Abstract
In the field isolate, 91-58, of feline herpesvirus type 1 (FHV-1), one of the major immunogenic proteins was found to have different molecular masses of 75 and 130 kDa from those in the other field isolates (Maeda et al., J Vet Med Sci 57, 147–150, 1995). Immunoblot analysis using monoclonal antibodies (MAbs) indicated that the protein is glycoprotein C (gC). The gC gene of 91-58 was amplified by polymerase chain reaction (PCR) and shown to have an inserted fragment of approximately 160 base pairs (bp). Restriction endonuclease analysis of the PCR product with various restriction enzymes was carried out, indicating that the insertion located within 262 bp between EcoRV and DraI sites. Nucleotide sequence analysis indicated that the inserted fragment was 156 bp encoding 52 amino acids and composed repeat sequences. Next, five recent isolates were also examined by immunoblot analysis using anti-FHV-1 cat serum or MAbs. The result showed that one isolate, 98-064, also had the gC with different molecular weights. PCR and nucleotide sequence analyses indicated that 98-064 had an inserted sequence of 78 bp at the corresponding region identified in the gC gene of 91-58, although the inserted sequence was different from that of 91-58. These results indicated that some of FHV-1 isolates had the genetic rearrangements in the gC gene and detection of such mutations would be useful for differentiation among FHV-1 field isolates.
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Ditchfield J., and Grinyer I., Virology 26, 504–506, 1965.
Povey R.C., Comp Immunol Microbiol Infect Dis 2, 373–387, 1970.
Studdert M.J., and Martin M.C., Aust Vet J 46, 99–105, 1970.
Bittle J.L., and Rubic W., Am J Vet Res 36, 89–91, 1975.
Fargeaud D., Jeannin C.B., Kato F., and Chappuis G., Arch Virol 80, 69–82, 1984.
Gaskell R., and Willoughby K., Vet Microbiol 69, 73–88, 1999.
Stiles J., Vet Clin North Am Small Anim Pract 30, 1001–1014, 2000.
Spatz S.J., Rota P.A., and Maes R.K., J Gen Virol 75, 1235–1244, 1994.
Willemse M.J., Strijdveen I.G.L., van Schooneveld S.H.B., van den Berg M.C., and Sondermeijer P.J.A., Virology 208, 704–711, 1995.
Maeda K., Horimoto T., and Mikami T., J Vet Med Sci 60, 881–888, 1998.
Maeda K., Kawaguchi Y., Ono M., Inoshima Y., Miyazawa T., Tohya Y., Kai C., and Mikami T., Virology 202, 1034–1038, 1994.
Maeda K., Hirasawa K., Kawaguchi Y., Ono M., Mori T., Gemma T., Yokoyama N., Doi K., and Mikami T., Virus Res 39, 55–61, 1995.
Maeda K., Ono M., Kawaguchi Y., Niikura M., Okazaki K., Yokoyama N., Tokiyoshi Y., Tohya Y., and Mikami T., Virus Res 46, 75–80, 1996.
Maeda K., Ono M., Kawaguchi Y., Okazaki K., Yokoyma N., Tohya Y., and Mikami T., J Vet Med Sci 59, 217–219, 1997.
Maeda K., Yokoyama N., Fujita K., and Mikami T., Virus Genes 14, 105–109, 1997.
Maeda K., Xuan X., Kawaguchi Y., Ono M., Yokoyama N., Fujita K., Tohya Y., and Mikami T., J Vet Med Sci 59, 1003–1009, 1997.
Maeda K., Yokoyama N., Fujita K., Maejima M., and Mikami T., Virus Res 52, 169–176, 1997.
Maeda K., Kawaguchi Y., Ono M., Tajima T., and Mikami T., J Clin Microbiol 33, 217–221, 1995.
Maeda K., Kawaguchi Y., Ono M., Tajima T., and Mikami T., J Vet Med Sci 57, 147–150, 1995.
Horimoto T., Limcumpao J.A., Xuan X., Ono M., Maeda K., Kawaguchi Y., Kai C., Takahashi E., and Mikami T., Arch Virol 126, 283–292, 1992.
Hamano M., Maeda K., Mizukoshi F., Une Y., Mochizuki M., Tohya Y., Akashi H., and Kai K., J Vet Med Sci 65, 939–943, 2003.
Mochizuki M., Konishi S., and Ogata M., Jpn J Vet Sci 39, 27–37, 1977.
Mochizuki M., Konishi S., and Ogata M., Jpn J Vet Sci 39, 191–194, 1977.
Crandell R.A., Fabricant C.G., and Nelson-Ress W.A., In Vitro 9, 176–185, 1973.
Horimoto T., Limcumpao J.A., Tohya Y., Takahashi E., and Mikami T., Arch Virol 111, 127–132, 1990.
Umene K., Herpesvirus Genetic Variability and Recombination. Touka Shobou, Fukuoka, 1998, pp. 131–139, 196.
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Hamano, M., Maeda, K., Mizukoshi, F. et al. Genetic Rearrangements in the gC Gene of the Feline Herpesvirus Type 1. Virus Genes 28, 55–60 (2004). https://doi.org/10.1023/B:VIRU.0000012263.87632.1d
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DOI: https://doi.org/10.1023/B:VIRU.0000012263.87632.1d