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A protein linkage map of Escherichia coli bacteriophage T7

Nature Genetics volume 12, pages 72–77 (1996)Cite this article

Abstract

Genome sequencing projects are predicting large numbers of novel proteins, whose interactions with other proteins must mediate the function of cellular processes. To analyse these networks, we used the yeast two-hybrid system on a genome-wide scale to identify 25 interactions among the proteins of Escherichia coli bacteriophage T7. Among these is a set of six interactions connecting proteins that function in DMA replication and DMA packaging. Remarkably, two genes, arranged such that one entirely overlaps the other and uses a different reading frame, encode interacting proteins. Several of the interactions reflect intramolecular associations of different domains of the same polypeptide, suggesting that the two-hybrid assay may be useful in the analysis of protein folding. This global approach to protein–protein interactions may be applicable to the analysis of more complex genomes whose sequences are becoming available.

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Author notes

  1. Paul L. Bartel

    Present address: Myriad Genetics, Inc., 390 Wakara Way, Salt Lake City, Utah, 84108, USA

  2. Stanley Fields: Correspondence should be addressed to S.F. at the University of Washington.

Authors and Affiliations

  1. Department of Molecular Genetics and Microbiology, State University of New York at Stony Brook, Stony Brook, New York, 11794, USA

    Paul L. Bartel, Dhruba SenGupta & Stanley Fields

  2. Graduate Program in Molecular and Cellular Biology, State University of New York at Stony Brook, Stony Brook, New York, 11794, USA

    Jennifer A. Roecklein

  3. Departments of Genetics and Medicine, Markey Molecular Medicine Center, Box 357360, University of Washignton, Seattle, Washington, 98195, USA

    Dhruba SenGupta & Stanley Fields

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  4. Stanley Fields
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Bartel, P., Roecklein, J., SenGupta, D. et al. A protein linkage map of Escherichia coli bacteriophage T7. Nat Genet 12, 72–77 (1996). https://doi.org/10.1038/ng0196-72

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