Abstract
This study evaluated an automated immunoassay for bovine lactoferrin (LF) in dairy products based on latex beads coated with F(ab′)2 fragments. Methods: F(ab′)2 fragments were obtained by pepsin digestion of rabbit anti-bovine LF (IgG fraction) and polystyrene latex beads were coated with the F(ab′)2 fragments. We used the beads to develop a rapid and homogeneous light scatter immunoassay employing an autoanalyzer (the Automated Latex assay). The Automated Latex assay was easy to perform and could rapidly determine bovine lactoferrin in lactoferrin-supplemented products. It was sensitive enough for testing products and showed good precision.
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Yamauchi, K., Soejima, T., Ohara, Y. et al. Rapid determination of bovine lactoferrin in dairy products by an automated quantitative agglutination assay based on latex beads coated with F(ab′)2 fragments. Biometals 17, 349–352 (2004). https://doi.org/10.1023/B:BIOM.0000027716.96028.84
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DOI: https://doi.org/10.1023/B:BIOM.0000027716.96028.84