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Transformation of yeast by a replicating hybrid plasmid

Nature volume 275, pages 104–109 (1978)Cite this article

Abstract

Chimaeric plasmids have been constructed containing a yeast plasmid and fragments of yeast nuclear DNA linked to pMB9, a derivative of the ColEl plasmid from E. coli. Two plasmids were isolated which complement leuB mutations in E. coli. These plasmids have been used to develop a method for transforming a leu2 strain of S. cerevisiae to Leu+ with high frequency. The yeast transformants contained multiple plasmid copies which were recovered by transformation in E. coli. The yeast plasmid sequence recombined intramolecularly during propagation in yeast.

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Authors and Affiliations

  1. Department of Molecular Biology, University of Edinburgh, Edinburgh, UK

    Jean D. Beggs

  2. Department of Cytogenetics, Plant Breeding Institute, Trumpington, Cambridge, UK

    Jean D. Beggs

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  1. Jean D. Beggs
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Beggs, J. Transformation of yeast by a replicating hybrid plasmid. Nature 275, 104–109 (1978). https://doi.org/10.1038/275104a0

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