biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:321-325, 2003 | DOI: 10.1023/B:BIOP.0000023872.61646.95

Simultaneous Regeneration of Different Morphogenic Structures from Quince Leaves as Affected by Growth Regulator Combination and Treatment Length

C. D'Onofrio1, S. Morini1,*
1 Dipartimento di Coltivazione e Difesa delle Specie Legnose "G. Scaramuzzi", Sezione Coltivazioni Arboree, Università di Pisa, Pisa, Italy

Experiments were performed to evaluate the capacity of quince (Cydonia oblonga Mill.) leaves to regenerate somatic embryos and shoots and/or roots simultaneously. Leaves, treated for 2 d in liquid medium containing 2.5 mg dm-3 2,4-dichlorophenoxyacetic acid were cultured for 0, 3, 6, 9, 12, 15, 18, 21 d on a gelled medium supplemented with 1 mg dm-3 kinetin (Kin) and 0.1 mg dm-3 naphthalenacetic acid (NAA) and were transferred to a medium either without growth regulator (GR-) or containing 0.6 mg dm-3 6-benzylaminopurine (BA) + 0.2 mg dm-3 gibberellic acid (GA3) + 0.06 mg dm-3 indole-3-butyric acid (IBA) (GR+). Leaves producing somatic embryos (SEs) only, or adventitious roots (Rs) only, or SEs+Rs simultaneously, were detected on GR- culture medium; on GR+ medium, leaves producing adventitious shoots (Ss) only, SEs+Ss or SEs+Rs+Ss simultaneously, also appeared. Leaves producing both Ss+Rs were never detected. Proportions among the various types of regenerating leaves varied according to both the length of Kin+NAA treatment and the presence or absence of GR in the transfer medium. The greatest variations, both on GR- and on GR+, took place within the first 9 d of culturing on Kin+NAA. After this period, no further substantial differences in the trend of each type of regenerating leaf were observed. The length of the treatment with Kin+NAA also modified the proportions between the different types of morphogenic structures.

Keywords: adventitious shoot; adventitious root; cytokinin; Cydonia oblonga; morphogenesis; somatic embryo
Subjects: auxins; Cydonia oblonga; cytokinins; in vitro culture, nutrient media modification; in vitro culture, regeneration, organogenesis; in vitro culture, somatic embryogenegis; quince; root, adventitious

Published: November 1, 2003  Show citation

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D'Onofrio, C., & Morini, S. (2003). Simultaneous Regeneration of Different Morphogenic Structures from Quince Leaves as Affected by Growth Regulator Combination and Treatment Length. Biologia plantarum, 46(3), 321-325. doi: 10.1023/B:BIOP.0000023872.61646.95
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