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Characterization of the rough endoplasmic reticulum ribosome-binding activity

Abstract

THE rough endoplasmic reticulum membranes of mammalian cells contain specific ribosome-binding sites1. A purification to apparent homogeneity of a negatively charged protein (ERpl80) of relative molecular mass 180,000 (180 K) was reported which was proposed to function as a rough endoplasmic reticulum ribosome receptor2. We report here that ribosome-binding site activity quantitatively solubilized from rough endoplasmic reticulum membranes does not cofractionate with ERpl80. By contrast, ribosome-binding site activity fractionates as a much smaller, positively charged protein.

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References

  1. Borgese, N., Mok, W., Kreibich, G. & Sabatini, D. D. J. molec. Biol. 88, 559–580 (1974).

    Article  CAS  Google Scholar 

  2. Savitz, A. J. & Meyer, D. I. Nature 346, 540–544 (1990).

    Article  ADS  CAS  Google Scholar 

  3. Hortsch, M., Avossa, D. & Meyer, D. I. J. Cell Biol. 103, 241–253 (1986).

    Article  CAS  Google Scholar 

  4. Yoshida, H. et al. Biochem. J. 259, 811–819 (1987).

    Article  Google Scholar 

  5. Collins, P. G. & Gilmore, R. J. Cell Biol. (in the press).

  6. Kreibich, G., Ulrich, B. L. & Sabatini, D. D. J. Cell Biol. 77, 464–487 (1978).

    Article  CAS  Google Scholar 

  7. Tazawa, S. J. Biochem. 109, 89–98 (1991).

    CAS  PubMed  Google Scholar 

  8. Walter, P. & Blobel, G. Meth. Enzym. 96, 682–691 (1983).

    Article  CAS  Google Scholar 

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Nunnari, J., Zimmerman, D., Ogg, S. et al. Characterization of the rough endoplasmic reticulum ribosome-binding activity. Nature 352, 638–640 (1991). https://doi.org/10.1038/352638a0

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  • DOI: https://doi.org/10.1038/352638a0

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