Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Order and intracellular location of the events involved in the maturation of a spliced tRNA

Nature volume 284, pages 143–148 (1980)Cite this article

Abstract

Microinjected frog oocytes were used to analyse the RNA processing steps which lead to the appearance of a mature cytoplasmic tRNAtyr molecule. The results show that removal of the intervening sequence from within a yeast tRNAtyr precursor, excision of extra 3′ and 5′ nucleotides, addition of a 3′-terminal CCA and modification of at least seven ribonucleotides all occur in the nucleus before the tRNAtyr is transported to the cytoplasm. Moreover, we find that the ribonucleotide modifications occur in a strict order which precisely correlates with the size alterations of the tRNAtyr precursor.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Burdon, R. H. Prog. Nucleic Acid Res. 11, 33–79 (1971).

    Article  CAS  Google Scholar 

  2. Robertson, H. D. & Dickson, E. Brookhaven Symp. Biol. 26, 240–266 (1974).

    CAS  Google Scholar 

  3. Darnell, J. E., Jelinek, W. R. & Molloy, G. R. Science 181, 1215–1221 (1973).

    Article  ADS  CAS  Google Scholar 

  4. Smith, J. D. Prog. Nucleic Acid Res. 16, 25–73 (1976).

    Article  CAS  Google Scholar 

  5. Goodman, H., Olson, M. V. & Hall, B. D. Proc. natn. Acad. Sci. U.S.A. 74, 5453–5457 (1977).

    Article  ADS  CAS  Google Scholar 

  6. O'Farrell, P., Cordell, B., Valenzuela, P., Rutter, W. J. & Goodman, H. M. Nature 274, 438–445 (1978).

    Article  ADS  CAS  Google Scholar 

  7. Valenzuela, P., Venegas, A., Weinberg, F., Bishop, R. & Rutter, W. J. Proc. natn. Acad. Sci. U.S.A. 75, 190–194 (1978).

    Article  ADS  CAS  Google Scholar 

  8. Knapp, G., Beckmann, J. S., Johnson, P. F., Fuhrman, S. A. & Abelson, J. Cell 14, 221–236 (1978).

    Article  CAS  Google Scholar 

  9. Etcheverry, T., Colby, D. & Guthrie, C. Cell 18, 11–26 (1979).

    Article  CAS  Google Scholar 

  10. Gilmore, R., Stewart, J. & Sherman, F. J. molec. Biol. 61, 157–173 (1971).

    Article  CAS  Google Scholar 

  11. Olson, M. et al. Nature 267, 639–641 (1977).

    Article  ADS  CAS  Google Scholar 

  12. De Robertis, E. M. & Olson, M. V. Nature 278, 137–143 (1979).

    Article  ADS  CAS  Google Scholar 

  13. Gurdon, J. B. Proc. R. Soc. B198, 211–247 (1977).

    ADS  CAS  Google Scholar 

  14. Mertz, J. E. & Gurdon, J. B. Proc. natn. Acad. Sci. U.S.A. 74, 1502–1506 (1977).

    Article  ADS  CAS  Google Scholar 

  15. Brown, D. D. & Gurdon, J. B. Proc. natn. Acad. Sci. U.S.A. 74, 2064–2068 (1977).

    Article  ADS  CAS  Google Scholar 

  16. Kressmann, A., Clarkson, S. G., Telford, J. & Birnstiel, M. L. Cold Spring Harb. Symp. quant. Biol. 42, 1077–1082 (1978).

    Article  CAS  Google Scholar 

  17. Kressmann, A., Clarkson, S. G., Pirotta, V. & Birnstiel, M. L. Proc. nat. Acad. Sci. U.S.A. 75, 1176–1180 (1978).

    Article  ADS  CAS  Google Scholar 

  18. Cortese, R., Melton, D., Tranquilla, T. & Smith, J. D. Nucleic Acids Res. 5, 4593–4611 (1978).

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Birkenmeier, E. H., Brown, D. D. & Jordan, E. Cell 15, 1077–1086 (1978).

    Article  CAS  Google Scholar 

  20. Schmidt, O., Mao, J., Silverman, S., Hovemann, B. & Söll, D. Proc. natn. Acad. Sci. U.S.A. 75, 4819–4823 (1978).

    Article  ADS  CAS  Google Scholar 

  21. Ogden, R. C. et al. Cell 17, 399–406 (1979).

    Article  CAS  Google Scholar 

  22. Hagenbüchle, O., Larson, D., Hall, G. & Sprague, K. Cell 18, 1217–1229 (1979).

    Article  Google Scholar 

  23. De Robertis, E. M. & Mertz, J. E. Cell 12, 175–182 (1977).

    Article  CAS  Google Scholar 

  24. Rungger, D. & Türler, H. Proc. natn. Acad. Sci. U.S.A. 75, 6073–6077 (1978).

    Article  ADS  CAS  Google Scholar 

  25. Melton, D. & Cortese, R. Cell 18, 1165–1172 (1979).

    Article  CAS  Google Scholar 

  26. Deutscher, M. Prog. Nucleic Acid Biochem. 13, 51–92 (1973).

    Article  CAS  Google Scholar 

  27. Solari, A., Gatica, M. & Allende, J. Nucleic Acids Res. 4, 1873–1880 (1977).

    Article  CAS  Google Scholar 

  28. Daniel, V. & Littauer, U. J. molec. Biol. 11, 692–705 (1965).

    Article  CAS  Google Scholar 

  29. Brownlee, H. Determination of Sequences in RNA (North-Holland, New York, 1972).

    Book  Google Scholar 

  30. Burdon, R., Martin, B. & Lal, B. J. molec. Biol. 28, 357–371 (1967).

    Article  CAS  Google Scholar 

  31. Muramatsu, M. & Fujisawa, F. Biochim. biophys. Acta 157, 476–492 (1968).

    Article  CAS  Google Scholar 

  32. Moshowitz, D. J. molec. Biol. 50, 143–151 (1970).

    Article  Google Scholar 

  33. Koski, R., Bothwell, A. & Altaian, S. Cell 9, 101–116 (1976).

    Article  CAS  Google Scholar 

  34. Lönn, U. J. molec. Biol. 112, 661–666 (1977).

    Article  Google Scholar 

  35. Schmidt, F., Seidman, J. & Bock, R. J. biol. Chem. 251, 2440–2445 (1976).

    CAS  PubMed  Google Scholar 

  36. Seidman, J. & McClain, W. Proc. natn. Acad. Sci. U.S.A. 72, 1491–1495 (1975).

    Article  ADS  CAS  Google Scholar 

  37. Altaian, S., Bothwell, A. L. M. & Stark, B. C. Brookhaven Symp. Biol. 26, 12–25 (1974).

    Google Scholar 

  38. Schedl, P., Primakoff, P. & Roberts, S. Brookhaven Symp. Biol. 26, 53–76 (1974).

    CAS  Google Scholar 

  39. Garber, R. L. & Altaian, S. Cell 17, 389–397 (1979).

    Article  CAS  Google Scholar 

  40. Guthrie, C. et al. Brookhaven Symp. Biol. 26, 106–123 (1974).

    CAS  Google Scholar 

  41. Schaefer, K. P., Altaian, S. & Söll, D. Proc. natn. Acad. Sci. U.S.A. 70, 3626–3630 (1973).

    Article  ADS  CAS  Google Scholar 

  42. Merriam, R. W. & Hill, R. J. J. Cell Biol. 69, 659–668 (1978).

    Article  Google Scholar 

  43. Ford, C. C. & Gurdon, J. B. J. Embryol. exp. Morph. 37, 203–209 (1977).

    CAS  PubMed  Google Scholar 

  44. Barrell, B. G. Prog. Nucleic Acid Res. 2, 751–779 (1971).

    CAS  Google Scholar 

  45. Harada, F. & Nishimura, S. Biochemistry 11, 301–308 (1972).

    Article  CAS  Google Scholar 

  46. Probst, E., Kressmann, A. & Birnstiel, M. L. J. molec. Biol. 135, 709–732 (1979).

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. R. Cortese

    Present address: European Molecular Biology Laboratory, 6900, Heidelberg, FRG

Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

    D. A. Melton, E. M. De Robertis & R. Cortese

Authors
  1. D. A. Melton
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. M. De Robertis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Cortese
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Melton, D., De Robertis, E. & Cortese, R. Order and intracellular location of the events involved in the maturation of a spliced tRNA. Nature 284, 143–148 (1980). https://doi.org/10.1038/284143a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/284143a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing