Abstract
SULPHUR is unique among the main elements of living cells in that it is covalently bound to biopolymers but does not occur in the biopolymer backbone. Indeed, most of the bacterial sulphur content resides in the methionine and cysteine side-chains of proteins1. The growth yield of an organism under conditions of sulphur limitation could therefore be greatly enhanced by mutations that substitute Met and Cys in the organism's proteins for sulphur-free amino acids. Because the saving in sulphur would increase with such accumulating mutations, Met and Cys changes could be progressively selected. Abundant proteins should be the prime targets of such a selection. A few published observations give credence to this scenario. Sulphate permease, which is abundantly produced by sulphur-starved Salmonella typhimurium, lacks Met and Cys residues2. Also, two species of marine purple bacteria synthesize more protein than can be expected from a limited sulphate supply3. We now report that the cyanobacterium Calothrix sp. PCC 7601 (referred to here as Calothrix) encodes sulphur-depleted versions of its most abundant proteins—phycocyanin and its auxiliary polypeptides—which it specifically expresses under conditions of sulphur limitation. Although these proteins do not take part in the fixation of sulphur, their elevated synthesis affects the sulphur budget of cyanobacterial cells. Direct evidence is thus provided that the structure of macro molecules can be subject to metabolic optimization.
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References
Roberts, R. B., Cowie, D. B., Abelson, P. H., Bolton, E. T. & Britten, R. V. Studies of Biosynthesis in Escherichia coli Publ. 607 (Carnegie Institute of Washington, Washington, D.C. 1955).
Pardee, A. B. J. biol. Chem. 241, 5886–5892 (1966).
Cuhel, R. L., Taylor, C. D. & Jannasch, H. W. Arch. Mikrobiol. 130, 1–7 (1981).
Stanier, R. Y., Ingraham, J. L., Wheelis, M. L. & Painter, P. R. The Microbial World, 5th edn (Prentice-Hall, Englewood Cliffs 1986).
Tandeau de Marsac, N. Bull. Inst. Pasteur 81, 201–254 (1983).
Glazer, A. N. J. biol. Chem. 264, 1–4 (1989).
Schirmer, T., Bode, W., Huber, R., Sidler, W. & Zuber, H. J. molec. Biol. 184, 257–277 (1985).
Tandeau de Marsac, N. et al. Photosyn. Res. 18, 99–132 (1988).
Mazel, D., Houmard, J. & Tandeau de Marsac, N. Molec. Gen. Genet. 211, 296–304 (1988).
Rippka, R., Deruelles, J., Waterbury, J. B., Herdman, M. & Stanier, R. Y. J. gen. Microbiol. 111, 1–61 (1979).
Klotz, A. V. & Glazer, A. N. J. biol. Chem. 262, 17350–17355 (1987).
Tsunasawa, S., Stewart, J. W. & Sherman, F. J. biol. Chem. 260, 5382–5391 (1985).
Sidler, W., Kumpf, B., Rüdiger, W. & Zuber, H. Biol. Chem. Hoppe-Seyler 367, 627–642 (1986).
Bachmair, A., Finley, D. & Varshavsky, A. Science 234, 179–186 (1986).
Woese, C. R. Microbiol. Rev. 51, 221–271 (1987).
Giovannoni, S. J. et al. J. Bacteriol. 170, 3584–3592 (1988).
Allen, M. B. Arch. Mikrobiol. 32, 270–277 (1959).
Binder, V. A., Locher, P. & Zuber, H. Arch. Hydrobiol. 70, 541–555 (1972).
Creighton, T. E. Proteins: Structures and Molecular Properties. (Freeman, New York 1983).
Bachofen, R. Experientia 42, 1179–1181 (1981).
Frank, G., Sidler, W., Widmer, H. & Zuber, H. Hoppe-Seyler's Z. physiol. Chem. 359, 1491–1507 (1978).
Glazer, A. N. Biochim. biophys. Acta 768, 29–51 (1984).
Belknap, W. R. & Haselkorn, R. EMBO J. 6, 871–884 (1987).
Offner, G. D. & Troxler, R. F. J. biol. Chem. 258, 9931–9940 (1983).
de Lorimier, R. et al. Proc. natl. Acad. Sci. USA 81, 7946–7950 (1984).
Mazel, D. et al. Nucleic Acids Res. 14, 8279–8290 (1986).
Houmard, J., Capuano, V., Coursin, T. & Tandeau de Marsac, N. J. Bacteriol. 170, 5512–5521 (1988).
Matsudaira, P. J. biol. Chem. 262, 10035–10038 (1987).
Guglielmi, G. & Cohen-Bazire, G. Protistologica 20, 393–413 (1984).
Tandeau de Marsac, N. & Houmard, J. Meth. Enzym. 167, 318–328 (1988).
Gross, E. Meth. Enzym. 11, 251–253 (1967).
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Mazel, D., Marlière, P. Adaptive eradication of methionine and cysteine from cyanobacterial light-harvesting proteins. Nature 341, 245–248 (1989). https://doi.org/10.1038/341245a0
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DOI: https://doi.org/10.1038/341245a0
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