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Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes

Nature volume 308, pages 421–424 (1984)Cite this article

Abstract

Sodium channels and receptors to serotonin and kainate were ‘transplanted’ from human brain into frog oocytes, by isolating messenger RNA from a fetal brain, and injecting it into Xenopus laevis oocytes. The mRNA was translated by the oocyte and induced the appearance of functional receptors and channels in its membrane. This approach renders drug- and voltage-operated channels of the human brain more amenable to detailed study.

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Authors and Affiliations

  1. Department of Biophysics, University College London, Gower Street, London, WC1E 6BT, UK

    C. B. Gundersen, R. Miledi & I. Parker

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  1. C. B. Gundersen
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  2. R. Miledi
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  3. I. Parker
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Gundersen, C., Miledi, R. & Parker, I. Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes. Nature 308, 421–424 (1984). https://doi.org/10.1038/308421a0

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