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A new human immunoglobulin VH family preferentially rearranged in immature B-cell tumours

Nature volume 331, pages 446–449 (1988)Cite this article

Abstract

The prevalent forms of adult and childhood B-cell neoplasia are chronic lymphocytic (CLL) and acute lymphocytic (ALL) leukaemia, and are typified by a nearly monoclonal accumulation of cells expressing a single heavy (H) and light (L) chain variable (V) region. V gene selection could be random, or quite biased if the disease or the developmental status of the transformed cell somehow influenced DNA rearrangement. We have cloned and sequenced three germ-line VH gene segments that constitute a new human VH family (subgroup V) linked within 160 kilobase pairs of the DH-JH complex. One VH(V) member is rearranged in about 30% of patients with CLL and ALL, but not in IgM-expressing B-cell lines from peripheral blood. In some tumours, we detect a truncated ( VH(V) RNA devoid of constant regions that originates from unrearranged VH(V) genes. In other tumours and in resting splenocytes, we detect large amounts of normally sized VH(V)-associated mRNA, although stimulation by mitogen of splenic B cells results in loss of VH(V)-hybridizing RNA. These features suggest that biased rearrangement of subgroup V may be under developmental selection.

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References

  1. Riblet, R. & Brodeur, P. H. in Genetics and Molecular Immunology (eds. Herzenberg, L. A., Blackwell, C. & Herzenberg, L. A.) 89.1–89.6 (Blackwell, Oxford, 1986).

    Google Scholar 

  2. Yancopoulos, G. D. et al. Nature 311, 727–733 (1984).

    Article  ADS  CAS  Google Scholar 

  3. Kabat, E. A. et al. Sequences of Proteins of Immunological Interest Vol. 4 (U.S. Dept. of Health and Human Services, 1987).

    Google Scholar 

  4. Kodaira, M. et al. J. molec Biol 190, 529–541 (1986).

    Article  CAS  Google Scholar 

  5. Rechavi, G. et al. Proc. natn. Acad. Sci. U.S.A. 80, 855–859 (1983).

    Article  ADS  CAS  Google Scholar 

  6. Rechavi, G. et al. Proc. natn. Acad. Sci. U.S.A. 79, 4405–4409 (1982).

    Article  ADS  CAS  Google Scholar 

  7. Matthyssens, G. & Rabbitts, T. H. Proc. natn. Acad. Sci. U.S.A. 77, 6561–6565 (1980).

    Article  ADS  CAS  Google Scholar 

  8. Lee, K. H., Matsuda, F., Kinashi, T., Kodaira, M. & Honjo, T. J. Molec. Biol. 195, 761–768 (1987).

    Article  CAS  Google Scholar 

  9. Blattner, W. et al. Ann intern. Med. 84, 554–557 (1976).

    Article  CAS  Google Scholar 

  10. Shen, A., Humphries, C., Tucker, P. W. & Blattner, F. R. Proc. natn. Acad. Sci. U.S.A. 84, 8563–8567 (1987).

    Article  ADS  CAS  Google Scholar 

  11. Parslow, T. G., Blair, D. L., Murphy, N. J. & Granner, D. K. Proc. natn. Acad. Sci. U.S.A. 81, 2650–2654 (1984).

    Article  ADS  CAS  Google Scholar 

  12. Rathbun, G. A., Capra, J. D. & Tucker, P. W. EMBO J. 10, 2931–2937 (1987).

    Article  Google Scholar 

  13. Yancopuolos, G. D. & Alt, F. W. Cell 40, 271–281 (1985).

    Article  Google Scholar 

  14. Wu, G. E. & Paige, C. J. EMBO J. 5, 3475–3481 (1986).

    Article  CAS  Google Scholar 

  15. Riley, S. C., Connors, S. J., Klinman, N. R. & Ogata, R. T. Proc. natn. Acad. Sci. U.S.A. 83, 2589–2592 (1986).

    Article  ADS  CAS  Google Scholar 

  16. Perlmutter, R. M., Kearney, J. F., Chang, S. P. & Hood, L. G. Science 227, 1597–1601 (1985).

    Article  ADS  CAS  Google Scholar 

  17. Infante, P., Wagoner, J. & Rinskey, R. Lancet ii, 76–78 (1977).

    Article  Google Scholar 

  18. Blair, A., Decoufle, P. & Grauman, D. Am. J. Public Health 69, 508–511 (1979).

    Article  CAS  Google Scholar 

  19. Mann, D. L. et al. Science 236, 1103–1106 (1987).

    Article  ADS  CAS  Google Scholar 

  20. Boumsell, L. H. et al. J. exp. Med. 152, 229–239 (1980).

    Article  CAS  Google Scholar 

  21. Casali, P., Inghirami, G., Nakamura, M., Davies, T. F. & Notkins, A. L. Science 234, 476–480 (1986).

    Article  ADS  CAS  Google Scholar 

  22. Hayakawa, K., Hardy, R. R., Parks, D. R. & Herzenberg, L. A. J. exp. Med. 157, 202–207 (1983).

    Article  CAS  Google Scholar 

  23. Manheimer-Lory, A., Momestier, M., Bellan, B., Alt, F. & Bona, C. Proc. natn. Acad. Sci. U.S.A. 83, 8293–8297 (1986).

    Article  ADS  CAS  Google Scholar 

  24. Kunkel, H. G., Agnello, V., Joslin, F. G., Winchester, R. J. & Capra, J. D. J. exp. Med. 137, 331–342 (1979).

    Article  Google Scholar 

  25. Kipps, T. J. et al. Proc. natn. Acad. Sci. U.S.A. 84, 2916–2920 (1986).

    Article  ADS  Google Scholar 

  26. Fu, S. M., Chiorazzi, N. & Kunkel, H. G. Immunol. Rev. 48, 23–35 (1979).

    Article  CAS  Google Scholar 

  27. Hayakawa, K. et al. Proc. natn. Acad. Sci. U.S.A. 81, 2494–2498 (1984).

    Article  ADS  CAS  Google Scholar 

  28. Antin, J. H., Emerson, S. G., Martin, P., Gadol, N. & Ault, K. A. J. Immun. 136, 505–510 (1986).

    CAS  PubMed  Google Scholar 

  29. Blin, N. & Stafford, D. W. Nucleic Acids Res. 3, 2303–2308 (1976).

    Article  CAS  Google Scholar 

  30. Liu, C.-P., Tucker, P. W., Mushinski, J. F. & Blattner, F. R. Science 209, 1348–1352 (1980).

    Article  ADS  CAS  Google Scholar 

  31. Loenen, W. & Blattner, F. R. Gene 26, 171–179 (1983).

    Article  CAS  Google Scholar 

  32. Tucker, P. W. in Genetics and Molecular Immunology (eds Herzenberg, L. A., Blackwell, C. & Herzenberg, L. A.) 86.1–86.16 (Blackwell, Oxford, 1986).

    Google Scholar 

  33. Viera, J. & Messing J. Gene 19, 771–780 (1982).

    Google Scholar 

  34. Maxam, A. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    Article  CAS  Google Scholar 

  35. Bencini, D. A., O'Donovan, G. A. & Wild, J. R. Biotechniques 2, 4–12 (1984).

    Google Scholar 

  36. Rubin, C. M. & Schmidt, C. W. Nucleic Acids Res. 8, 4613–4622 (1980).

    Article  CAS  Google Scholar 

  37. Hururitz, R. et al. Int. J. Cancer. 23, 174–180 (1979).

    Article  Google Scholar 

  38. Hieter, P. A., Hollis, G. F., Korsmeyer, S. J., Waldmann, T. A. & Leder, P. Nature 24, 536–540 (1981).

    Article  ADS  Google Scholar 

  39. Van der Ploeg, L. H., Schwartz, D. C., Cantor, C. R. & Borst, P. Cell 37, 77–84 (1984).

    Article  CAS  Google Scholar 

  40. Carle, G. F. & Olsen, M. V. Nucleic Acids Res. 12, 5647–5664 (1984).

    Article  CAS  Google Scholar 

  41. Ravetch, J. et al. Cell 27, 583–591 (1981).

    Article  CAS  Google Scholar 

  42. Chirgwin, J. M., Przybyla, A. S., MacDonald, R. J. & Rutter, W. J. Biochemistry 18, 5294–5304 (1979).

    Article  CAS  Google Scholar 

  43. Tutt, M. et al. J. Immun. 137, 2998–3001 (1986).

    CAS  PubMed  Google Scholar 

  44. Rabbitts, T. H., Forster, A. & Milstein, C. P. Nucleic Acids Res. 9, 4509–4525 (1981).

    Article  CAS  Google Scholar 

  45. Schroeder, H. W., Hilson, J. L. & Pearlmutter, R. M. Science 238, 791–793 (1987).

    Article  ADS  CAS  Google Scholar 

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Author notes

  1. A. Shen and F. R. Blattner: Department of Genetics, University of Wisconsin, Madison, Wisconsin 53706, USA

Authors and Affiliations

  1. Department of Microbiology, University of Texas Health Science Center, Dallas, Texas, 75235, USA

    C. G. Humphries, A. Shen, W. A. Kuziel, J. D. Capra, F. R. Blattner & P. W. Tucker

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  1. C. G. Humphries
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  2. A. Shen
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  3. W. A. Kuziel
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  4. J. D. Capra
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  5. F. R. Blattner
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  6. P. W. Tucker
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Humphries, C., Shen, A., Kuziel, W. et al. A new human immunoglobulin VH family preferentially rearranged in immature B-cell tumours. Nature 331, 446–449 (1988). https://doi.org/10.1038/331446a0

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