Abstract
OVER the past 50 years many workers have tried to maintain and multiply parenchymal liver cells in tissue culture without success. The following discussion indicates that inadequate oxygen supply may be the basis for this failure. The oxygen tension (pO2) of pooled venous blood is about 40 mm Hg while that of arterial blood is about 95 mm Hg. Therefore the pO2 to which liver cells are exposed in vivo should be somewhere between these values. Direct measurements of liver pO2 in the dog gave a median value of 70 mm Hg1. Let us take 40 mm Hg as the lowest tension in which liver cells can survive.
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Derrick, J. R., and Russell, D., Arch. Surg., 88, 1059 (1964).
Krough, A., J. Physiol., 52, 26 (1919).
Brauer, R. W., Physiol. Rev., 43, 115 (1963).
Allard, C., Mathieu, R., de Lamerande, G., and Cantero, A., Cancer Res., 12, 407 (1952).
Hill, A. V., Proc. Roy. Soc., B., 104, 39 (1928).
Trowell, O. A., Exp. Cell Res., 16, 118 (1959).
Aisenberg, A. C., The Glycolysis and Respiration of Tumors (Academic Press, New York, 1961).
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STEVENS, K. Oxygen Requirements for Liver Cells in vitro. Nature 206, 199 (1965). https://doi.org/10.1038/206199a0
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DOI: https://doi.org/10.1038/206199a0
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