Abstract
Background
Advanced prostate cancer (PCa) is often resistant to immunotherapy. In this study, we examined the role of CD276 in mediating immunotherapeutic effects through changes in immune cell infiltration.
Methods
Using transcriptomic and proteomic analyses, CD276 was identified as a potential target for immunotherapy. Subsequent in vivo and in vitro experiments confirmed its role as a potential mediator of immunotherapeutic effects.
Results
Multi-omic analysis suggested that CD276 was identified as a key molecule regulating the immune microenvironment (IM). In vivo experiments revealed that CD276 knockdown was found to enhance CD8+ T cell infiltration into the IM. Immunohistochemical analysis of PCa samples further confirmed the same findings.
Conclusion
CD276 was found to inhibit the enrichment of CD8+ T cells in PCa. Thus, CD276 inhibitors may be potential targets for immunotherapy.
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Data availability
All public database sources can be found in MATERIALS AND METHODS. And the Clinical data from STPH can be found in supplementary material.
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Acknowledgements
We would like to thank Prof. Qiangyuan Fan for excellent technical support.
Funding
This work was supported in part by a grant from the National Natural Science Foundation of China, Youth Project (#82101838,#82203367); Experimental Animal Fund of Shanghai Science and Technology Commission, (#22140903800).
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LJ and AJ wrote the manuscript. GYD, ZWT, and CHT collected patient data from our center. ZZJ, WH, and ZY contributed to data analysis, and BMY and GJ contributed to the in vivo experiment. MSY, ZJF, and YXD contributed ideas of the manuscript. All authors read and approved the final manuscript.
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The authors declare no competing interests.
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All patients gave informed consent to the study, which was approved by the ethics committee of Shanghai Tenth People’s Hospital (No: SHSY-IEC-4.1/19-120/01).
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Liu, J., Kadier, A., Guo, Y. et al. Effect of tumor CD276 expression on infiltrating immune cells and clinicopathological features of prostate cancer. Prostate Cancer Prostatic Dis (2023). https://doi.org/10.1038/s41391-023-00690-2
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DOI: https://doi.org/10.1038/s41391-023-00690-2