CRISPRv2 vector was purchased from the Addgene plasmid repository (Addgene cat #52961) (https://www.addgene.org). Single guide RNAs were designed using CRISPR design software (http://crispr.mit.edu) from Zhang’s lab. Oligos corresponding to the sgRNAs were synthesized and cloned into a lenti-CRISPRv2 vector following the protocol from Addgene. The human sgRNA sequences are shown as follow:

hCPT1A F: CACCGCTCCGGACGGGATTGACCTG

hCPT1A R: AAACCAGGTCAATCCCGTCCGGAGC

hCPT2 F: CACCGCGGGGCCCCGCGGTTGGTCC

hCPT2 R: AAACGGACCAACCGCGGGGCCCCGC

hACAD9 F: CACCGCTTGCCTAAACTGGCGTCCG

hACAD9 R: AAACCGGACGCCAGTTTAGGCAAGC

sgRNA sequencing primer: GAGGGCCTATTTCCCATGATT

Lentiviral particles were packaged in HEK293T cells according to the protocol from Addgene. GBM cells were plated at 1.25 × 10⁵ per well in 12-well plates. After 16 h, the medium was replaced with 0.5 ml lentiviruses and 0.5 ml fresh medium containing 10 ng polybrene (Sigma-Aldrich, Catalog #H9268-10G) for 6–8 h and then add an additional 0.5 ml fresh medium. After 12 h incubation, cells were exchanged to fresh medium and kept culturing for another 48 h. The infected cells were selected with 0.3 μg/ml puromycin for 1 week and confirmed by Western blot.

Cycloheximide chase assay

Chase assay was performed following the published protocols⁹¹. Briefly, 5 × 10⁶/well RR U251 cells were seeded into a six-well plate and after overnight incubation, the cultured medium was replaced by a fresh cell culture medium with or without A-485 (20 μM, 24 h). Cells were then treated with 150 μg/ml cycloheximide and time-dependent changes in the level of CD47 were analyzed by Western blotting.

Citrate detection

Cellular citrate concentration was measured by using the EnzyChromTM citrate Assay kit (Cat #ECIT-100, BioAssay Systems, Hayward, CA, USA). Briefly, 2 × 10⁶ cells were homogenized in 100 µl PBS and centrifuged at 14,000 rpm for 5 min and the clear supernatants were collected as the citrate sample. The standards of citrate were prepared by diluting citrate in dH₂O with a concentration range from blank to 400 μM. For detection of citrate concentration, the working reagent mix containing 85 µl Developer, 1 µl CL Enzyme, 1 µl ODC Enzyme, and 1 µl Dye was added into 20 μl of each standard or sample well. The sample without CL Enzyme was served as blank control. Citrate concentration was measured by using the fluorescence microplate spectrophotometer (Molecular Devices) at 570 nm and results were calculated as [Citrate] = R(sample) – R(blank /Slope (μM⁻¹) × n (μM), n = dilution factor.

Apoptosis analyses

Apoptotic cell death was measured in cells with different treatments or with the CRISPR knockout of FAO genes (CPT1A, CPT2, or ACAD9). Briefly, cells (2.5 × 10⁵) were seeded in a six-well plate and cultured overnight followed by 5 Gy irradiation treatment. The irradiated cells were further cultured for 48 h, and the attached cells and cells suspended in the medium were all collected and washed twice with 1× binding buffer (10 mM HEPES/NaOH, pH 7.4, 150 mM NaCl, 5 mM KCl, 1 mM MgCl₂, 1.8 mM CaCl₂). Cells were stained using Annexin-V/PI kit (Biosource, Invitrogen, Carlsbad, CA, USA) and apoptotic cells were analyzed by flow cytometry (Becton Dickinson canto II, BD, NJ, USA). Data were analyzed using Flowjo software (Three Star, Inc., Ashland, OR, USA).

Preparation for anti-mouse CD47 antibodies

Protein G Resin from GenScript (L00209) was applied for the preparation and purification of mammalian monoclonal and polyclonal IgG. Following standard column purification procedures, rat anti-mouse CD47 antibody IgG2α was purified from the supernatants of hybridoma MIAP301. The purified IgG was then concentrated using Protein Concentrator PES (88516) from Pierce. The final antibody concentration was determined using the absorbance at OD₂₈₀ by NanoDrop ND-1000 spectrophotometer.

Syngeneic subcutaneous mouse GBM tumor model

The animal use and care protocol applied in this test including local tumor radiation and ET and antibody treatment was approved by the Institutional Animal Use and Care Committee of the University of California Davis (IACUC 15315). For the test of palmitate (PA) consumption rate in RR GBM tumors, syngeneic mouse GBM tumors were subcutaneously generated with RR GL261 cells (5 × 10⁶ 100 μl PBS) inoculated into 6 weeks old female C57BL/6 mice (Taconic) in both flanks (n = 10; total tumor number = 20). When tumor volumes reached ~200 mm³, PA was intratumorally administrated (100 μl, 25 μM) for two treatments, one day apart, with 100 μl of 0.05% BSA-PBS injected into the other tumor of the same mouse used as the solvent control. Tumor volumes were calculated as V = Length × width²/2.

Human GBM tumors regrown model

To detect the enhancement of FAO enzymes and CD47 in human regrown tumors after radiation, 6 weeks old female nude mice (n = 4; The Jackson Laboratory, Stock No: 002019) were applied as the tumor-bearing animal with 5 × 10⁶ WT U251 cells inoculated subcutaneously in the right flanks of mice. When tumor volumes reached ~200 mm³ radiation treatment was delivered (2 Gy/day for continuously 5 days; total dose = 10 Gy). Reduction of tumor volumes were detected after radiation and regrown to about 200 mm³ by day 28. The tumors without radiation treatment were applied as the control. Expression of FAO enzymes and CD47 in control and regrown tumors was detected by Western blot and IHC staining.

Mouse orthotopic GBM regrown model

Mouse orthotopic tumor was established using WT GL261 cells transduced with lentivirus vector pCCLc-MNDU3-LUC-PGK-EGFP-WPRE following the reported protocol. Briefly, C57BL/6 mice (female, 6 weeks) from Taconic were anesthetized with ketamine (70 mg/kg) and xylazine (12 mg/kg). An incision longitudinally in the middle of the scalp was made followed by drilling the parietal bone to generate a 4 μm diameter hole located 1.5 mm posterior to the bregma and 1.5 mm to the right of the sagittal suture. GL261 cells (2.5 × 10⁵) in 5 μl PBS were intracranially injected through an entry site to a depth of 2.5 mm by a 10 μl Hamilton syringe. On day 10 when tumor image was detected, all mice were divided into two groups with or without radiation (n = 5). Radiation was delivered under anesthesia with 3 Gy on days 10, 12, and 14 and the control group received anesthesia without radiation. The tumor growth was in vivo monitored with BioSpec 7 T MRI scanner (Bruker, German) on day 10, day 17, and day 28, respectively after cell inoculation. The irradiated group was terminated at day 28 and the control group was ended at day 17 when tumor sizes reached the maximal.

Inhibition of orthotopic tumors regrown by blocking FAO and CD47

The mouse GBM orthotopic tumors regrown after radiation was used to investigate the synergetic effect of radiation combined with anti-CD47 antibody, etomoxir (ET), or a combination. When tumors were detected by in vivo imaging on Day 10, all tumors were irradiated with 3 Gy/day, every other day, for three times, total radiation dose = 9 Gy. Then mice were divided into four groups for further treatment starting on Day 24 as Group 1, IgG 16 mg/kg (n = 5); Group 2, anti-CD47 antibody 16 mg/kg (n = 5), Group 3, ET 30 mg/kg (n = 5); Group 4, combination anti-CD47 antibody and ET (n = 7). All reagents were intraperitoneally administrated every 2 days for a course of 2-week treatments with tumor monitored by in vivo imaging. Mice weight and clinical symptoms were observed and recorded every other day until the endpoint of the experiment. The whole-brain tissues were collected once euthanasia criteria were reached⁹² for the analysis of antibody penetration and macrophage-mediated phagocytosis.

CD47 antibody binding and macrophage-mediated phagocytosis

For immunofluorescence staining, orthotopic GFP expressing GBM model-derived tumor tissues were fixed in 4% paraformaldehyde and FFPE sectioned at 5 μm thickness were prepared. Antigen retrieval was applied in citrate buffer (pH 6.1) at 95 °C for 40 min in microwave and then flush dH₂O. Slides were blocked in 10% normal horse serum for 1 h at room temperature and then incubated with a primary antibody cocktail of Rabbit anti-rat IgG (1:500) and Mouse anti-GFP IgG (1:1000) with 10% normal horse serum. After washing three times with 1% BSA in PBS, slides were incubated with a secondary antibody cocktail of Alexa Fluor 488 conjugated Goat anti-mouse IgG and Rhodamine Red-X-Conjugated goat anti-rabbit (1:500 diluted in 10% normal horse serum) followed by a quick 1% Sudan black staining. Cell nuclei were counterstained using Vectashield Antifade solution containing DAPI (Vector Laboratories, Burlingame, CA, USA). For detection of macrophage-mediated phagocytosis, the primary antibody cocktail contained anti-GFP (1:100; cell signaling) and anti-CD11b (1:100; Millipore) and the secondary antibodies were 1:250 diluted fluorescent conjugated (anti-mouse-APC for CD11b and anti-rabbit-Alexa Fluor 488 for GFP). Fluorescent 14-bit images were obtained with the Zeiss Axioscan.Z1 slide scanning system (Carl Zeiss Microscopy, Oberkochen, Germany) using a Plan-Apochromat 20x/0.8 M27 objective with standard acquisition settings. The light source intensity and exposure time per dye were described as below (Table 1):

Table 1 CD47 antibody binding and macrophage-mediated phagocytosis.

Full size table

The positive cells were quantified by Image-Pro Plus 6.0. The phagocytic index was calculated by the following formula: Phagocytic index = (number of engulfed cells/total number of macrophages containing engulfed cells) × (total number of macrophages containing engulfed cells/total number of counted macrophages) × 100.

Quantification and statistical analysis

Data generated by in vitro and in vivo mouse tumor models in this study are presented as mean ± SD and analyzed using the two-tailed Student t-test for two groups or ANOVA for multiple groups. The statistical significance of Kaplan–Meier survival curves was assessed with a Mann–Whitney test. A value of P less than 0.05 was considered statistically significant. *P < 0.05, **P < 0.01, ***P < 0.001, and **** P < 0.0001.

Reporting summary

Further information on research design is available in the Nature Research Reporting Summary linked to this article.

Data availability

The RNA sequencing data referenced during the study are available in a public repository (Part B, DataSet ID: mRNAseq_693) from CGGA website (http://www.cgga.org.cn/download/20191128/download.jsp).

The data that support the findings of this study are within the Article, Supplementary Information, or available from the corresponding author upon reasonable request. Source data are provided with this paper. Requests for resources and reagents should be directed to Jian Jian Li (jijli@ucdavis.edu). Source data are provided with this paper.

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Acknowledgements

We acknowledge Dr. Irving Weissman for providing the anti-human CD47 antibody Hu5F9-G4, Dr. William Frazier for providing anti-mouse CD47 MIAP301 hybridoma, Dr. Watkins Vockley for providing anti-ACAD9 antibody, Dr. Orin Bloch for discussion of in vivo mouse GBM model, and Dr. Ji Ming Wang for reading the manuscript. The clinical and pathological studies conducted in Xiangya Hospital, Central South University, were supported by the National Foundation of Natural Science (81672509). Dr. Nian Jiang was supported by China Scholarship Council (201706370117). The radioresistant GBM cell lines and in vitro and in vivo studies using radioresistant mouse GBM cells carried out at the Cancer Center of University of California Davis were supported by NCI RO1 CA213830 (J.J.L.), NCI R01 CA224900 (H.-W.C.), VA MERIT Award I01BX004271 (H.-W.C.), UC Davis Immunology Pilot Grant Support (J.J.L.), and UC Davis Cancer Center supported by the CCSG Grant awarded by the National Cancer Institute (Dr. Primo Lara; NCI P30CA093373). This work is dedicated to the memory of Dr. William C. Dewey for his mentoring and long-term friendship. “A mentor is someone who sees more talent and ability within you, than you see in yourself, and helps bring it out of you”—Bob Proctor.

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Authors and Affiliations

Department of Radiation Oncology, University of California Davis School of Medicine, Sacramento, CA, 95817, USA
Nian Jiang, Bowen Xie, Ming Fan, Yixin Duan, Angela C. Evans, Jie Huang, Weibing Zhou, Wen Chen, Ruben C. Fragoso, Paul F. Wilson, Matthew A. Coleman, Arta M. Monjazeb & Jian Jian Li
Department of Neurosurgery, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, PR China
Nian Jiang, Xuelei Lin, Ningrong Ye, Siyi Wanggou & Xuejun Li
Institute for Immunology and School of Medicine, Tsinghua University, Beijing, 100084, PR China
Bowen Xie
Department of Biochemistry and Molecular Medicine, University of California Davis, Sacramento, CA, 95817, USA
Wenwu Xiao, Di Jing, Hsing-Jien Kung, Kit S. Lam & Hong-Wu Chen
Department of Surgery, School of Medicine, University of California Davis, Sacramento, CA, 95817, USA
Shanxiu Xu & Aijun Wang
Department of Pathology and Laboratory Medicine, University of California Davis, Sacramento, CA, 95817, USA
Yamah Hamsafar & Brittany N. Dugger
Department of Radiation Oncology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, PR China
Weibing Zhou, Wen Chen & Di Jing
NCI-Designated Comprehensive Cancer Center, University of California Davis, Sacramento, CA, 95817, USA
Ruben C. Fragoso, Paul F. Wilson, Matthew A. Coleman, Arta M. Monjazeb, William J. Murphy, Kit S. Lam, Hong-Wu Chen & Jian Jian Li
Department of Neurology, Johns Hopkins School of Medicine, Baltimore, MD, 21205, USA
Shuli Xia
Hunan International Scientific and Technological Cooperation Base of Brain Tumor Research, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, PR China
Xuejun Li
Center for Molecular Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, PR China
Lun-Quan Sun
Departments of Dermatology and Internal Medicine, UC Davis School of Medicine, Sacramento, CA, 95817, USA
William J. Murphy
TMU Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei, 110, Taiwan
Hsing-Jien Kung
Veterans Affairs Northern California Health Care System, Mather, CA95655, USA
Hong-Wu Chen

Authors

Nian Jiang
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Bowen Xie
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Wenwu Xiao
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Ming Fan
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Jie Huang
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Paul F. Wilson
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Shuli Xia
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Xuejun Li
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Contributions

N.J., B.X., S.X., L.-Q.S., Xj.L. A.M.M, W.J.M., H.-J.K., K.S.L., H.-W.C., and J.J.L. conceived the project. N.J., B.X., W.X., M.F., J.H., W.Z., P.F.W., M.A.C., and A.W. conducted experiments on in vitro and in vivo investigation. N.J., M.F., Y.H., Y.D., A.C.E., Sh.X., and B.N.D. conducted on in vitro and in vivo imaging analysis. R.C.F., X.L., Xj.L., N.Y., S.W., W.C., and D.J. conducted clinical sampling and analysis. X.L, N.Y., and S.W conducted on bioinformatics. N.J., B.W., Sh.X., K.S.L., H.-W.C., and J.J.L. wrote the manuscript. All authors commented on the manuscript.

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Correspondence to Jian Jian Li.

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Jiang, N., Xie, B., Xiao, W. et al. Fatty acid oxidation fuels glioblastoma radioresistance with CD47-mediated immune evasion. Nat Commun 13, 1511 (2022). https://doi.org/10.1038/s41467-022-29137-3

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Received: 29 January 2021
Accepted: 25 February 2022
Published: 21 March 2022
DOI: https://doi.org/10.1038/s41467-022-29137-3

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