Abstract
IT is known1–4 that the AAF-addition products obtained in the reaction of the ultimate carcinogen, N-acetoxy-N-2-acetylamino-fluorene (AAAF) with DNA in vitro are essentially the same as those isolated from liver DNA of rats fed with N-2-acetyl-aminofluorene (AAF). Thus, the modifications of DNA secondary structure observed in vitro should reflect DNA alterations arising in vivo. It has been shown5–9 that the main alteration induced by the fluorene ring is the creation of locally disorganised regions inside the double-helical structure. We now show that S1 endonuclease (EC3.1.4.) from Aspergillus oryzae is able to excise the local regions of denaturation induced in native DNA moleculesby AAAF.
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References
Miller, J. A., Cancer Res., 30, 559–576 (1970).
Kriek, E., Chem. biol. Interactions, 1, 3–7 (1969–70).
Kriek, E., Cancer Res., 32, 2042–2048 (1972).
Irving, C. C., and Veazey, R. A., Cancer Res., 29, 1799–1804 (1969).
Fuchs, R., and Daune, M., Biochemistry, 11, 2659–2666 (1972).
Fuchs, R., and Daune, M., FEBS Lett., 34, 295–298 (1973).
Fuchs, R. P. P., and Daune, M. P., Biochemistry, 13, 4435–4440 (1974).
Fuchs, R., and Daune, M., FEBS Lett., 14, 206–208 (1971).
Levine, A. F., Fink, L. M., Weinstein, I. B., and Grunberger, D., Cancer Res., 34, 319–327 (1974).
Miller, J. A., Sandin, R. B., Miller, E. C., and Rusch, H. P., Cancer Res., 15, 188–199 (1955).
Morris, H. P., Velat, L. A., Wagner, B. P., Dahlgard, N., and Roy, F. E., J. natn. Cancer Inst., 24, 149–180 (1960).
Kriek, E., Miller, J. A., Juhl, U., and Miller, E. C., Biochemistry, 6, 177–182 (1967).
Miller, E. C., Juhl, U., and Miller, J. A., Science, 153, 1125–1127 (1966).
Kapuler, A. M., and Michelson, A. M., Biochim. biophys. Acta, 232, 436–450 (1971).
McConnel, B., and Von Hippel, P. H., J. molec. Biol., 50, 297–316 (1970).
Nelson, J. H., Grunberger, D., Cantor, C. R., and Weinstein, I. B., J. molec. Biol., 62, 331–346 (1971).
Troll, W., Rinde, E., and Day, P., Biochim. biophys. Acta, 174, 211–219 (1969).
Weinstein, I. B., and Grunberger, D., in World Symposium on Model Studies in Chemical Carcinogenesis (edit, by Ts'o, P.O.P. and Di Paolo, J.), 217–235 (Dekker, New York, 1974).
Chang, C. T., Miller, J. J., and Wetmur, J. G., Biochemistry, 13, 2142–2148 (1974).
Weisburger, E. K., and Weisburger, J. H., Adv. Cancer Res., 5, 331–431 (1958).
Cerutti, P. A., Life Sci., 15, 1567–1575 (1975).
Vogt, V. M., Eur. J. Biochem., 33, 192–200 (1973).
Kay, E. R. M., Simmons, N. S., and Dounce, A. L., J. Am. chem. Soc., 74, 1724–1726 (1952).
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FUCHS, R. In vitro recognition of carcinogen-induced local denaturation sites in native DNA by S1 endonuclease from Aspergillus oryzae. Nature 257, 151–152 (1975). https://doi.org/10.1038/257151a0
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DOI: https://doi.org/10.1038/257151a0
