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Analysis of regulation of Klebsiella pneumoniae nitrogen fixation (nif) gene cluster with gene fusions

Nature volume 286, pages 128–132 (1980)Cite this article

Abstract

Gene fusions in which the lac genes are under the control of each promoter in the Klebsiella pneumoniae nitrogen fixation (nif) gene cluster have been constructed. These fusions have been used to examine positive control of the cluster and the response of individual genes to repression by ammonia and oxygen. De-repression of nif transcriptional units is coordinate and molybdate is required for maximal expression of the structural gene operon, which is autogenously regulated.

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References

  1. Eady, R. R. & Smith, B. E. in A Treatise on Dinitrogen Fixation, Sections I, II (eds Hardy, R. W., Bottemly, F. & Burns, R. C.) 399–490 (Wiley, New York, 1979).

    Google Scholar 

  2. Shah, V. K. & Brill, W. J. Proc. natn. Acad. Sci. U.S.A. 74, 3249–3253 (1977).

    Article  ADS  CAS  Google Scholar 

  3. Eady, R. R. & Postgate, J. R. Nature 249, 805–810 (1974).

    Article  ADS  CAS  Google Scholar 

  4. Merrick, M., Filser, M., Kennedy, C. & Dixon, R. Molec. gen. Genet. 165, 103–111 (1978).

    Article  CAS  Google Scholar 

  5. Elmerich, C., Houmard, J., Sibold, L., Manheimer, I. & Charpin, N. Molec. gen. Genet. 165, 181–189 (1978).

    Article  CAS  Google Scholar 

  6. MacNeil, T., MacNeil, D., Roberts, G. P., Supiano, M. A. & Brill, W. J. J. Bact. 136, 253–266 (1978).

    CAS  PubMed  Google Scholar 

  7. Merrick, M. et al. J. gen. Microbiol. 117, 509–520 (1980).

    CAS  PubMed  Google Scholar 

  8. Roberts, G. P., MacNeil, T., MacNeil, D. & Brill, W. J. J. Bact. 136, 267–279 (1978).

    CAS  PubMed  Google Scholar 

  9. Hill, S. & Kavanagh, E. J. Bact. 141, 470–475 (1980).

    CAS  PubMed  Google Scholar 

  10. Dixon, R., Kennedy, C., Kondorosi, A., Krishnapillai, V. & Merrick, M. Mol. gen. Genet. 157, 189–198 (1977).

    Article  CAS  Google Scholar 

  11. Eady, R. R., Issack, R., Kennedy, C., Postgate, J. R. & Ratcliffe, H. D. J. gen. Microbiol. 104, 277–285 (1978).

    Article  CAS  Google Scholar 

  12. Brill, W. J., Steiner, A. L. & Shah, V. K. J. Bact. 118, 986–989 (1974).

    CAS  PubMed  Google Scholar 

  13. Tyler, B. A. Rev. Biochem. 47, 1127–1162 (1978).

    Article  CAS  Google Scholar 

  14. Casadaban, M. & Cohen, S. N. Proc. natn. Acad. Sci. U.S.A. 76, 4530–4533 (1979).

    Article  ADS  CAS  Google Scholar 

  15. Janssen, K. A., Riedel, G. E., Ausubel, F. M. & Cannon, F. C. in Proc. 3rd int. Symp. Nitrogen Fixation, Vol. 1 (eds Orme-Johnson, W. H. & Newton, W.) 85–93 (University Park Press, Baltimore, 1980).

    Google Scholar 

  16. Pahel, G. & Tyler, B. Proc. natn. Acad. Sci. U.S.A. 76, 4544–4548 (1979).

    Article  ADS  CAS  Google Scholar 

  17. Kustu, S., Burton, D., Garcia, E., McCarter, L. & McFarland, N. Proc. natn. Acad. Sci. U.S.A. 76, 4576–4580 (1979).

    Article  ADS  CAS  Google Scholar 

  18. Ausubel, F. M., Reidel, G., Cannon, F., Peskin, A. & Margolskee, R. in Genetic-Engineering for Nitrogen Fixation (ed. Hollaender. A.) 111–126 (Plenum, New York, 1977).

    Book  Google Scholar 

  19. Kennedy, C. & Postgate, J. R. J. gen. Microbiol. 98, 551–557 (1977).

    Article  CAS  Google Scholar 

  20. Cannon, F. C., Dixon, R. A., Postgate, J. R. & Primrose, S. B. J. gen. Microbiol. 80, 227–239 (1974).

    Article  CAS  Google Scholar 

  21. Kelly, M. & Lang, G. Biochim. biophys. Acta 223, 86–104 (1970).

    Article  CAS  Google Scholar 

  22. Miller, J. H. in Experiments in Molecular Genetics, 162–172 (Cold Spring Harbor Laboratory, New York, 1972).

    Google Scholar 

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Author information

Author notes

  1. Maurizio Iaccarino: CNR Istituto Internazionale di Genetica et Biofisica, Via Gugliemo Marconi 10, 80125 Naples, Italy

Authors and Affiliations

  1. ARC Unit of Nitrogen Fixation, University of Sussex, Brighton, BN1 9RQ, UK

    Ray Dixon, Robert R. Eady, Guadalupe Espin, Susan Hill, Maurizio Iaccarino, Daniel Kahn & Mike Merrick

Authors
  1. Ray Dixon
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  2. Robert R. Eady
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  3. Guadalupe Espin
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  4. Susan Hill
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  5. Maurizio Iaccarino
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  6. Daniel Kahn
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  7. Mike Merrick
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Dixon, R., Eady, R., Espin, G. et al. Analysis of regulation of Klebsiella pneumoniae nitrogen fixation (nif) gene cluster with gene fusions. Nature 286, 128–132 (1980). https://doi.org/10.1038/286128a0

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