Abstract
The ability of Saccharomyces cerevisiae to tolerate ionizing radiation damage requires many DNA-repair and checkpoint genes, most having human orthologs. A genome-wide screen of diploid mutants homozygous with respect to deletions of 3,670 nonessential genes revealed 107 new loci that influence γ-ray sensitivity. Many affect replication, recombination and checkpoint functions. Nearly 90% were sensitive to other agents, and most new genes could be assigned to the following functional groups: chromatin remodeling, chromosome segregation, nuclear pore formation, transcription, Golgi/vacuolar activities, ubiquitin-mediated protein degradation, cytokinesis, mitochondrial activity and cell wall maintenance. Over 50% share homology with human genes, including 17 implicated in cancer, indicating that a large set of newly identified human genes may have related roles in the toleration of radiation damage.
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Acknowledgements
We thank J. Marks, K. Bloom, D. Gordenin, Y. Pavlov and T. Darden for critical comments and suggestions on the manuscript. The efforts of the consortium responsible for the yeast gene deletion project is greatly appreciated. We thank J. Haber for plasmid pJH132.
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Bennett, C., Lewis, L., Karthikeyan, G. et al. Genes required for ionizing radiation resistance in yeast. Nat Genet 29, 426–434 (2001). https://doi.org/10.1038/ng778
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DOI: https://doi.org/10.1038/ng778
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