Abstract
Human mitochondrial transcription factor B1 (h-mtTFB1) has an unprecedented relationship to RNA methyltransferases. Here, we show that this protein methylates a conserved stem-loop in bacterial 16S rRNA and that the homologous sequence in the human mitochondrial 12S molecule is similarly modified. Thus, h-mtTFB1 appears to be dual-function protein, acting both as a transcription factor and an rRNA-modification enzyme.
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Acknowledgements
The authors thank A. Vila-Sanjurjo for supplying the ksgA− bacterial strain. This work was supported by an R01 grant awarded to G.S.S. and an National Research Service Award to V.M. from the US National Institutes of Health.
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Seidel-Rogol, B., McCulloch, V. & Shadel, G. Human mitochondrial transcription factor B1 methylates ribosomal RNA at a conserved stem-loop. Nat Genet 33, 23–24 (2003). https://doi.org/10.1038/ng1064
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DOI: https://doi.org/10.1038/ng1064
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