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Effects of long-term cryopreservation on hematopoietic progenitor cells in umbilical cord blood

Bone Marrow Transplantation volume 23, pages 395–396 (1999)Cite this article

Abstract

There is considerable interest in developing banks of frozen umbilical cord blood cells for transplants but it is uncertain how long frozen cells survive. Our objective was to determine the recovery of frozen umbilical cord blood cells. We quantitated recovery of hematopoietic progenitor cells (CFU-GM, BFU-E, and CFU-GEMM) from frozen umbilical cord blood cells stored for up to 12 years. Decay rates of CFU-GM, BFU-E and CFU-GEMM (d, expressed as percent of viable cells recovered (95% confidence interval) were 0.9930 (0.9889–0.9970), 0.9840 (0.9769–0.9911) and 0.9817 (0.9707–0.9927). Time-dependent recoveries, calculated by the formula dk, (k = frozen storage interval in years) were >90% at 10 years. We conclude that frozen cord blood cells can be stored safely for prolonged intervals without substantial loss in hematopoietic progenitor cells.

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Authors and Affiliations

  1. Department of Pediatrics, Nihon University School of Medicine, Tokyo, Japan

    H Mugishima, K Harada, M Chin & T Suzuki

  2. Department of Gynecology, Nihon University School of Medicine, Tokyo, Japan

    K Takagi, S Hayakawa & K Sato

  3. Division of Biostatistics, Medical College of Wisconsin, Milwaukee, WI, USA

    JP Klein

  4. Salick Health Care, Los Angeles, CA, USA

    RP Gale

Authors
  1. H Mugishima
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  2. K Harada
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  3. M Chin
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  4. T Suzuki
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  5. K Takagi
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  6. S Hayakawa
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  7. K Sato
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  8. JP Klein
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  9. RP Gale
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Mugishima, H., Harada, K., Chin, M. et al. Effects of long-term cryopreservation on hematopoietic progenitor cells in umbilical cord blood. Bone Marrow Transplant 23, 395–396 (1999). https://doi.org/10.1038/sj.bmt.1701580

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  • DOI: https://doi.org/10.1038/sj.bmt.1701580

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