Abstract
A COMPOUND of low molecular weight which causes attachment and flattening of cells in culture was prepared from the growth-promoting α-globulin1. The protein from which this low-molecular compound was isolated was prepared by chromatography on ‘DEAE’-cellulose2; in further experiments it was found that this protein could be inactivated by filtration through ‘Sephadex G-25’. Reactivation occurred after incubation of this protein with NaH2PO4 or with NaHCO3; if not incubated with salts, the protein caused flattening of cells in 4–8 h, so that the process of flattening was prolonged.
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Michl, J., Folia biologica (in the press).
Michl, J., Nature, 202, 1133 (1964).
Spector, Jones, M. E., and Lippman, F., in Methods in Enzymology, edit. by Colowick, S. P., and Kaplan, N. O., 3, 653 (Academic Press, New York, 1957).
Michl, J., Exp. Cell Res., 26, 129 (1962).
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MICHL, J. Carbamyl Phosphate as an Essential Component of the Flattening Factor for Cells in Culture. Nature 207, 412 (1965). https://doi.org/10.1038/207412a0
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DOI: https://doi.org/10.1038/207412a0
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