Abstract
A library of 50,000 recombinants representative of the human X chromosome has been constructed. Human X chromosomes were physically separated using a fluorescence-activated cell sorter. The DNA was purified from the chromosomes, digested to completion with the restriction enzyme EcoRI and cloned into the phage λgtWES.λB. The X-derived nature of the recombinants was confirmed by hybridization to rodent/human cell line DNA containing only the human X chromosome. Such libraries will be particularly useful for the investigation of genetic diseases such as Duchenne muscular dystrophy, where the basic defect has not been elucidated, and of neoplasia, where several specific chromosomal anomalies, particularly for the leukaemias, have been identified.
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References
Gusella, J. F. et al. Proc. natn. Acad. Sci. U.S.A. 77, 2829–2833 (1980).
Olsen, A. S., McBride, O. W. & Otey, M. C. Biochemistry 19, 2419–2428 (1980).
Schmeckpeper, B. J., Smith, K. D., Dorman, B. P., Ruddle, F. H. & Talbot, C. C. Proc. natn. Acad. Sci. U.S.A. 76, 6525–6528.
Ohno, S. Cold Spring Harb. Symp. quant. Biol. 38, 155–164 (1973).
Kunkel, L. M., Smith, K. D. & Boyer, S. H. Science 191, 1189–1190 (1976).
Kunkel, L. M. et al. Proc. natn. Acad. Sci. U.S.A. 74, 1245–1249 (1977).
Wolf, S. F., Mareni, C. E. & Migeon, B. R. Cell 21, 95–102 (1980).
Sillar, R. & Young, B. D. J. Histochem. Cytochem. 29, 74–78 (1981).
Carrano, A. V., Gray, J. W., Langlois, R. S., Burkhart-Schultz, K. J. & Van Dilla, M. A. Proc. natn. Acad. Sci. U.S.A. 76, 1382–1384 (1979).
Young, B. D., Ferguson-Smith, M. A., Sillar, R. & Boyd, E. Proc. natn. Acad. Sci. U.S.A. (submitted).
Leder, P., Tiemeier, D. & Enquist, L. Science 196, 175–177 (1977).
Enquist, L. & Sternberg, N. Meth. Enzym. 68, 281–286 (1979).
Blattner, F. R. et al. Science 202, 1279–1283 (1978).
Schmid, C. W. & Dehninger, P. L. Cell 6, 345–358 (1975).
Crampton, J. M., Davies, K. E. & Knapp, T. C. Nucleic Acids Res. (in the press).
Goodfellow, P., Banting, G., Levy, R., Povey, S. & McMichael, A. Somat. Cell Genet. 6, 777–787 (1980).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Clarke, L. & Carbon, J. Cell 9, 91–99 (1976).
Loenen, W. A. M. & Brammar, W. J. Gene 20, 249–259 (1980).
Jeffreys, A. J. & Flavell, R. A. Cell 12, 429–439 (1977).
Jeffreys, A. J. & Flavell, R. A. Cell 12, 1097–1108 (1977).
Rigby, P. W. J., Dieckmann, M., Rhodes, C. & Berg, P. J. molec. Biol. 113, 237–251 (1976).
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Davies, K., Young, B., Elles, R. et al. Cloning of a representative genomic library of the human X chromosome after sorting by flow cytometry. Nature 293, 374–376 (1981). https://doi.org/10.1038/293374a0
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DOI: https://doi.org/10.1038/293374a0
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