Abstract
We present a method to control protein levels under native genetic regulation in Caenorhabditis elegans by using synthetic genes with adapted codons. We found that the force acting on the spindle in C. elegans embryos was related to the amount of the G-protein regulator GPR-1/2. Codon-adapted versions of any C. elegans gene can be designed using our web tool, C. elegans codon adapter.
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Acknowledgements
We thank E. Busch, M. Decker, N. Goehring and Z. Maliga for helpful discussions.
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Contributions
S.R. characterized all strains. S.S. wrote the web tool algorithm and analyzed genome-wide CAI. S.E. bombarded all constructs. A.P. cloned constructs. S.A. filmed embryos. A.A.H. mentored and financed the project. S.R., A.A.H. and H.B. wrote the paper. H.B. conceived the general synthetic gene design, cloned gpr-1 constructs and preliminarily characterized gpr-1 strains.
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Supplementary Figures 1–8 and Supplementary Table 1 (PDF 4445 kb)
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Redemann, S., Schloissnig, S., Ernst, S. et al. Codon adaptation–based control of protein expression in C. elegans. Nat Methods 8, 250–252 (2011). https://doi.org/10.1038/nmeth.1565
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DOI: https://doi.org/10.1038/nmeth.1565
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