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Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genes

Abstract

A putative diversity segment of immunoglobulin heavy-chain genes (D segment) has been identified 700 base pairs 5′ to JH1 DNA on the germ-line genome of the mouse. This 10-base pair D segment is flanked by two sets of sequences related to

which are possible recognition sites for a recombinase. The spacer separating the heptamer and the nonamer is 12 base pairs long on both sides of the D segment. As the spacer separating the two signal sequences in VH DNAs and JH DNAs is 23 ± 1 base pairs long, the two recombinations required for creation of a complete immunoglobulin VHgene, a VH–D joining and a D–JH joining, follow a 12/23-base pair spacer rule. Allelic exclusion is discussed with respect to D segments.

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Sakano, H., Kurosawa, Y., Weigert, M. et al. Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genes. Nature 290, 562–565 (1981). https://doi.org/10.1038/290562a0

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