Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

In Vitro Support System for the Study of Blastocyst Differentiation in the Mouse

Abstract

DURING the early implantation stage in the mouse several events in the differentiation of the embryo are initiated. Once the zona pellucida has been lost, attachment to the uterine epithelium occurs, the inner cell mass comes to assume its mesometrial orientation1, trophoblastic giant cells develop, differentiation of the endoderm begins and the inner cell mass undergoes changes leading to egg cylinder formation. The in vitro culture of the attachment stage would allow direct observation and analysis of some of these events and an experimental investigation of the conditions influencing them. Retransfer of eggs to a receptive host animal in order to follow through the effects of experimental manipulations on earlier stages could also, in some cases, be eliminated if in vitro development of early implantation stages could be obtained.

This is a preview of subscription content, access via your institution

Access options

Rent or buy this article

Prices vary by article type

from$1.95

to$39.95

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Kirby, D. R. S., Potts, D. M., and Wilson, I. B., J. Embryol. Exp. Morphol., 17, 527 (1967).

    CAS  PubMed  Google Scholar 

  2. Cole, R. J., and Paul, J., in Pre-Implantation Stages of Pregnancy (edit. by Wolstenholme, G. E. W., and O'Connor, M.), Ciba Foundation Symp. (Churchill, London, 1965).

    Google Scholar 

  3. Kirby, D. R. S., J. Reprod. Fertil., 5, 1 (1963).

    Article  CAS  Google Scholar 

  4. Stevens, L. C. J., Embryol. Exp. Morphol., 20, 339 (1968).

    Google Scholar 

  5. Tarkowski, A. K., J. Embryol. Exp. Morphol., 10, 476 (1962).

    CAS  PubMed  Google Scholar 

  6. Cole, R. J., J. Embryol. Exp. Morphol., 17, 481 (1967).

    CAS  PubMed  Google Scholar 

  7. Potts, D. M., and Wilson, I. B., J. Anat., 102, 1 (1967).

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Carter, S. B., Nature, 206, 1183 (1965).

    Article  ADS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

JENKINSON, E., WILSON, I. In Vitro Support System for the Study of Blastocyst Differentiation in the Mouse. Nature 228, 776–778 (1970). https://doi.org/10.1038/228776a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/228776a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing