Abstract
A MAJOR effect of insulin is to increase glucose transport in muscle and fat1,2. A family of genes encoding distinct mammalian glucose transporters has recently been elucidated3–9. One of these, the insulin-regulatable glucose transporter (IRGT)3,10, is primarily expressed in muscle and fat, tissues that exhibit insulin-dependent glucose transport. Insulin promotes glucose transport in these tissues by stimulating movement of the glucose transporter from an intracellular location to the plasma membrane3,10–14. Recent studies15, however, suggest that an additional effect of insulin in these tissues may be the facilitation of glucose transport, presumably across capillary endothelium. This hypothesis is based on the localization of the IRGT in endothelial cells specific to muscle and adipose tissue. We report here, however, on morphological and biochemical studies using several different IRGT-specific antibodies in which we could not reproduce these results.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Morgan, H. E. & Whitfield, C. F. Curr. Top. Membranes Transp. 4, 255–303 (1973).
Elbrink, J. & Bihler, I. Science 188, 1177–1184 (1975).
James, D. E., Strube, M. & Mueckler, M. Nature 338, 83–87 (1989).
Birnbaum, M. J. Cell 57, 305–315 (1989).
Charron, M. J., Brosius, F. C., Alper, S. L. & Lodish, H. F. Proc. natn. Acad. Sci. U.S.A. 86, 2535–2539 (1989).
Kaestner, K. H. et al. Proc. natn. Acad. Sci. U.S.A. 86, 3150–3154 (1989).
Fukumoto, H. et al. J. biol. Chem. 264, 7776–7779 (1989).
Mueckler, M. et al. Science 229, 941–945 (1985).
Thorens, B., Sarkar, H. K., Kaback, H. R. & Lodish, H. F. Cell 55, 281–290 (1988).
James, D. E., Brown, R., Navarro, J. & Pilch, P. F. Nature 333, 183–185 (1988).
Suzuki, K. & Kono, T. Proc. natn. Acad. Sci. U.S.A. 77, 2542–2545 (1980).
Cushman, S. W. & Wardzala, L. J. J. biol. Chem. 255, 2758–2762 (1980).
Wardzala, L. J. & Jeanrenaud, B. J. biol. Chem. 256, 7090–7093 (1981).
Watanabe, T., Smith, M. M., Robinson, F. W. & Kono, T. J. biol. Chem. 259, 13117–13133 (1984).
Vilaro, S., Palacin, M., Pilch, P. F., Testar, X. & Zorzano, A. Nature 342, 789–800 (1989).
Maruyama, I., Bell, C. E. & Majerus, P. W. J. Cell Biol. 101, 363–371 (1985).
Cheung, J. Y., Conover, C., Regen, D. M., Whitfield, C. F. & Morgan, H. E. Am. J. Physiol. 234, E70–78 (1978).
Kuikka, J., Levin, M. & Bassingthwaighte, J. B. Am. J. Physiol. 250, H29–42 (1986).
Slot, J. W., Geuze, H. J. & Weerkamp, A. H. J. meth. Microbiol. 20, 211–236 (1988).
Kern, P. A., Knedler, A. & Eckel, R. H. J. clin. Invest. 71, 1822–1829 (1983).
Kumeda, T., Dittman, W. A. & Majerus, P. W. Blood 71, 728–733 (1987).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Slot, J., Moxley, R., Geuze, H. et al. No evidence for expression of the Insulin-regulatable glucose transporter in endothelial cells. Nature 346, 369–371 (1990). https://doi.org/10.1038/346369a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/346369a0
This article is cited by
-
Immunohistochemical localization of Na+-dependent glucose transporter in rat jejunum
Cell & Tissue Research (1992)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
