Abstract
I HAVE succeeded1 in keeping alive living materials from the cell interiors of which all the easily freezable water was drawn by sufficient extra-cellular freezing at −30° C., after immersion in liquid nitrogen.
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References
Sakai, A., Low Temp. Sci., Ser. B, 14, 17 (1956).
Sakai, A., Low Temp. Sci., Ser. B, 16, 41 (1958).
Asahina, E., and Aoki, K., Nature, 182, 327 (1958).
Asahina, E., Low Temp. Sci., Ser. B, 16, 65 (1958).
Asahina, E. (unpublished).
Nei, T. (unpublished).
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SAKAI, A. Survival of the Twig of Woody Plants at −196° C.. Nature 185, 393–394 (1960). https://doi.org/10.1038/185393a0
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DOI: https://doi.org/10.1038/185393a0
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