Modelling organs in culture has great potential to improve our understanding of development, organogenesis and disease. While some endodermal derived organs have been modelled, the corpus region of the stomach, where acid-producing cells reside, remained an invincible target. A 60-day differentiation protocol now enables the generation of functional acid-producing cells in culture, conquering the challenge.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Noguchi, T. K. et al. Nat. Cell Biol. 17, 984–993 (2015).
Lee, E. R., Trasler, J., Dwivedi, S. & Leblond, C. P. Am. J. Anat. 164, 187–207 (1982).
Karam, S. M. & Leblond, C. P. Anat. Rec. 236, 259–279 (1993).
Ootani, A., Toda, S., Fujimoto, K. & Sugihara, H. Biochem. Biophys. Res. Commun. 271, 741–746 (2000).
Katano, T. et al. Biochem. Biophys. Res. Commun. 432, 558–563 (2013).
Li, X. et al. Nat. Med. 20, 769–777 (2014).
Barker, N. et al. Cell Stem Cell 6, 25–36 (2010).
Stange, D. E. et al. Cell 155, 357–368 (2013).
Bartfeld, S. et al. Gastroenterology 148, 126–136 (2015).
McCracken, K. W. et al. Nature 516, 400–404 (2014).
Zorn, A. M. & Wells, J. M. Annu. Rev. Cell Dev. Biol. 25, 221–251 (2009).
D'Amour, K. A. et al. Nat. Biotechnol. 23, 1534–1541 (2005).
Kubo, A. et al. Development 131, 1651–1662 (2004).
Fukuda, K. & Yasugi, S. Dev. Growth Differ. 47, 375–382 (2005).
Ramalho-Santos, M., Melton, D. A. & McMahon, A. P. Development 127, 2763–2772 (2000).
Torihashi, S. et al. Stem Cells 24, 2618–2626 (2006).
Suerbaum, S. & Michetti, P. N. Engl. J. Med. 347, 1175–1186 (2002).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Competing interests
The author declares no competing financial interests.
Rights and permissions
About this article
Cite this article
Huch, M. Building stomach in a dish. Nat Cell Biol 17, 966–967 (2015). https://doi.org/10.1038/ncb3211
Published:
Issue Date:
DOI: https://doi.org/10.1038/ncb3211
This article is cited by
-
Organoid culture to study epithelial cell differentiation and barrier formation in the colon: bridging the gap between monolayer cell culture and human subject research
In Vitro Cellular & Developmental Biology - Animal (2021)
-
Organoids as Model Systems for Gastrointestinal Diseases: Tissue Engineering Meets Genetic Engineering
Current Pathobiology Reports (2016)
