Abstract
Chromosome specific painting probes of human, sheep and the Hirola antelope (Damaliscus hunteri) derived by flow sorting of chromosomes were used in multi directional chromosome painting experiments to better define the karyological relationship within Bovidae species (specifically, Caprini and Alcelaphini tribes) and humans. Although not all chromosomes of Damaliscus hunteri could be resolved into single peaks by flow-sorting we managed to present a complete homology map for chromosomes between the three species. When comparing the karyotype of Damaliscus hunteri with human all of the main known motives in mammalian chromosome evolution are present (i.e. associations of human homologous chromosomes 3–21, 4–8, 7–16, 14–15, 16–19 and two forms of 12–22) which were also confirmed with the sheep paint probes. Further, we observed those patterns that have been described as common derived traits for artiodactyls (i.e. associations of human homologous chromosomes 5/19 and a complex alternating pattern of hybridizations with human chromosome 14 and 15 probes). As known from classical karyotyping some of the Damaliscus chromosomes are biarmed and were supposedly involved in Robertsonian translocations frequently found in karyotype evolution of bovids. We refined these rearrangements with the molecular probes and also delineated a chromosome painting pattern that should be the result of a paracentric inversion in the Damaliscus hunteri karyotype. This study demonstrates that multidirectional chromosome painting will be a valuable tool for the investigation of the dynamics of chromosome evolution in exotic bovid species.
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References
Ansell WFH (1977) Order Artiodactyla. Part 15. In: Meester J, Setzer HW, eds. The Mammals of Africa. An identification Manual. Washington DC: Smithsonian Institution Press, pp 1–84.
Buckland RA, Evans HJ(1978) Cytogenetic aspects of phylogeny in the Bovidae I. G-banding. Cytogenet Cell Genet 21: 42–63.
Burkin DJ, O'Brien PCM, Broad TE et al. (1997) Isolation of chromosome-specific paints from high-resolution flow karyotypes of the sheep (Ovis aries). Chromosome Res 5: 102–108.
Chaves R, Adega F, Santos S, Heslop-Harrison JS, Guedes-Pinto H (2002) In situ hybridization and chromosome banding in mammalian species. Cytogenet Genome Res 96: 113–116.
Chowdhary BP, Fronicke L, Gustavsson I, Scherthan H (1996) Comparative analysis of the cattle and human genomes, detection of ZOO-FISH and gene mapping-based chromosomal homologies. Mamm Genome 7: 297–302.
Chowdhary BP, Raudsepp T, Fronicke L, Scherthan H (1998) Emerging patterns of comparative genome organization in some mammalian species as revealed by Zoo-FISH. Genome Res 8: 77–589.
Crawford AM, Dodds KG, Ede AJ et al. (1995) An autosomal genetic linkage map of the sheep genome. Genetics 140: 703–724.
de Gortari MJ, Freking BA, Cuthbertson RP et al. (1998) A second-generation linkage map of the sheep genome. Mamm Genome 9: 204–209.
East R (1995) Antelope survey update. Number 1. IUCN/SSC Antelope Specialist Group Report. Gland, Switzerland: International Union for Conservation of Nature and Natural Resources.
Effron M, Bogart MH, Kumamoto AT, Benirschke K (1976) Chromosome studies in the mammalian subfamily Antilopinae. Genetica 46: 419–444.
Frönicke L, Wienberg J (2001) Comparative chromosome painting defines the high rate of karyotype changes between pigs and bovids. Mamm Genome 12: 442–449.
Gallagher DS, Womack JE (1992) Chromosome conservation in the Bovidae. J Hered 82: 287–298.
Gallagher DS, Derr JN, Womack JE (1994) Chromosome conservation among the advanced pecorans and determination of the primitive bovid karyotype. J Hered 85: 204–210.
Hayes H (1995) Chromosome painting with human chromosome-specific DNA libraries reveals the extent and distribution of conserved segments in bovine chromosomes. Cytogenet Cell Genet 71: 168–174.
Iannuzzi L, Di Meo GP, Perucatti A, Bardaro T (1998) ZOOFISH and R-banding reveal extensive conservation of human chromosome regions in euchromatic regions of river buffalo chromosomes. Cytogenet Cell Genet 82: 210–214.
Iannuzzi L, Di Meo GP, Perucatti A, Incarnato D (1999) Comparison of the human with the sheep genomes by use of human chromosome-specific painting probes. Mamm Genome 10: 719–723.
Iannuzzi L, Di Meo GP, Perucatti A, Schibler L, Incarnato D, Cribiu EP (2001) Comparative FISH-mapping in river buffalo and sheep chromosomes: assignment of forty autosomal type I loci from sixteen human chromosomes. Cytogenet Cell Genet 94: 43–48.
ISCNDA (1989) International System for Cytogenetic Nomenclature of Domestic Animals. In: Di Berardino D, Hayes H, Fries R, Long S, eds. Cytogenet Cell Genet 53: 65–79.
ISCNDB (2000) International System for Chromosome Nomenclature of Domestic. In: Bovids, Di Berardino D, Di Meo GP, Gallagher DS, Hayes H, Iannuzzi L, eds. Cytogenet Cell Genet 92: 283–299 (2001).
Kingdon J (1982) East African Mammals; An Atlas of Evolution in Africa, Vol. IIID (Bovids). Chicago: University of Chicago Press.
Kumamoto AT, Charter SJ, Houck ML, Frahm M (1996) Chromosomes of Damaliscus (Artiodactyla, Bovidae): simple and complex centric fusion rearrangements. Chromosome Res 4: 614–621.
Nowak RM (1991) Walker's Mammals of the World, 5th edn., Vol. II. Baltimore: Johns Hopkins University Press.
Pinkel D, Straume T, Gray JH (1986) Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA 86: 2934–2938.
Rabbits P, Impey H, Heppell-Parton A et al. (1995) Chromosome specific paints from a high resolution flow karyotype of the mouse. Nat Genet 9: 369–375.
Schmitz A, Oustry A, Vaiman D, Chaput B, Frelat G, Cribiu EP (1998) Comparative karyotype of pig and cattle using whole chromosome painting probes. Hereditas 128: 257–263.
Solinas-Toldo S, Lengauer C, Fries R (1995) Comparative genome map of human and cattle. Genomics 27: 489–496.
Vaiman D, Schibler L, Bourgeois F, Oustry A, Amigues Y, Cribiu EP (1996) A genetic linkage map of the male goat genome. Genetics 144: 279–305.
Wienberg J, Stanyon R, Jauch A, Cremer T (1992) Homologies in human and Macaca fuscata chromosomes revealed by in situ suppression hybridization with human chromosome specific DNA libraries. Chromosoma 101: 265–270.
Wienberg J, Stanyon R, Nash WG et al. (1997) Conservation of human vs. feline genome organisation revealed by reciprocal chromosome painting. Cytogenet Cell Genet 77: 211–217.
Wienberg J, Frönicke L, Stanyon R (2000) Insights into mammalian genome organization and evolution by molecular cytogenetics. In: Clark MS, ed. Comparative Genomics. Dordrecht, Netherlands: Kluwer Academic Publishers, pp 207–244.
Wurster DH, Benirschke K (1968) Chromosome studies in the superfamily Bovidae. Chromosoma 25: 152–171.
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Chaves, R., Frönicke, L., Guedes-Pinto, H. et al. Multidirectional chromosome painting between the Hirola antelope (Damaliscus hunteri, Alcelaphini, Bovidae), sheep and human. Chromosome Res 12, 495–503 (2004). https://doi.org/10.1023/B:CHRO.0000034751.84769.4c
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DOI: https://doi.org/10.1023/B:CHRO.0000034751.84769.4c