Nature Genetics
21, 10 - 14 (1999)
doi:10.1038/4434
Expression profiling using cDNA microarraysDavid J Duggan, Michael Bittner, Yidong Chen, Paul Meltzer
& Jeffrey M. Trent
Cancer Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.jtrent@nhgri.nih.gov
cDNA microarrays are capable of profiling gene expression patterns of
tens of thousands of genes in a single experiment. DNA targets, in the form
of 3´ expressed sequence tags (ESTs), are arrayed onto glass slides
(or membranes) and probed with fluorescent− or radioactively−labelled
cDNAs. Here, we review technical aspects of cDNA microarrays, including the
general principles, fabrication of the arrays, target labelling, image analysis
and data extraction, management and mining.
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