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Immunofluorescence stimulated emission depletion microscopy

Nature Biotechnology volume 21, pages 1303–1304 (2003)Cite this article

Abstract

We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of ∼50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We have demonstrated not only that an antibody-tagged label is stable enough to be recorded in this microscopy mode, but also that subdiffraction resolution can be obtained using a standard immunofluorescence preparation.

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Figure 1: Subdiffraction immunofluorescence imaging with STED-4Pi microscopy.

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Acknowledgements

We thank Lars Kastrup for valuable discussions and the German Ministry for Research and Education (BMBF) for partial support.

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Authors and Affiliations

  1. Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, 37070, Germany

    Marcus Dyba, Stefan Jakobs & Stefan W Hell

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  1. Marcus Dyba
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  2. Stefan Jakobs
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  3. Stefan W Hell
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Corresponding author

Correspondence to Stefan W Hell.

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The authors declare no competing financial interests.

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Dyba, M., Jakobs, S. & Hell, S. Immunofluorescence stimulated emission depletion microscopy. Nat Biotechnol 21, 1303–1304 (2003). https://doi.org/10.1038/nbt897

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  • DOI: https://doi.org/10.1038/nbt897

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