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The Effect of Selected Monoterpenoids on the Cellular Slime Mold, Dictyostelium discoideum NC4

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Abstract

We tested the activity of 11 main compounds identified from Pinus plants on the growth of Dictyostelium discoideum NC4. Four concentrations (1, 0.1, 0.01, 0.001 μg/μl) of each compound were tested using a disk volatilization technique following germination of D. discoideum NC4 spores. Photographs of D. discoideum NC4 fruiting bodies were taken 2 days after treatment. Fenchone (at 0.1, 0.01, and 0.001 μg/μl) and camphene (at 0.01 μg/μl) stimulated growth of D. discoideum NC4. (1S)-(−)-verbenone, (1S)-(−)-α-pinene, (+)-β-pinene, myrcene, (−)-menthone, (−)-bornyl acetate, (S)-(+)-carvone, (−)-camphene, and (R)-(+)-limonene inhibit its growth. All of the compounds at 1 μg/μl had a strong inhibitory effect on cell growth of D. discoideum NC4. Microscopic observation of the fruiting bodies matched the results of growth rate analysis. Most of the inhibitory effects were represented by changes in the shapes of the fruiting bodies. These changes include short sorophores, smaller sized sori, and sori without spores. Our results suggest that inhibition of growth is the most common effect of monoterpenoids on D. discoideum NC4. Nevertheless, some of them, like fenchone and camphene, seem to enhance its growth.

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Authors and Affiliations

  1. Department of Biology, Kyungnam University, Masan, 631-701, Korea

    J. Y. Hwang & J. H. Kim

  2. Department of Oriental Medicine Resources, Sunchon National University, Sunchon, 540-742, Korea

    K. W. Yun

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  1. J. Y. Hwang
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  2. J. H. Kim
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  3. K. W. Yun
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Hwang, J.Y., Kim, J.H. & Yun, K.W. The Effect of Selected Monoterpenoids on the Cellular Slime Mold, Dictyostelium discoideum NC4. J Chem Ecol 30, 1153–1163 (2004). https://doi.org/10.1023/B:JOEC.0000030269.07833.8f

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  • DOI: https://doi.org/10.1023/B:JOEC.0000030269.07833.8f

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