Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Limitations and possibilities of small RNA digital gene expression profiling

This is a preview of subscription content, access via your institution

Relevant articles

Open Access articles citing this article.

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Figure 1: microRNA expression profiles from different sequencing platforms.
Figure 2: DGE profiling analysis of a synthetic RNA pool.

References

  1. Berezikov, E., et al. Genome Res. 16, 1289–1298 (2006).

    Article  CAS  Google Scholar 

  2. Ruby, J.G. et al. Cell 127, 1193–1207 (2006).

    Article  CAS  Google Scholar 

  3. Kuchenbauer, F. et al. Genome Res. 18, 1787–1797 (2008).

    Article  CAS  Google Scholar 

  4. Berezikov, E. et al. Nat. Genet. 38, 1375–1377 (2006).

    Article  CAS  Google Scholar 

  5. Lau, N.C., Lim, L.P., Weinstein, E.G. & Bartel, D.P. Science 294, 858–862 (2001).

    Article  CAS  Google Scholar 

  6. Griffiths-Jones, S., Grocock, R.J., van Dongen, S., Bateman, A. & Enright, A.J. Nucleic Acids Res. 34, D140–D144 (2006).

    Article  CAS  Google Scholar 

  7. Romaniuk, E., McLaughlin, L.W., Neilson, T. & Romaniuk, P.J. Eur. J. Biochem. 125, 639–643 (1982).

    Article  CAS  Google Scholar 

  8. Taube, R., Loya, S., Avidan, O., Perach, M. & Hizi, A. Biochem. J. 329, 579–587 (1998).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the Cancer Genomics Center and Netherlands Bioinformatic Center through Netherlands Genomics Initiative (to E.C.), Fred Hutchinson Cancer Research Center and Canary Foundation funding (New Development funds to M.T.), Core Center of Excellence in Hematology Pilot Grant P30 DK56465 (to M.T.), Pacific Northwest Prostate Cancer Specialized Program of Research Excellence Grant P50 CA97186 (to M.T.) and a Rosetta Inpharmatics Fellowship in Molecular Profiling (to S.K.W.). We thank P. Toonen for animal care.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Edwin Cuppen.

Ethics declarations

Competing interests

S.K. is an employee of Life Technologies. Various products of Life Technologies were used in experiments for this manuscript.

Supplementary information

Supplementary Text and Figures

Supplementary Figures 1-6, Supplementary Note and Supplementary Methods (PDF 807 kb)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Linsen, S., de Wit, E., Janssens, G. et al. Limitations and possibilities of small RNA digital gene expression profiling. Nat Methods 6, 474–476 (2009). https://doi.org/10.1038/nmeth0709-474

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nmeth0709-474

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing