Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • On the Market
  • Published:

High-sensitivity immunofluorescence for detection of the pro- and anti-inflammatory cytokines gamma interferon and interleukin-10 on the surface of cytokine-secreting cells

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Surface staining with SaDIG liposomes or SaDIG–fluorescein.
Figure 2: Correlation of surface cytokine expression with cytokine secretion.
Figure 3: Staining of surface cytokines on cytokine-transfected cell lines.

References

  1. Shapiro, H. in Practical Flow Cytometry (Alan R. Liss, New York, 1988).

  2. Zola, H. Detection of receptors for cytokines and growth factors. Immunologist 2, 47–50 (1994).

    CAS  Google Scholar 

  3. Lassoued, K. et al. Expression of surrogate light chain receptors is restricted to a late stage in pre-B cell differentiation. Cell 73, 73–86 (1993).

    Article  CAS  Google Scholar 

  4. Bruno, L. et al. Threshold of pre-T-cell-receptor surface expression is associated with alpha/beta T-cell lineage commitment. Curr. Biol. 9, 559–568 (1999).

    Article  CAS  Google Scholar 

  5. Scheffold, A., Miltenyi, S. & Radbruch, A. Magnetofluorescent liposomes for increased sensitivity of immunofluorescence. Immunotechnology 1, 127–137 (1995).

    Article  CAS  Google Scholar 

  6. Assenmacher, M. et al. Specific expression of surface interferon-γ on interferon-γ producing T cells from mouse and man. Eur. J. Immunol. 26, 263–267 (1996).

    Article  CAS  Google Scholar 

  7. Chizzolini, C. et al. Inhibition of type I collagen production by dermal fibroblasts upon contact with activated T cells: different sensitivity to inhibition between systemic sclerosis and control fibroblasts. Arthritis Rheum. 41, 2039–2047 (1998).

    Article  CAS  Google Scholar 

  8. Rezzonico, R., Burger, D. & Dayer, J.M. Direct contact between T lymphocytes and human dermal fibroblasts or synoviocytes down-regulates types I and III collagen production via cell-associated cytokines. J. Biol. Chem. 273, 18720–18728 (1998).

    Article  CAS  Google Scholar 

  9. Hope, H.J. et al. Production of large unilamellar vesicles by a rapid extrusion procedure. Characterization of size distribution, trapped volume and ability to maintain a membrane potential. Biochim. Biophys. Acta 812, 55–65 (1985).

    Article  CAS  Google Scholar 

  10. Brotherick, I. et al. Flow cytometric method for the measurement of epidermal growth factor receptor and comparison with the radio-ligand binding assay. Cytometry 16, 262–269 ( 1994).

    Article  CAS  Google Scholar 

  11. Huang, S. et al. Immune response in mice that lack the interferon-gamma receptor . Science 259, 1742–1745 (1993).

    Article  CAS  Google Scholar 

  12. Lollini, P.-L. et al. Inhibition of tumor growth and metastasis after transfection of the gamma-interferon gene. Int. J. Cancer 55, 320–329 (1993).

    Article  CAS  Google Scholar 

  13. Richter, G. et al. Interleukin-10 transfected into Chinese Hamster Ovary cells prevents tumor growth and prevents macrophage infiltration. Cancer Res. 53, 4134–4137 ( 1993).

    CAS  PubMed  Google Scholar 

  14. Lee, F. et al. Isolation and characterization of a mouse interleukin cDNA clone that expresses B cell costimulatory factor 1 activities and T cell and mast cell stimulating activities. Proc. Natl. Acad. Sci. USA 83, 2061–2065 (1986).

    Article  CAS  Google Scholar 

  15. Karasuyama, H., Tohyama, N. & Tada, T. Autocrine growth and tumorigenicity of interleukin-2 dependent helper T cells transfected with IL-2 gene. J. Exp. Med. 169, 13–25 (1989).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank R. Christine, D. Kunkel and C. Raulfs for critical reading of the manuscript.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Alexander Scheffold.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Scheffold, A., Assenmacher, M., Reiners-Schramm, L. et al. High-sensitivity immunofluorescence for detection of the pro- and anti-inflammatory cytokines gamma interferon and interleukin-10 on the surface of cytokine-secreting cells. Nat Med 6, 107–110 (2000). https://doi.org/10.1038/71441

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/71441

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing