In their article (Microglia in neurodegenerative disease. Nat. Rev. Neurol. 6, 193–201; 2010)1 Perry et al. provide a comprehensive review of the involvement of microglia in neurodegenerative disease. The authors highlight the substantial interest that exists regarding the possible roles of activated microglia in disease pathogenesis, and question whether activated microglia exacerbate pathology or aid tissue repair in neurodegenerative diseases. In all of the diseases used as examples by the authors, microglial activation is secondary to other pathological processes that affect the CNS. In order to contribute to this discussion, we would like to highlight the interesting example of Nasu–Hakola disease, and discuss how microglial dysfunction might cause substantial brain damage.2,3,4,5,6 This example of microglial dysfunction causing brain damage is important because it might offer insights into the importance of microglial homeostasis in the brain.
Nasu–Hakola disease—also known as polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL)—is a genetic disorder characterized by progressive presenile dementia and bone cysts.2,3,4,5,6,7 Patients with Nasu–Hakola disease develop a variant of frontotemporal dementia associated with cyst-like bone lesions (Figure 1). The disease usually starts during the 4th decade of life, leading to death after only a few years.2,3,4,5,6,7 In contrast to other diseases, evidence strongly suggests that in Nasu–Hakola disease CNS damage is directly caused by microglial dysfunction.2,3,8,9 Histological examination of brains from patients with Nasu–Hakola disease shows that neuronal loss, and astrocyte proliferation and hypertrophy, occurs mainly in the white matter of the frontotemporal lobe and basal ganglia.3,10 Nevertheless, the most interesting feature of this disease is a well-documented activation of microglia in the cerebral white matter of patients with this condition, and this prominent microglial activation might be the primary underlying factor responsible for Nasu–Hakola disease.3,11
Genetic studies have shown that mutations in the genes TREM2 and TYROBP, which encode the proteins TREM2 and TYROBP, respectively, are responsible for the phenotypic abnormalities in homozygous patients with this condition.5,12 The TREM2–TYROBP protein complex regulates the differentiation and function of osteoclasts, which are bone-resorbing cells.6,13 In the brain, TREM2–TYROBP has been shown to be expressed mainly—or even exclusively—by microglia, and is thought to regulate cell function.3,7,10,14,15 Takahashi et al. showed that knockdown of TREM2 in microglia inhibited phagocytosis of apoptotic neurons and increased proinflammatory responses,15 a finding important for understanding Nasu–Hakola disease as well as other CNS diseases. Although all TREM2–TYROBP functions have not been fully elucidated, Nasu–Hakola disease might be an interesting example of how primary microglial dysfunction can damage the CNS.
Nasu–Hakola disease is emerging as the prototype of a primary microglial disorder of the CNS.2,8 Whether Nasu–Hakola disease is a unique disorder, or if this condition is the first identified disorder in an entirely new class of diseases that one could eventually call 'microgliopathies', is unknown. Clinical observations of patients with Nasu–Hakola disease and molecular studies on the TREM2–TYROBP pathway might offer interesting insights into microglial physiology and the involvement of this cell type in neurodegenerative disorders. We believe that pharmacological modulation of the TREM2–TYROBP pathway might offer new therapeutic strategies for the treatment of neurological diseases.
References
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The authors are supported by Brazilian governmental research grants (CNPq, FAPERGS and Propesq/UFRGS).
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Bianchin, M., Martin, K., de Souza, A. et al. Nasu–Hakola disease and primary microglial dysfunction. Nat Rev Neurol 6, 523 (2010). https://doi.org/10.1038/nrneurol.2010.17-c1
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DOI: https://doi.org/10.1038/nrneurol.2010.17-c1
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