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Glycosphingolipids govern gene expression

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Abstract

To elucidate the biological significance of the lactosylceramide (LacCer) branching in glycosphingolipid (GSL) biosynthesis, we established ganglioside GM3- and lactosylsulfatide SM3-reconstituted cells by introducing the GM3 synthase gene and the sulfotransferase gene, respectively. In SM3-expressing cells, the reduction of β1 integrin mRNA expression, the reduced adhesivity to fibronectin and laminin, and the suppression of anchorage-independent growth (tumorigenic potential) were observed. On the other hand, in GM3-expressing cells, anchorage-independent growth was promoted and the expression of PDGFα receptor mRNA was specifically reduced. Interestingly enough, no change in anchorage-dependent growth was observed in these cells, and tumorigenic signals were controlled selectively in both positive and negative directions. Thus, the spatio-temporal, gene expression control mechanism by individual GSL molecules accumulating in the cell membrane microdomain (raft) has been proven. Published in 2004.

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Authors and Affiliations

  1. Department of Biomembrane and Biofunctional Chemistry, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12-jo, Nishi 6-chome, Kita-ku, Sapporo, 060-0812, Japan

    Jin-ichi Inokuchi, Kazuya Kabayama, Satoshi Uemura & Yasuyuki Igarashi

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  1. Jin-ichi Inokuchi
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  2. Kazuya Kabayama
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  3. Satoshi Uemura
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  4. Yasuyuki Igarashi
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Correspondence to Jin-ichi Inokuchi.

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Inokuchi, Ji., Kabayama, K., Uemura, S. et al. Glycosphingolipids govern gene expression. Glycoconj J 20, 169–178 (2003). https://doi.org/10.1023/B:GLYC.0000024248.62242.1f

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  • DOI: https://doi.org/10.1023/B:GLYC.0000024248.62242.1f

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