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Small-angle X-ray scattering from myosin heads in relaxed and rigor frog skeletal muscles

Nature volume 303, pages 146–152 (1983)Cite this article

Abstract

Low-angle X-ray diffraction patterns from relaxed and non-overlap rigor muscles show a central region of diffuse scattering (disk) which is circularly symmetrical, behaves as solution scattering and comes predominantly from myosin heads. In full-overlap rigor the disk is compressed in the diagonal direction, indicating that the myosin heads have a bent shape and a preferred orientation consistent with a 45° angle of attachment to actin.

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References

  1. Hanson, J. & Huxley, H. E. Nature 172, 530–532 (1953).

    Article  ADS  CAS  Google Scholar 

  2. Huxley, A. F. & Niedergerke, R. Nature 173, 971–973 (1954).

    Article  ADS  CAS  Google Scholar 

  3. Hanson, J. & Huxley, H. E. Symp. Soc. exp. Biol. 9, 228–264 (1955).

    Google Scholar 

  4. Huxley, H. E. J. Biophys. Biochem. Cytol. 3, 631–648 (1957).

    Article  CAS  Google Scholar 

  5. Huxley, H. E. & Hanson, J. Proc. Stockholm Conf. Electron Microscopy, 202–204 (Academic, New York, 1957).

    Google Scholar 

  6. Lowy, J. & Hanson, J. Physiol. Rev. 42, 34–47 (1962).

    Google Scholar 

  7. Lowey, S. in Fibrous Proteins: Scientific, Industrial & Medical Aspects (eds Parry, D. A. D. & Creamer, L. K.) 1–25 (Academic, New York, 1979).

    Google Scholar 

  8. Huxley, H. E. & Brown, W. J. molec. Biol. 30, 384–434 (1967).

    Article  Google Scholar 

  9. Squire, J. M. A. Rev. Biophys. Bioengng 4, 137–163 (1975).

    Article  CAS  Google Scholar 

  10. Luther, P. K. & Squire, J. M. J. molec. Biol. 141, 409–439 (1980).

    Article  CAS  Google Scholar 

  11. Huxley, H. E., Faruqi, A. R., Bordas, J., Koch, M. H. J. & Milch, J. R. Nature 284, 140–143 (1980).

    Article  ADS  CAS  Google Scholar 

  12. Huxley, H. E. et al. Proc. natn. Acad. Sci. U.S.A. 78, 2297–2301 (1981).

    Article  ADS  CAS  Google Scholar 

  13. Huxley, H. E., Faruqi, A. R., Kress, M., Bordas, J. & Koch, M. H. J. J. molec. Biol. 158, 637–684 (1982).

    Article  CAS  Google Scholar 

  14. Vibert, P. J., Haselgrove, J. C., Lowy, J. & Poulsen, F. R. J. molec. Biol. 71, 757–767 (1972).

    Article  CAS  Google Scholar 

  15. Haselgrove, J. C. J. molec. Biol. 92, 113–143 (1975).

    Article  CAS  Google Scholar 

  16. Mendelson, R. A. & Kretzschmar, K. M. Biochemistry 19, 4103–4108 (1980).

    Article  CAS  Google Scholar 

  17. Mendelson, R. A. Nature 298, 665–667 (1982).

    Article  ADS  CAS  Google Scholar 

  18. Reedy, M. K., Holmes, K. C. & Tregear, R. T. Nature 207, 1276–1280 (1965).

    Article  ADS  CAS  Google Scholar 

  19. Thomas, D. D., Ishiwata, S., Seidel, J. C. & Gergely, J. Biophys. J. 32, 873–889 (1980).

    Article  CAS  Google Scholar 

  20. Borejdo, J., Assulin, O., Ando, T. & Putnam, S. J. molec. Biol. 158, 391–414 (1982).

    Article  CAS  Google Scholar 

  21. Thomas, D. D. & Cooke, R. Biophys. J. 32, 891–906 (1980).

    Article  ADS  CAS  Google Scholar 

  22. Scopes, R. K. in Physiology and Biochemistry of Muscle as a Food (eds Briskey, E. J., Cassens, R. G. & Trautmann, J. C.) 471–492 (University of Wisconsin Press, Madison, 1966).

    Google Scholar 

  23. Davson, H. & Eggleton, M. G. in Principles of Human Physiology, 799 (Lea & Febiger, Philadelphia, 1962).

    Google Scholar 

  24. Kendrick-Jones, J. et al. J. Muscle Res. Cell Motility 3, 477 (1982).

    Google Scholar 

  25. Sutoh, K., Sutoh, K., Karr, T. & Harrington, W. F. J. molec. Biol. 126, 1–22 (1978).

    Article  CAS  Google Scholar 

  26. Holmes, K. C., Tregear, R. T. & Barrington Leigh, J. Proc. R. Soc. 207, 13–33 (1980).

    ADS  Google Scholar 

  27. Lovell, S. J., Knight, P. J. & Harrington, W. F. Nature 293, 664–666 (1981).

    Article  ADS  CAS  Google Scholar 

  28. Yagi, N., O'Brien, E. J. & Matsubara, I. Biophys. J. 33, 121–138 (1981).

    Article  CAS  Google Scholar 

  29. Matsubara, I. & Millman, B. M. J. molec. Biol. 82, 527–536 (1974).

    Article  CAS  Google Scholar 

  30. Lowy, J. & Poulsen, F. R. Nature 299, 308–312 (1982).

    Article  ADS  CAS  Google Scholar 

  31. Jewell, B. R. & Wilkie, D. R. J. Physiol., Lond. 152, 30–47 (1960).

    Article  CAS  Google Scholar 

  32. Guinier, A. X-ray Diffraction in Crystals, Imperfect Crystals, and Amorphous Bodies, 185–294, 319–350 (Freeman, San Francisco, 1963).

    Google Scholar 

  33. Kretzschmar, K. M., Mendelson, R. A. & Morales, M. F. Biochemistry 17, 2314–2318 (1978).

    Article  CAS  Google Scholar 

  34. Glatter, O. in Small Angle X-ray Scattering (eds Glatter, O. & Kratky, O) 119–165 (Academic, London, 1982).

    Google Scholar 

  35. Porod, G. Kolloidzeitschrift 124, 51–57 (1951), 125, 108–122 (1952).

    Google Scholar 

  36. Debye, P. Ann. Phys. 43, 49–95 (1914).

    CAS  Google Scholar 

  37. Waller, I. Z. Phys. 17, 398–408 (1923).

    Article  ADS  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Oxford Research Unit, The Open University, Foxcombe Hall, Berkeley Road, Boars Hill, Oxford, OX1 5HR, UK

    F. R. Poulsen & J. Lowy

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  1. F. R. Poulsen
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  2. J. Lowy
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Poulsen, F., Lowy, J. Small-angle X-ray scattering from myosin heads in relaxed and rigor frog skeletal muscles. Nature 303, 146–152 (1983). https://doi.org/10.1038/303146a0

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