Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Super-resolution microscopy writ large

Improved expansion microscopy provides a super-resolution method that is accessible to any laboratory with a fluorescence microscope.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Concept and workflow of refined expansion microscopy techniques.

References

  1. Chen, F., Tillberg, P.W. & Boyden, E.S. Science 347, 543–548 (2015).

    Article  CAS  Google Scholar 

  2. Tillberg, P.W. et al. Nat. Biotechnol. 34, 987–992 (2016).

    Article  CAS  Google Scholar 

  3. Ku, T. et al. Nat. Biotechnol. 34, 973–981 (2016).

    Article  CAS  Google Scholar 

  4. Chozinski, T.J. et al. Nat. Methods 13, 485–488 (2016).

    Article  CAS  Google Scholar 

  5. Galbraith, C.G. & Galbraith, J.A. J. Cell Sci. 124, 1607–1611 (2011).

    Article  CAS  Google Scholar 

  6. Richardson, D.S. & Lichtman, J.W. Cell 162, 246–257 (2015).

    Article  CAS  Google Scholar 

  7. Micheva, K.D. & Smith, S.J. Neuron 55, 25–36 (2007).

    Article  CAS  Google Scholar 

  8. Murray, E. et al. Cell 163, 1500–1514 (2015).

    Article  CAS  Google Scholar 

  9. Chen, F. et al. Nat. Methods 13, 679–684 (2016).

    Article  CAS  Google Scholar 

  10. Dodt, H.U. et al. Nat. Methods 4, 331–336 (2007).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors thank the Neurobiology Course at the Marine Biological Laboratory for being their summer home for many years. T.M.'s laboratory is supported by the Deutsche Forschungsgemeinschaft (DFG) through the Munich Center for Systems Neurology (SyNergy; EXC 1010), the Center for Integrated Protein Science Munich (CIPSM, EXC 114), Collaborative Research Center 870, Priority Program 1710 and DFG Research Grant (MI 674/7-1). Further support came from the European Research Council under the European Union's Seventh Framework Program (FP/2007-2013; ERC Grant Agreement n. 616791) and the German-Israeli Foundation. T.M. is also associated with the German Center for Neurodegenerative Diseases (DZNE Munich). P.E. was supported by the DFG Research Training Group 1373 and the Graduate School of the Technische Universität München (TUM-GS); C.F. is supported by the Graduate School of Systemic Neuroscience (GSN, GSC 82).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Thomas Misgeld.

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Engerer, P., Fecher, C. & Misgeld, T. Super-resolution microscopy writ large. Nat Biotechnol 34, 928–930 (2016). https://doi.org/10.1038/nbt.3669

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt.3669

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing