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Evaluation of a PCR Based on Primers to Nc5 Gene for the Detection of Neospora caninum in Brain Tissues of Bovine Aborted Fetuses

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REFERENCES

  • Baszler, T.V., Gay, L.J.C., Long, M.T. and Mathison, B., 1999. Detection by PCR of Neospora caninum in fetal tissues from spontaneous bovine abortions. Journal of Clinical Microbiology, 37, 4059–4064

    PubMed  CAS  Google Scholar 

  • Björkman, C., Johansson, O., Stenlund, S., Holmdahl, O.J.M. and Uggla, A., 1996. Neospora species infection in a herd of dairy catle. Journal of the American Veterinary Medical Association, 208, 1441–1444

    PubMed  Google Scholar 

  • Chomczynski, P., 1993. A reagent for the single step simultaneous isolation of RNA, DNA and proteins from cells and tissue samples. Biotechniques, 15, 532–537

    PubMed  CAS  Google Scholar 

  • De Marez, T., Liddell, S., Dubey, J.P., Jenkins, M.C. and Gasbarre, L., 1999. Oral infection of calves with Neospora caninum oocysts from dogs: humoral and cellular immune responses. International Journal of Parasitology, 29, 1647–1657

    Article  PubMed  CAS  Google Scholar 

  • Ellis, J.T., 1998. Polymerase chain reaction approaches for the detection of Neospora caninum and Toxoplasma gondii. International Journal for Parasitology, 28, 1053–1060

    Article  PubMed  CAS  Google Scholar 

  • Ellis, J.T., McMillan, D., Ryce, C., Payne, S., Atkinson, R. and Harper, P.A., 1999. Development of a single tube nested polymerase chain reaction assay for the detection of Neospora caninum DNA. International Journal for Parasitology, 29, 1589–1596

    Article  PubMed  CAS  Google Scholar 

  • Ho, M.S.Y., Barr, B.C., Rowe, J.D., Anderson, M.L., Sverlow, K.W., Packham, A.E., Marsh, A.E. and Conrad, P.A., 1997. Detection of Neospora sp. from infected bovine tissues by PCR and probe hybridization. Journal of Parasitology, 83, 508–514

    Article  PubMed  CAS  Google Scholar 

  • Jenkins, M., Baszler, T., Björkman, C., Schares, G. and Williams, D., 2002. Diagnosis and seroepidemiology of Neospora caninum-associated bovine abortion. International Journal for Parasitology, 32, 631–636

    Article  PubMed  Google Scholar 

  • Lally, N.C., Jenkins, M.C. and Dubey, J.P., 1996. Development of a polymerase chain reaction assay for the diagnosis of neosporosis using the Neospora caninum 14-3-3 gene. Molecular and Biochemical Parasitology, 75, 169–178

    Article  PubMed  CAS  Google Scholar 

  • Moen, A.R., Wouda, W., Mul, M.F., Graat, E.A.M. and Vanwerven, T., 1998. Increased risk of abortion following Neospora caninum abortion outbreaks: a retrospective and prospective cohort study in four dairy herds. Theriogenology, 49, 1301–1309

    Article  PubMed  CAS  Google Scholar 

  • Müller, N., Zimmermann, V., Hendrich, B. and Gottstein, B., 1996. Diagnosis of Neospora caninum and Toxoplasma gondii infection by PCR and DNA hybridization immunoassay. Journal of Clinical Microbiology, 34, 2850–2852

    PubMed  Google Scholar 

  • Paré, J., Thurmond, M.C. and Hietala, S.K., 1996. Congenital Neospora caninum infection in dairy cattle and associated calfhood mortality. Canadian Journal of Veterinary Research, 60, 133–139

    PubMed  Google Scholar 

  • Sambrook, J., Fritsch, E.F. and Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual, 2nd edn, (Cold Spring Harbor, NY: Cold Spring Harbor Press)

    Google Scholar 

  • Thurmond, M.C. and Hietala, S.K., 1997. Effect of congenitally acquired Neospora caninum infection on risk of abortion and subsequent abortions in dairy cattle. American Journal of Veterinary Research, 58, 1381–1385

    PubMed  CAS  Google Scholar 

  • Thurmond, M.C., Hietala, S.K. and Blanchard, P.C., 1999. Predictive values of foetal histopathology and immunoperoxidase staining in diagnosing bovine abortion caused by Neospora caninum in a dairy herd. Journal of Veterinary Diagnostic Investigation, 11, 90–94

    PubMed  CAS  Google Scholar 

  • Yamage, M., Fletchner, O. and Gottstein, B., 1996. Neospora caninum: specific oligonucleotide primers for the detection of brain ‘cyst’ DNA of experimentally infected nude mice by the polymerase chain reaction (PCR). Journal of Parasitology, 82, 272–279

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia da Universidade de Sao Paulo, Rua Prof. Dr. Orlando Marques de Paiva 87, CEP 05508-000 Cidade Universitária, Sa~o, Paulo-SP, Brasil

    V.S.O. Paula, A.A.R. Rodrigues, L.J. Richtzenhain, A. Cortez, R.M. Soares & S.M. Gennari

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  1. V.S.O. Paula
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  2. A.A.R. Rodrigues
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  3. L.J. Richtzenhain
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  4. A. Cortez
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  5. R.M. Soares
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  6. S.M. Gennari
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Paula, V., Rodrigues, A., Richtzenhain, L. et al. Evaluation of a PCR Based on Primers to Nc5 Gene for the Detection of Neospora caninum in Brain Tissues of Bovine Aborted Fetuses. Vet Res Commun 28, 581–585 (2004). https://doi.org/10.1023/B:VERC.0000042877.07684.89

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  • DOI: https://doi.org/10.1023/B:VERC.0000042877.07684.89

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