Abstract
THE simultaneous estimation by de Duve1 of hæmo-globin (Hb) and myoglobin (Mb) in solution, based on the spectral characteristics of their carbon monoxide compounds, requires the solutions to be crystal clear and strongly buffered and the relevant wave-lengths must be reproduced to within a fraction of a mµ.
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References
De Duve, C., Acta Chem. Scand., 2, 264 (1948).
Brown, W., Duane Martinez, M., Johnstone, M., and Olcott, H. S., J. Biol. Chem., 237, 81 (1962).
Jonxis, J. H. P., and Visser, N. K. A., Amer. Med. Assoc. J. Diseases Children, 92, 588 (1956).
Colpa-Boonstra, J. P., and Minnaert, K., Biochim. Biophys. Acta, 33, 527 (1959).
Lemberg, R., and Legge, J. W., in Hematin Compounds and Bile Pigments. 214 (Interscience Pub. Inc., New York, 1949).
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WALTERS, C., TAYLOR, A. Differentiation of Myoglobins and Hæmoglobins. Nature 198, 996–997 (1963). https://doi.org/10.1038/198996b0
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DOI: https://doi.org/10.1038/198996b0
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