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High-affinity NGF binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor

Nature volume 350, pages 678–683 (1991)Cite this article

Abstract

Nerve growth factor (NGF) interacts with two different low-affinity receptors that can be distinguished by affinity crosslinking. Reconstitution experiments by membrane fusion and transient transfection into heterologous cells indicate that high-affinity NGF binding requires coexpression and binding to both the low-affinity NGF receptor and the tyrosine kinase trk gene product. These studies reveal a new growth factor receptor-mediated mechanism of cellular differentiation involving trk and the low-affinity NGF receptor.

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Author notes

  1. Dionisio Martin-Zanca

    Present address: Institute de Microbiologia Bioquimica, CSIC, Universidad de Salamanca, 37008, Salamanca, Spain

Authors and Affiliations

  1. Hematology/Oncology Division, Department of Medicine, Department of Cell Biology and Anatomy, Cornell University Medical College, 1300 York Avenue, New York, New York, 10021, USA

    Barbara L. Hempstead & Moses V. Chao

  2. Molecular Embryology Group, ABL-Basic Research Program, NCI-Frederick Cancer Research & Development Center, PO Box B, Frederick, Maryland, 21701, USA

    Dionisio Martin-Zanca & Luis F. Parada

  3. Eukaryotic Signal Transduction Group, ABL-Basic Research Program, NCI-Frederick Cancer Research & Development Center, PO Box B, Frederick, Maryland, 21701, USA

    David R. Kaplan

Authors
  1. Barbara L. Hempstead
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  2. Dionisio Martin-Zanca
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  3. David R. Kaplan
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  4. Luis F. Parada
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  5. Moses V. Chao
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Hempstead, B., Martin-Zanca, D., Kaplan, D. et al. High-affinity NGF binding requires coexpression of the trk proto-oncogene and the low-affinity NGF receptor. Nature 350, 678–683 (1991). https://doi.org/10.1038/350678a0

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