The DNA methyltransferase DNMT1 is essential for cell viability in various mouse models, but gene targeting in human tumor cells results in a viable line. This dilemma has now been resolved using a new human DNMT1 knockout line, although new questions arise as to the full extent of DNMT1 function in maintaining genome integrity.
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Brown, K., Robertson, K. DNMT1 knockout delivers a strong blow to genome stability and cell viability. Nat Genet 39, 289–290 (2007). https://doi.org/10.1038/ng0307-289
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DOI: https://doi.org/10.1038/ng0307-289
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