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Cloning and expression of apical membrane water channel of rat kidney collecting tubule

Abstract

CONCENTRATING urine is mandatory for most mammals to prevent water loss from the body. Concentrated urine is produced in response to vasopressin by the transepithelial recovery of water from the lumen of the kidney collecting tubule through highly water-permeable membranes1,2. In this nephron segment, vaso-pressin regulates water permeability by endo- and exocytosis of water channels from or to the apical membrane3,4. CHIP28 is a water channel in red blood cells and the kidney proximal tubule5, but it is not expressed in the collecting tubule6. Here we report the cloning of the complementary DNA for WCH-CD, a water channel of the apical membrane of the kidney collecting tubule. WCH-CD is 42% identical in amino-acid sequence to CHIP28. WCH-CD transcripts are detected only in the collecting tubule of the kidney. Immunohistochemically, WCH-CD is localized to the apical region of the kidney collecting tubule cells. Expression of WCH-CD in Xenopus oocytes markedly increases osmotic water permeability. The functional expression and the limited localization of WCH-CD to the apical region of the kidney collecting tubule suggest that WCH-CD is the vasopressin-regulated water channel.

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References

  1. Orloff, J. & Handler, J. S. Am. J. Med. 42, 757–768 (1967).

    Article  CAS  Google Scholar 

  2. Knepper, M. A. & Rector, F. C. Jr. in The Kidney (eds Brenner, B. M. & Rector, F. C. Jr) 445–482 (Saunders, Philadelphia, 1991).

    Google Scholar 

  3. Handler, J. S., Am. J. Physiol. 255, F375–F382 (1988).

    CAS  PubMed  Google Scholar 

  4. Harris, H. W. Jr, Strange, K. & Zeidel, M. L. J. clin. Invest. 88, 1–8 (1991).

    Article  CAS  Google Scholar 

  5. Preston, G. M., Carroll, T. P., Guggino, W. B. & Agre, P. Science 256, 385–387 (1992).

    Article  ADS  CAS  Google Scholar 

  6. Denker, B. M., Smith, B. L., Kuhajda, F. P. & Agre, P. J. biol. Chem. 263, 15634–15642 (1988).

    CAS  PubMed  Google Scholar 

  7. Saiki, R. K. et al. Science 239, 487–491 (1988).

    Article  ADS  CAS  Google Scholar 

  8. Wistow, G. J., Pisano, M. M. & Chepelinsky, A. B. Trends biochem. Sci. 16, 170–171 (1991).

    Article  CAS  Google Scholar 

  9. Kent, N. A. & Shiels, A. Nucleic Acids Res. 18, 4256 (1990).

    Article  CAS  Google Scholar 

  10. Pao, G. M. et al. Molec. Microbiol. 5, 33–37 (1991).

    Article  CAS  Google Scholar 

  11. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).

    Article  CAS  Google Scholar 

  12. Moriyama, T., Murphy, H. R., Martin, B. M. & Garcia-Perez, A. Am. J. Physiol. 258, F1470–F1474 (1990).

    CAS  PubMed  Google Scholar 

  13. Terada, Y. et al. Am. J. Physiol. 261, F1080–F1087 (1991).

    CAS  PubMed  Google Scholar 

  14. Zhang, R. & Verkman, A. S. Am. J. Physiol. 260, C26–C34 (1991).

    Article  CAS  Google Scholar 

  15. Verkman, A. S. A. Rev. Physiol. 54, 97–108 (1992).

    Article  CAS  Google Scholar 

  16. Dascal, N. CRC Crit. Rev. Biochem. 22, 317–373 (1987).

    Article  CAS  Google Scholar 

  17. Sigel, E. J. Membr. Biol. 117, 201–221 (1990).

    Article  CAS  Google Scholar 

  18. Kuwahara, M., Berry, C. A. & Verkman, A. S. Biophys. J. 54, 595–602.

  19. Preston, G. M. & Agre, P. Proc. natn. Acad. Sci. U.S.A. 88, 11110–11114 (1991).

    Article  ADS  CAS  Google Scholar 

  20. Kornfeld, R. & Kornfeld, S. A. Rev. Biochem. 54, 631–664 (1985).

    Article  CAS  Google Scholar 

  21. Kennelly, P. J. & Krebs, E. G. J. biol. Chem. 266, 15555–15558 (1991).

    CAS  PubMed  Google Scholar 

  22. Kozak, M. Nucleic Acids Res. 15, 8127–8146 (1987).

    Article  Google Scholar 

  23. Pisano, M. M. & Chepelinsky, A. B. Genomics 11, 981–990 (1991).

    Article  CAS  Google Scholar 

  24. Skowsky, W. R. & Fisher, D. A. J. Lab. clin. Med. 80, 134–144 (1972).

    CAS  Google Scholar 

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Fushimi, K., Uchida, S., Harat, Y. et al. Cloning and expression of apical membrane water channel of rat kidney collecting tubule. Nature 361, 549–552 (1993). https://doi.org/10.1038/361549a0

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